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PANOSSIAN, ZEHBOUR; COSTA, ISOLDA
; ROSSI, JESUALDO L.
.
ICC INTERCORR WCO 2021: A Virtual Edition.
Materials Research,
v. 25,
suppl. 1,
2022.
DOI:
10.1590/1980-5373-MR-2023-E002
Abstract:
Brazil, once again, organized one of the world’s most significant corrosion events, the ICC - INTERNATIONAL CORROSION CONGRESS. In 1978, the 7th edition of the congress was held by ABRACO, in Rio de Janeiro, under the direction of our Dr. Aldo Cordeiro Dutra, chairman of the Executive Committee. To intertwine two events, the 21st ICC was held together with the traditional INTERCORR, the most representative corrosion and corrosion prevention event held in Brazil, organized by ABRACO, in its 8th edition, which included the workshop “Corrosion and Green Energy”, an event of the World Corrosion Organization (WCO). Thus, the ICC INTERCORR WCO 2021 was arranged with this strong partnership.The institutions responsible for organizing the ICC INTERCORR WCO 2021 were: the Brazilian Corrosion Association (ABRACO), the International Corrosion Council (ICC), and the World Corrosion Organization (WCO). To overcome a new post-pandemic Covid-19 reality, the event occurred virtually from July 20th to 23rd, 2021.The ICC INTERCORR WCO 2021 has become a reference for developing the industrial sector, being an excellent forum for presenting new technologies, disseminating knowledge, prospecting for brands, business opportunities and expanding relationships. Its main objective was to encourage the exchange of knowledge between researchers, technicians, professionals, and companies, whether nationally or globally.The ICC INTERCORR WCO 2021 program included the presentation of 6 plenary conferences, 19 lectures, and more than 220 technical articles by renowned specialists from different countries such as Germany, India, Egypt, USA, Portugal, Australia, Italy, Sweden, Spain, Brazil, among others.Today´s moment highlights the importance of promoting such an event in the country, aiming to find solutions to contribute to the recovery that the industrial sector needs. Maintaining the integrity of assets and minimizing operating and maintenance costs are highly relevant concerns in our daily lives, directly impacting the productive sector.Therefore, on behalf of the Executive Committee, we would like to thank all participants and members of the local organizing committee and the scientific committee. We also sincerely thank the support team for collaborating before, during and after this highly successful event.
PANOSSIAN, ZEHBOUR; COSTA, ISOLDA; ROSSI, JESUALDO L.
ICC INTERCORR WCO 2021: A Virtual Edition.
Materials Research,
v. 25,
2022.
suppl. 1,
DOI:
10.1590/1980-5373-MR-2023-E002.
Disponível em: http://repositorio.ipen.br/handle/123456789/34187. Acesso em: $DATA.
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GOMES, ANDERSON S.L.; SOUZA, EUNEZIO T. de; ZEZELL, DENISE M.
.
Latin America Optics and Photonics 2022: introduction to the feature issue.
Applied Optics,
v. 62,
n. 8,
p. 1-2,
2023.
DOI:
10.1364/AO.489414
Abstract:
The 2022 Latin America Optics and Photonics Conference (LAOP 2022), the major international conference
sponsored byOptica in Latin America, returned to Recife, Pernambuco, Brazil, after its first edition in 2010.Held
every two years since (except for 2020), LAOP has the explicit objective to promote Latin American excellence in
optics and photonics research and support the regional community. In the 6th edition in 2022, it featured a comprehensive
technical program with recognized experts in fields critical to Latin America, highly multidisciplinary,
with themes frombiophotonics to2Dmaterials. The 191 attendees of LAOP2022 listened to five plenary speakers,
28 keynotes, 24 invited talks, and 128 presentations, including oral and posters
Palavras-Chave:
optical activity;
photon emission;
lasers;
fiber optics;
meetings
GOMES, ANDERSON S.L.; SOUZA, EUNEZIO T. de; ZEZELL, DENISE M.
Latin America Optics and Photonics 2022: introduction to the feature issue.
Applied Optics,
v. 62,
n. 8,
p. 1-2,
2023.
DOI:
10.1364/AO.489414.
Disponível em: http://repositorio.ipen.br/handle/123456789/34097. Acesso em: $DATA.
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BRANDAO, ULISSES
; SPINACE, ESTEVAM V.
; SEKO, MURILO H.; FERREIRA, WILLIAM de P..
Projeto e construção de um sistema de aquecimento por micro-ondas de baixo custo para avaliação de susceptores.
Anais do Workshop de Micro-ondas,
v. 1,
n. 6,
p. 1-4,
2022.
DOI:
10.5281/zenodo.7857092
Abstract:
Este trabalho consistiu no projeto e construção de um forno de micro-ondas de 2,45 GHz, destinado à avaliação de susceptores a serem utilizados como catalisadores, em temperaturas de 400 a 1.100 ºC. Sua construção baseou-se em simulações feitas em computador, seguida de testes e modificações para sua funcionalização.
BRANDAO, ULISSES; SPINACE, ESTEVAM V.; SEKO, MURILO H.; FERREIRA, WILLIAM de P.
Projeto e construção de um sistema de aquecimento por micro-ondas de baixo custo para avaliação de susceptores.
Anais do Workshop de Micro-ondas,
v. 1,
n. 6,
p. 1-4,
2022.
DOI:
10.5281/zenodo.7857092.
Disponível em: http://repositorio.ipen.br/handle/123456789/34095. Acesso em: $DATA.
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MORALES, J.M.G.; TORTAMANO-NETO, P.; TODESCAN, F.F.; ANDRADE JUNIOR, J.C.S. de; MAROTTI, J.; ZEZELL, D.M.
.
Resonance frequency analysis of dental implants after laser therapy.
Clinical Oral Implants Research,
v. 21,
n. 10,
p. 1045-1045,
2010.
DOI:
10.1111/j.1600-0501.2010.01994.x
Abstract:
Background: The importance of primary stability in implant
placement for long-term success is well known in the literature.
The resonance frequency analysis technique has extensively
been used in experimental and clinical research for the last 10
years, for assessing primary stability. The benefits of low-level
laser therapy in bone tissue are already consolidated in the
literature, however, little is known about its benefits on improvement
of stability of dental implants.
Aim: The aim of this randomdouble blind clinical study was to
investigate whether stability of titanium implants can be enhanced
by low-level laser therapy during the osseointegration
process when measured by means of resonance frequency
analysis. Methods: Thirty implants (n¼30) were placed in the posterior
region of partially edentulous mandible of eight adult patients,
after following several inclusion criteria and were distributed
bilaterally in agreement with the prosthetic requirement. The
implants on the experimental side were randomly submitted to
low-level laser therapy (830 nm, 86mW, 92.1 J/cm2, 2.57 J, 3 s/
point, at 20 points), and on the control side the laser irradiation
was only simulated. The first irradiation was performed in the
immediate postoperative period, and it was repeated every 48 h
in the first 14 days. The initial implant stability quotient of the
implants was measured by means of resonance frequency analyzer
(Osstells). New implant stability quotient measurements
were made after 10 days, 3, 6, 9 and 12 weeks. ANOVA for
repeated measurements and Bonferroni test were used to assess
statistically significant differences. Results: The initial implant stability quotient values ranged
from 65 to 84, with a mean of 76. The irradiated side showed
significant drop in stability from the 10th day until the sixth
week only (P¼0.028), and presenting a gradual increase from
the sixth to the 12th week. In the control side, the mean implant
stability quotient increased up to the third week, decreased in
the sixth week, and then began to grow again. The highest
stability values were observed in the 10th day in the irradiated
group, and the lowest in the sixth week in both groups. No
statistical differences were detected among the ISQ means in
the two groups for each of the six times observation.
Conclusions and clinical implications: No evidence was found of
any effect of low-level laser therapy on implants’ stability when
measured by resonance frequency analysis. Because high primary
stability and good bone quality are of major relevancy for a
rigid bone–implant interface, additional low-level laser therapy under these conditions may have little impact macroscopically.
Further researches under different bone conditions are necessary
for a better understanding of the occurrences at bone/implant
interface.
MORALES, J.M.G.; TORTAMANO-NETO, P.; TODESCAN, F.F.; ANDRADE JUNIOR, J.C.S. de; MAROTTI, J.; ZEZELL, D.M.
Resonance frequency analysis of dental implants after laser therapy.
Clinical Oral Implants Research,
v. 21,
n. 10,
p. 1045-1045,
2010.
DOI:
10.1111/j.1600-0501.2010.01994.x.
Disponível em: http://repositorio.ipen.br/handle/123456789/34053. Acesso em: $DATA.
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ANA, P.A.
; TABCHOURY, C.P.M.; CURY, J.A.; ZEZELL, D.M.
.
Effect of Er,Cr:YSGG laser and fluoride application on enamel demineralization.
Caries Research,
v. 41,
n. 4,
p. 325-326,
2007.
DOI:
10.1159/000103637
Abstract:
This study evaluated the influence of sub-ablative Er,Cr:YSGG
laser and topical fluoride application on incipient caries development
in vitro. One hundred and sixty human enamel slabs were
ramdomly divided into eight equal groups: (1) untreated (control);
(2–4) irradiated with Er,Cr:YSGG laser at 0.25, 0.50 and 0.75
W, respectively; (5) treated with acidulated phosphate fluoride
(APF; 1.23% F) for 4 min; (6–8) pre-irradiated with Er,Cr:YSGG
laser at 0.25, 0.50 and 0.75 W, respectively and subjected to APF
application. All groups were submitted to pH-cycling simulating
a cariogenic challenge, and after 10 cycles mineral loss ( Δ Z) was
measured in enamel. Calcium, inorganic phosphorus (P i ) and fluoride
concentrations were also measured in the demineralizing and remineralizing pH-cycling solutions. Data were analyzed by
ANOVA ( α = 0.05). No differences in Δ Z were found between laser
irradiation at 0.25 W, 0.50 W and controls, while after laser
irradiation at 0.75 W (Group 4) and application of APF (Group 5)
there was a significant decrease in Δ Z. Laser irradiation at 0.50
and 0.75 W prior to APF application there was a significantly lower
Δ Z than in other groups. A significant increase in Ca and P i
concentrations was observed in demineralizing solutions compared
to the initial concentrations, but no significant changes in
Ca and P i concentrations were detected between any groups. A
significant decrease in fluoride concentration was observed in
pH-cycling solutions of control and 0.25 W laser groups, while the
association of laser at 0.50 and 0.75 W with fluoride promoted an
increase of fluoride content in remineralizing solutions. In conclusion,
Er,Cr:YSGG laser irradiation at 0.50 and 0.75 W, associated
with fluoride, seems to be a good alternative for reducing
enamel demineralization.
ANA, P.A.; TABCHOURY, C.P.M.; CURY, J.A.; ZEZELL, D.M.
Effect of Er,Cr:YSGG laser and fluoride application on enamel demineralization.
Caries Research,
v. 41,
n. 4,
p. 325-326,
2007.
DOI:
10.1159/000103637.
Disponível em: http://repositorio.ipen.br/handle/123456789/34052. Acesso em: $DATA.
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ESTEVES-OLIVEIRA, M.; ZEZELL, D.M.
; VELLOSO, W.F.; MEISTER, J.; FRANZEN, R.; LAMPERT, F.; EDUARDO, C.P.; APEL, C..
Low fluence CO2 laser (10.6 μm) parameters for caries prevention.
Caries Research,
v. 41,
n. 4,
p. 325-325,
2007.
DOI:
10.1159/000103637
Abstract:
Although CO 2 laser irradiation can decrease enamel demineralization,
thermal damage to the surface is a common side effect.
The occurrence of fissures and cracks may compromise in
vivo application. Therefore, the aim of the present study was to
find CO 2 laser (10.6 μm) parameters resulting in maximum caries-
preventive effect with the lowest thermal damage. Five low
fluences of 0.12, 0.29, 0.39, 0.50 and 0.60 J/cm 2 combined with
high repetition rates of 500, 154, 167, 182, 187 Hz, respectively and
10 μs pulse duration were chosen for the experiments. 78 bovine
enamel cubes were divided into 5 laser groups and one control.
After treatment the samples were submitted to an 8-day pH-cycling
regime. Demineralization was assessed by lesion depth measurements
with a polarized-light microscope. The temperature
rise at the enamel surface and the propagation into deeper layers
were calculated using a finite element model. Surface morphology
was evaluated by SEM. All laser groups resulted in statistically
significant lower lesion depths than the control group (ANOVA;
p < 0.05). Morphologically, the two lowest fluences resulted in no
surface changes. The calculated temperature rise at 1.5 mm depth
was less than 5 ° C in all groups. In the present in vitro study irradiation
with 0.12 J/cm 2 , 500 Hz and 2500 pulses of CO 2 laser
increased enamel caries resistance without causing undesirable
surface damage or excessive temperature rise.
ESTEVES-OLIVEIRA, M.; ZEZELL, D.M.; VELLOSO, W.F.; MEISTER, J.; FRANZEN, R.; LAMPERT, F.; EDUARDO, C.P.; APEL, C.
Low fluence CO2 laser (10.6 μm) parameters for caries prevention.
Caries Research,
v. 41,
n. 4,
p. 325-325,
2007.
DOI:
10.1159/000103637.
Disponível em: http://repositorio.ipen.br/handle/123456789/34051. Acesso em: $DATA.
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WETTER, NIKLAUS
; FREJLICH, JAIME.
Editorial. Selected Papers from RIAO/OPTILAS 2007 (Campinas, São Paulo, Brazil, 21-26 October 2007).
Journal of Optics A: Pure and Applied Optics,
v. 10,
n. 10,
p. 1-1,
2008.
DOI:
10.1088/1464-4258/10/10/100201
WETTER, NIKLAUS; FREJLICH, JAIME.
Editorial. Selected Papers from RIAO/OPTILAS 2007 (Campinas, São Paulo, Brazil, 21-26 October 2007).
Journal of Optics A: Pure and Applied Optics,
v. 10,
n. 10,
p. 1-1,
2008.
DOI:
10.1088/1464-4258/10/10/100201.
Disponível em: http://repositorio.ipen.br/handle/123456789/34041. Acesso em: $DATA.
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AMANCIO, CARLOS T.; JAKUTIS NETO, JONAS
; WETTER, NIKLAUS U.
; BONFIM JÚNIOR, FRANCISCO A.
; KASSAB, LUCIANA R.P..
Estudo do mecanismo de conversão ascendente no visível em vidros de telureto codopados com Er3+ e Yb3+.
Boletim Técnico da Faculdade de Tecnologia de São Paulo,
v. 23,
2007.
AMANCIO, CARLOS T.; JAKUTIS NETO, JONAS; WETTER, NIKLAUS U.; BONFIM JÚNIOR, FRANCISCO A.; KASSAB, LUCIANA R.P.
Estudo do mecanismo de conversão ascendente no visível em vidros de telureto codopados com Er3+ e Yb3+.
Boletim Técnico da Faculdade de Tecnologia de São Paulo,
v. 23,
2007.
Disponível em: http://repositorio.ipen.br/handle/123456789/34034. Acesso em: $DATA.
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ASSUMPCAO, THIAGO A.A. de; JAKUTIS NETO, JONAS
; WETTER, NIKLAUS U.
; KASSAB, LUCIANA R.P..
Emissão de luz azul em amostras de germanato dopadas com Tm:Yb e Tm:Yb:Nd.
Boletim Técnico da Faculdade de Tecnologia de São Paulo,
v. 21,
2006.
ASSUMPCAO, THIAGO A.A. de; JAKUTIS NETO, JONAS; WETTER, NIKLAUS U.; KASSAB, LUCIANA R.P.
Emissão de luz azul em amostras de germanato dopadas com Tm:Yb e Tm:Yb:Nd.
Boletim Técnico da Faculdade de Tecnologia de São Paulo,
v. 21,
2006.
Disponível em: http://repositorio.ipen.br/handle/123456789/34033. Acesso em: $DATA.
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LIMA, B.L.S. de; COURROL, L.C.; KASSAB, L.R.P.; DEL CACHO, V.D.; GOMES, L.
; WETTER, N.U.
.
Estudo de processos de conversão ascendente em vidros dopados com Nd3+.
Boletim Técnico da Faculdade de Tecnologia de São Paulo,
v. 15,
p. 51-51,
2003.
LIMA, B.L.S. de; COURROL, L.C.; KASSAB, L.R.P.; DEL CACHO, V.D.; GOMES, L.; WETTER, N.U.
Estudo de processos de conversão ascendente em vidros dopados com Nd3+.
Boletim Técnico da Faculdade de Tecnologia de São Paulo,
v. 15,
p. 51-51,
2003.
Disponível em: http://repositorio.ipen.br/handle/123456789/34032. Acesso em: $DATA.
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DEL CACHO, VANESSA D.; KASSAB, LUCIANA R.P.; COURROL, LILIA C.; WETTER, NIKLAUS U.
.
Propriedades espectroscópicas de vidros de fluorborato de chumbo dopados com Nd3+ para uso em laser de estado sólido.
Boletim Técnico da Faculdade de Tecnologia de São Paulo,
v. 13,
p. 27-27,
2002.
DEL CACHO, VANESSA D.; KASSAB, LUCIANA R.P.; COURROL, LILIA C.; WETTER, NIKLAUS U.
Propriedades espectroscópicas de vidros de fluorborato de chumbo dopados com Nd3+ para uso em laser de estado sólido.
Boletim Técnico da Faculdade de Tecnologia de São Paulo,
v. 13,
p. 27-27,
2002.
Disponível em: http://repositorio.ipen.br/handle/123456789/34031. Acesso em: $DATA.
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MONTEIRO, LUCILENA R.
; OLIVEIRA, MERCIA L.; MENEZES, MARIO O.
; POZZO, LORENA
.
Exploratory analysis of a Brazilian real-world open database applied to prostate cancer.
International Journal of Technology Assessment in Health Care,
v. 38,
S1,
p. S97-S98,
2022.
DOI:
10.1017/S0266462322002835
Abstract:
Introduction. Prostate cancer was the second most frequent cancer
and the fifth leading cause of cancer death among men in 2020. The
incidence rates vary substantially in countries with different Human
Development Indexes (HDI), while the mortality rates decrease with
improved access to the health system, availability of therapies and
earlier detection. Worldwide, population-based cancer registries are
important tools for planning and managing health systems. The
Fundação Oncocentro de São Paulo (FOSP) is responsible to collect,
clean and publicize data from cancer treatment institutions. This
study aimed to describe retrospectively the demographic and clinical
profile of prostate cancer (PC) in Brazil using this database. It is not
an incidence study as data is representative only from specific institutions.
Methods. This was a retrospective observational study of the years
2000 to 2020 from analysis of the publicly available FOSP database
(http://www.fosp.saude.sp.gov.br).
The records were extracted, merged, and cleaned using a fully documented
and validated data process. Only patients included on the
register with a primary PC diagnosis were considered.
Results. From January 2010 to June 2020, there were 943,660 patients
diagnosed with C61 in FOSP database for the considered time period.
The majority of the FOSP database records are from patients who live
and/or were born in SP (91.8 and 58.4%, respectively) orMG(2.8 and
10.5%, respectively). The mean age of PC at baseline was 69 years.
Considering the stage of the disease, the mean ages are 55, 70,
67, 66 and 61, for stages 0, 1, 2, 3 and 4, respectively. This cohort
was also analyzed in relation to treatments received, and status at the
end of treatment (51.3% are disease-free, 18.4% are alive with cancer,
and 30.3% are dead). Conclusions. FOSP population-based cancer registries are a powerful
tool to obtain information for planning, and improving the
management of healthcare services especially for São Paulo.
MONTEIRO, LUCILENA R.; OLIVEIRA, MERCIA L.; MENEZES, MARIO O.; POZZO, LORENA.
Exploratory analysis of a Brazilian real-world open database applied to prostate cancer.
International Journal of Technology Assessment in Health Care,
v. 38,
p. S97-S98,
2022.
S1,
DOI:
10.1017/S0266462322002835.
Disponível em: http://repositorio.ipen.br/handle/123456789/33869. Acesso em: $DATA.
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ARAUJO, JOAO V.
; MILAGRE, MARIANA X.
; GABBARDO, ALINE D.
; KLUMPP, RAFAEL E.
; COSTA, ISOLDA
.
The effect of tartaric-sulfuric acid (TSA) anodizing on the corrosion resistance of the AA7475-T761.
ECS Meeting Abstracts,
v. MA2022-02,
2022.
DOI:
10.1149/MA2022-02142475mtgabs
Abstract:
The microstructure of the high strength aluminum alloy, AA7475–T761, in the as-received condition was characterized by optical microscopy, scanning electron microscopy and transmission electron microscopy, and statistical analyses of grain size and micrometer precipitates distribution and density were performed. An anodic aluminum oxide (AAO) film was potentiostatically grown on the alloy in tartaric sulfuric acid (TSA). The anodizing behavior of the alloy and the mechanisms of localized corrosion initiation and propagation in bare (not anodized) and anodized conditions were discussed. The alloy corrosion behavior was investigated in sodium chloride solutions using electrochemical techniques (cyclic potentiodynamic polarization) and the morphology and extent of corrosion propagation was investigated through optical microscopy, optical profilometry and scanning electron microscopy analyses. It was observed that the two alloy surface conditions (bare and anodized) play different roles in the propagation of the localized corrosion process. The bare alloy presented lower localized corrosion potentials and the propagation of the localized corrosion was shallower, but more heavily distributed, than in the anodized condition. However, the alloy in the anodized condition presented lower repassivation potentials due to the deeper pits formed. In addition, the variability of pitting potentials for the anodized condition was high, due to the heterogenous structure of the AAO film formed, and dependent on the time and drying storage conditions previous to electrochemical characterizations. The electrochemical results were correlated with the microstructural characteristics of the oxide surface film of the alloy in both conditions, bare (not anodized) and anodized.
ARAUJO, JOAO V.; MILAGRE, MARIANA X.; GABBARDO, ALINE D.; KLUMPP, RAFAEL E.; COSTA, ISOLDA.
The effect of tartaric-sulfuric acid (TSA) anodizing on the corrosion resistance of the AA7475-T761.
ECS Meeting Abstracts,
v. MA2022-02,
2022.
DOI:
10.1149/MA2022-02142475mtgabs.
Disponível em: http://repositorio.ipen.br/handle/123456789/33867. Acesso em: $DATA.
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FONTES-OLIVEIRA, Y.R.
; CARAMEL-JUVINO, A.
; AVELINO, S.G.
; ZEZELL, D.M.
.
Protocolos de associação de flúor e laser de alta potência (Nd:YAG) na prevenção da cárie: revisão de literatura.
Brazilian Oral Research,
v. 36,
suppl 1,
p. 346-346,
2022.
Abstract:
Avaliar os protocolos publicados para prevenção da cárie utilizando associação de flúor e laser Nd:YAG.
Foram utilizadas seis bases de dados: Web-of-Science, PubMed, ResearchGate, Google Acadêmico,
LILACS e Scielo. Dos 115 artigos inicialmente coletados, foram excluídas as duplicatas, artigos com
dentes bovinos, artigos sem flúor gel ou que abordavam erosão. Foram selecionados 12 artigos. Sobre
os efeitos do tratamento, os artigos relataram que a irradiação laser promove fusão e re-solidificação
da superfície, tornando-a mais ácido-resistente. Quando associado a fluoretos, este laser aumenta a
superfície de contato para a ação do flúor, potencializando os seus efeitos. Analisando os parâmetros
utilizados na irradiação, a maior parte (50%) usou 0,6W de potência e 84,9 J/cm² de fluência, seguido
pelos valores menores 0,5W e 70,7 J/cm² (16,6%). Estes parâmetros foram considerados seguros em
estudos que avaliam a possibilidade de dano térmico à polpa. Dentre os estudos que compararam os
efeitos da aplicação de flúor prévia ou posteriormente à irradiação laser, apenas 8,3% relataram que o
tratamento sinérgico com laser de Nd:YAG e flúor não foi mais eficaz do que o flúor isolado. Já 33,3%
apresentaram melhores efeitos com a aplicação de flúor depois do laser e 8,3% obtiveram melhores
resultados com aplicação antes do laser. 8,3% concluíram que o resultado independe da ordem.
O protocolo de 0,6W de potência e 84,9 J/cm² de fluência, com aplicação de flúor posterior a irradiação
laser, apresentou resultados satisfatórios na prevenção da cárie para a maioria dos estudos.
FONTES-OLIVEIRA, Y.R.; CARAMEL-JUVINO, A.; AVELINO, S.G.; ZEZELL, D.M.
Protocolos de associação de flúor e laser de alta potência (Nd:YAG) na prevenção da cárie: revisão de literatura.
Brazilian Oral Research,
v. 36,
p. 346-346,
2022.
suppl 1.
Disponível em: http://repositorio.ipen.br/handle/123456789/33574. Acesso em: $DATA.
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-
VARDASCA, I.S.
; REGIS, M.A.
; ZEZELL, D.M.
; FRANCCI, C.
.
O uso de fontes de luz em protocolos clareadores: alteração de cor, temperatura, transmitância e irradiância: estudo in vitro.
Brazilian Oral Research,
v. 36,
suppl 1,
p. 306-306,
2022.
Abstract:
O objetivo do presente estudo foi avaliar as alterações de cor superficial, temperatura, transmitância
e irradiância de diferentes fontes de luz em alguns protocolos de clareamento em consultório com
peróxido de hidrogênio (PH) de baixa concentração. 90 blocos de esmalte/dentina humanos foram
pigmentados e separados em 9 grupos(n=10): SG-SL (sem gel-sem luz), SG-VIO (sem gel, com exposição
ao LED violeta), PH35%-SL (sem luz), PH17,5%-SL, PH6%-SL, PH17,5%-AZUL (com exposição ao
LED azul), PH6%-AZUL, PH%17,5-VIO (exposição ao LED violeta) e PH6%-VIO. A avaliação da
cor foi realizada 7, 14 e 21 dias após o início e 14 dias após o término do protocolo de clareamento
com espectrofotômetro, com os valores L*a*b* de reflectância, através do whiteness index (ΔWID
). A análise da temperatura foi realizada com um termopar, a transmitância das luzes com uma
esfera integradora e a irradiância das luzes com um powermeter. ANOVA com post-hoc Tukey para
as análises de alteração de cor, temperatura e transmitância. O teste de Mann-Whitney foi utilizado
para a irradiância. Como resultado, os grupos PH17,5 apresentaram (ΔWID ) similar ao PH35%,
independente das fontes de luz. Todos os protocolos irradiados com fontes de luz apresentaram
alteração de temperatura maior de 5,5ºC, exceto SG-VIO. A transmitância foi maior no LED azul e no
PH17,5%, e a irradiância foi maior no LED azul.
Conclui-se que o uso de fontes de luz associadas a géis clareadores de menor concentração não
melhoram o resultado estético, porém há um aumento considerável da temperatura, maior no LED
Azul que no violeta.
VARDASCA, I.S.; REGIS, M.A.; ZEZELL, D.M.; FRANCCI, C.
O uso de fontes de luz em protocolos clareadores: alteração de cor, temperatura, transmitância e irradiância: estudo in vitro.
Brazilian Oral Research,
v. 36,
p. 306-306,
2022.
suppl 1.
Disponível em: http://repositorio.ipen.br/handle/123456789/33573. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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CARAMEL-JUVINO, A.
; FONTES-OLIVEIRA, Y.R.
; AVELINO, S.G.
; ZEZELL, D.M.
.
Avaliação da desmineralização superficial do esmalte tratado com flúor e laser utilizando Microscopia Eletrônica de Varredura.
Brazilian Oral Research,
v. 36,
suppl 1,
p. 297-297,
2022.
Abstract:
Avaliar o efeito da aplicação tópica de flúor (12.300 μF-/g) associada a irradiação com laser de Nd:YAG
(84 J/cm2) na desmineralização superficial do esmalte, utilizando imagens de Microscopia Eletrônica
de Varredura (MEV). Utilizou-se 60 amostras de esmalte humano (CAAE: 02854118.3.0000.0075),
divididas em 4 grupos: Controle Negativo, Flúor, Laser e Flúor + Laser (n=15). Após os tratamentos,
os grupos foram subdivididos em 3 (n=5), para ciclagem de pH in vitro simulando três condições
diferentes: pH 5 (abaixo do pH crítico do esmalte), pH 4,5 (abaixo do pH crítico do esmalte na
presença do flúor) e pH 4 (investigativo). Quatro imagens por amostras foram obtidas por MEV a 15
kV, em aumento de 4.000X. As imagens foram classificadas como esmalte hígido, desmineralização
leve, moderada ou severa. Para análise estatística, realizou-se uma normalização, onde a classificação
de desmineralização severa foi equiparada a 100% de desmineralização, 0 foi equiparado à classificação
saudável e as demais classificações foram proporcionais. Em pH 5, os grupos Flúor, Laser e Flúor +
Laser não tiveram diferenças significativas entre si (p>0,05). Em pH 4,5, o grupo Flúor + Laser teve o
menor percentual de desmineralização (p<0,05). Já em pH 4, não houve diferença significativa entre
nenhum grupo.
Os dados sugerem que o tratamento de Flúor + Laser tem ação preventiva mesmo em pH abaixo do
crítico para esmalte na presença do flúor (pH 4,5). Além disso, indica que possivelmente este tratamento
altera o pH crítico da estrutura para valores entre 4,5 e 4, ampliando o poder preventivo do flúor.
CARAMEL-JUVINO, A.; FONTES-OLIVEIRA, Y.R.; AVELINO, S.G.; ZEZELL, D.M.
Avaliação da desmineralização superficial do esmalte tratado com flúor e laser utilizando Microscopia Eletrônica de Varredura.
Brazilian Oral Research,
v. 36,
p. 297-297,
2022.
suppl 1.
Disponível em: http://repositorio.ipen.br/handle/123456789/33572. Acesso em: $DATA.
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-
MENDES, V.
; AZEVEDO, L.H.
; ZEZELL, D.M.
.
High-intensity non-ablative lasers in sleep-disordered breathing.
Brazilian Oral Research,
v. 36,
suppl 1,
p. 245-245,
2022.
Abstract:
Primary snoring and severe apnea are considered sleep-disordered breathing and represent different
degrees of severity of the same disorder which affects around one billion people worldwide. It is more
common in men and the elderly. The noise is caused by the soft tissues's vibration in the regions of
greatest constriction of the upper airways. Increased cardiovascular risk, emergence of chronic diseases
and use of medications are due to the progression and evolution of the disease. After determining
optimal irradiation conditions, this study aimed to evaluate the treatment of snoring with sequential
high-intensity non-ablative irradiation with two pulsed lasers Nd:YAG and Er:YAG comparing the
results before and after treatment. A randomized, double-blind, controlled clinical trial is underway
with the approval of the research ethics committee. To date, 32 of 40 volunteers transitioning between
primary snoring and moderate obstructive sleep apnea have been treated in three sessions of nonablative
laser irradiation, 14 days apart. The volume of the oropharyngeal space for air passage was
analyzed by photographic record according to the modified Mallampati index. Oxygen saturation,
snoring severity and sleep quality will also be evaluated. The primary clinical outcome obtained was
the expansion of the upper airway lumen by reducing tissue compliance and, therefore, reducing or
eliminating snoring, in an outpatient procedure.
The reduction of snoring is due to the decrease in tissue compliance.
MENDES, V.; AZEVEDO, L.H.; ZEZELL, D.M.
High-intensity non-ablative lasers in sleep-disordered breathing.
Brazilian Oral Research,
v. 36,
p. 245-245,
2022.
suppl 1.
Disponível em: http://repositorio.ipen.br/handle/123456789/33571. Acesso em: $DATA.
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SARDO, A.V.N.
; CORRÊA, L.
; PRADO, F.C.R.
; FIGUEIREDO, A.
; ANDRADE, M.F.
; ZEZELL, D.M.
.
Avaliação da área epitelial marcada com CK 10 e CK 14 após fotobiomodulação em mucosite oral induzida por radiação gama.
Brazilian Oral Research,
v. 36,
suppl 1,
p. 143-143,
2022.
Abstract:
A mucosite oral (MO) decorre de tratamento quimoterápico e/ou radioterapico em cabeça e pescoço,
podendo gerar úlceras que predispõem o paciente a quadros infecciosos e álgicos. A fotobiomodulação
(PBM) com laser de baixa intensidade vem sendo destaque nos últimos anos, com bons resultados
clínicos. Poucos trabalhos descrevem a ação da PBM na MO em relação ao amadurecimento
epitelial. As citoqueratinas 10 e 14 (CK10 e CK14) são exemplos de proteínas que podem auxiliar
este raciocínio, sendo a CK 10 característica de queratinócitos com maior grau de diferenciação e a
CK14 de queratinócitos em camadas menos diferenciadas. Este trabalho avaliou a extensão da área
epitelial marcada por meio de imunohistoquímica, positiva para CK10 e CK14, em MO radioinduzida
em língua de ratos e tratada por PBM com laser 660 nm e 789 nm, comparados com grupo não
tratado. As amostras foram coletadas nos dias 8 e 20 após a sessão de irradiação. A área expressando
CK10 foi estatisticamente maior no grupo tratado com laser vermelho no vigésimo dia do experimento
em relação aos demais grupos. Para a CK 14, não houve diferença estatística na quantidade de área
marcada, mas sim na distribuição da marcação desta proteína pelo epitélio, que nos grupos tratados
com PBM encontrava-se restrita a camada basal e no grupo controle encontrava-se também marcada
em camadas mais superiores do epitélio.
Conclui-se que a PBM no tratamento de MO com laser 660 nm aumentou a marcação de CK10 e, para
ambos os comprimentos de onda, restringiu a marcação da CK14 a extratos basais de epitélio, como é
esperado em epitélios normais.
SARDO, A.V.N.; CORRÊA, L.; PRADO, F.C.R.; FIGUEIREDO, A.; ANDRADE, M.F.; ZEZELL, D.M.
Avaliação da área epitelial marcada com CK 10 e CK 14 após fotobiomodulação em mucosite oral induzida por radiação gama.
Brazilian Oral Research,
v. 36,
p. 143-143,
2022.
suppl 1.
Disponível em: http://repositorio.ipen.br/handle/123456789/33570. Acesso em: $DATA.
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-
SELLERA, FABIO P.; SABINO, CAETANO P.; NUNEZ, SILVIA C.; RIBEIRO, MARTHA S.
.
Clinical acceptance of antimicrobial photodynamic therapy in the age of WHO global priority pathogens: so what we need to move forward?.
Photodiagnosis and Photodynamic Therapy,
v. 40,
p. 1-3,
2022.
DOI:
10.1016/j.pdpdt.2022.103158
SELLERA, FABIO P.; SABINO, CAETANO P.; NUNEZ, SILVIA C.; RIBEIRO, MARTHA S.
Clinical acceptance of antimicrobial photodynamic therapy in the age of WHO global priority pathogens: so what we need to move forward?.
Photodiagnosis and Photodynamic Therapy,
v. 40,
p. 1-3,
2022.
DOI:
10.1016/j.pdpdt.2022.103158.
Disponível em: http://repositorio.ipen.br/handle/123456789/33456. Acesso em: $DATA.
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PINTO, ANDREIA A.G.; NAGAI, MIRIAN Y. de O.; COIMBRA, EDNAR N.; MOHAMMAD, SUHAM N.
; SILVA, JEFFERSON S.; VON ANCKEN, ADALBERTO; PINTO, SANDRA A.G.; AGUIAR, MICHELLE S.; DUTRA-CORREA, MARISTELA; HORTELLANI, MARCOS A.
; MIRANDA, ADRIANA
; SARKIS, JORGE E. de S.
; SUFFREDINI, IVANA B.; PERES, GIOVANI B.; BERNARDI, MARIA M.; CARTWRIGHT, STEVEN J.; BONAMIN, LEONI V..
Mercury chloride toxicity attenuation of the Brine Shrimp Artemia Salina after treatment with Mercurius corrosivus as isotherapic.
International Journal of High Dilution Research,
v. 21,
n. 2,
p. 6-6,
2022.
DOI:
10.51910/ijhdr.v21i2.1211
Abstract:
Isotherapics prepared from toxic substances have been described as attenuation factors for heavy metal intoxication in aquatic animals. Herein, Artemia salina and mercury chloride were used as a model to identify treatment-related bioresilience. The aim was to describe the effects of Mercurius corrosivus (MC) in different potencies on Artemia salina cyst hatching and on mercury bioavailability. Artemia salina cysts were exposed to 5.0 µg/mL of mercury chloride during the hatching phase. MC 6cH, 30cH, and 200cH were prepared and poured into artificial seawater. Different controls were used (nonchallenged cysts and challenged cysts treated with water, succussed water, and Ethilicum 1cH). Four series of nine experiments were performed for 4 weeks to evaluate the percentage of cyst hatching considering all moon phases. Soluble total mercury (THg) levels and precipitated mercury content were also evaluated. Solvatochromic dyes were used to check for eventual physicochemical markers of MC biological activity. Two-way analysis of variance (ANOVA) with mixed models was used for evaluating the effect of different treatments and the simultaneous influence of the moon phases on the cysts hatching rate, at both observation times (24 and 48 hours). When necessary, outliers were removed, using the Tukey criterion. The level of significance α was set at 5%. Significant delay (p<0.0001) in cyst hatching was observed after treatment with MC 30cH, compared with the controls. An increase in THg concentration in seawater (p<0.0018) and of chlorine/oxygen ratio (p<0.0001) in suspended micro-aggregates was also seen, with possible relation with mercury bioavailability. Specific interaction of MC 30cH with the solvatochromic dye ET33 (p<0.0017) was found. The other observed potencies of Mercurius corrosivus 6 and 200 cH were not significant in relation to the observed groups. The results were postulated as being protective effects of MC 30cH on Artemia salina, by improving its bioresilience.
PINTO, ANDREIA A.G.; NAGAI, MIRIAN Y. de O.; COIMBRA, EDNAR N.; MOHAMMAD, SUHAM N.; SILVA, JEFFERSON S.; VON ANCKEN, ADALBERTO; PINTO, SANDRA A.G.; AGUIAR, MICHELLE S.; DUTRA-CORREA, MARISTELA; HORTELLANI, MARCOS A.; MIRANDA, ADRIANA; SARKIS, JORGE E. de S.; SUFFREDINI, IVANA B.; PERES, GIOVANI B.; BERNARDI, MARIA M.; CARTWRIGHT, STEVEN J.; BONAMIN, LEONI V.
Mercury chloride toxicity attenuation of the Brine Shrimp Artemia Salina after treatment with Mercurius corrosivus as isotherapic.
International Journal of High Dilution Research,
v. 21,
n. 2,
p. 6-6,
2022.
DOI:
10.51910/ijhdr.v21i2.1211.
Disponível em: http://repositorio.ipen.br/handle/123456789/33421. Acesso em: $DATA.
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BIANCOLLI, ANA L.G.
; BARBOSA, ANDREY S.
; KODAMA, YASKO
; REY, JOSE F.Q.; FONSECA, FABIO C.
; SANTIAGO, ELISABETE I.
.
The influence of pre-irradiation and simultaneous grafting methods on the physicochemical properties of polyethylene-based anion-exchange membranes and ionomers.
ECS Meeting Abstracts,
v. MA2022-01,
n. 35,
2022.
DOI:
10.1149/MA2022-01351499mtgabs
Abstract:
Controlling the synthesis parameters during radiation-induced grafting (RIG) is a key feature to design anion-exchange membranes (AEMs) with enhanced properties that lead to high performances and stability. The present study provides a systematic investigation of the effect of RIG methods on physicochemical properties of LDPE-based AEMs with similar degree of grafting (DoG) and ion exchange capacity (IEC). The LDPE films were grafted with 4-vinylbenzyl chloride using two methods: i) pre-irradiation (PIM), using electron-beam (EB), and ii) simultaneous (SM), using gamma-ray (γ), both in air atmosphere and with different absorbed doses. The AEMs grafted by SM were irradiated with 20, 25, and 30 kGy, while the ones grafted by PIM were irradiated with doses of 30, 70, and 100 kGy. Samples grafted by the two different methods have the same molecular structure, but distinct physicochemical properties due to markedly differences in the degree of crosslinking. The studies have shown that grafting step directly influences the internal structure and morphology of AEMs. Multi-technique characterization demonstrated that RIG method determines the mechanical properties, water transport, and the distribution of ionic groups in AEMs. These characteristics have direct impact on fuel cell performance, anion-conductivity, and AEM stability.
Figure 1 shows the polarization curves of anion-exchange membrane fuel cells (AEMFCs) operating at 80 °C with AEMs grafted by PIM and SM. Comparing AEMFCs performances containing AEMs with similar IECs from the two different grafting methods, it is possible to notice that AEMs synthesized by PIM, in general, promote better fuel cell performances than the corresponding ones prepared by SM. The main differences in the polarization curves are evidenced in the ohmic and the mass transport limiting regions. This result suggests that water management is enhanced in AEMs synthesized by PIM compared to the SM-AEMs. A membrane that allows rapid back diffusion of liquid water from the anode to the cathode, should reduce the current losses due to mass transport limitations.
The gel content results confirmed the presence of more crosslinking in membranes synthesized by PIM than in the ones synthesized by the SM. As a consequence of that, enhanced mechanical properties were found for PIM-AEMs, indicating polymer backbone reinforcement due to high degree of crosslinking. Small-angle X-ray scattering measurements have confirmed that the microstructure of PIM and SM AEMs are different and this feature is directly related to ion conduction and water transportation.
Figure 1. AEMFCs performances at 80 °C with LDPE-based AEMs grafted by the pre-irradiation method and by the simultaneous method. H2 anode gas flow=0.8 L min-1, O2 cathode gas flow=0.5 L min-1, both supplied unpressurized with optimal dewpoint temperatures ranging from 77 to 80 °C. AEMs are referred according to the grafting method and absorbed dose. 30 and 70-EB-PIM refer to the samples irradiated with 30 and 70 kGy, respectively, in the EB accelerator and grafted by the pre-irradiation method. 20 and 25-γ-SM refer to AEMs irradiated with 20 and 25 kGy, respectively, by γ-rays and grafted by the simultaneous method.
BIANCOLLI, ANA L.G.; BARBOSA, ANDREY S.; KODAMA, YASKO; REY, JOSE F.Q.; FONSECA, FABIO C.; SANTIAGO, ELISABETE I.
The influence of pre-irradiation and simultaneous grafting methods on the physicochemical properties of polyethylene-based anion-exchange membranes and ionomers.
ECS Meeting Abstracts,
v. MA2022-01,
n. 35,
2022.
DOI:
10.1149/MA2022-01351499mtgabs.
Disponível em: http://repositorio.ipen.br/handle/123456789/33407. Acesso em: $DATA.
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-
ROCHA, R.M.M.
; MENDES, F.M.
; ZEZELL, D.M.
.
Efeitos do filme PVC na performance do DIAGNOdent em lesões de cárie oclusal em dentes decíduos.
Brazilian Oral Research,
v. 18,
Supplement,
p. 240-240,
2004.
Abstract:
O objetivo deste trabalho foi avaliar a influência da utilização do filme PVC, como barreira de proteção, na performance do DIAGNOdent (DD) em lesões de cárie oclusal em dentes decíduos. Foram utilizados 21 molares decíduos que foram avaliados em 5 diferentes condições: (1) DD calibrado sem filme PVC na ponteira, (2) DD calibrado com uma camada de filme PVC e as amostras analisadas, (3) DD calibrado com 3 camadas de filme PVC e as amostras analisadas, (4) DD calibrado sem filme PVC e as amostras mensuradas com o DD com uma camada do filme, (5) DD calibrado sem filme PVC e as amostras mensuradas com o DD com 3 camadas do filme. A validação histológica foi feita por meio de lupa. Foi realizada ANOVA e teste de Tukey para comparação entre os valores obtidos em cada grupo. A área sob a curva ROC para lesões em dentina foi calculada e comparada entre os grupos. Usando o ponto de corte de 13, foi calculada a acurácia (teste de McNemar para comparação). Houve diferença significante entre os valores de medição (p < 0,05) entre os grupos 1 e 5; 1 e 3; 2 e 5. Obteve-se acurácia de 0,81 para os grupos 1, 2 e 3 e de 0,73 e 0,71 para os grupos 4 e 5, respectivamente, mas não houve diferença significante entre esses valores. A área sob a curva ROC mostrou diferença estatisticamente significante entre os grupos 2 e 4.
Concluiu-se que, para melhor desempenho do DD, a ponteira do equipamento deve ser calibrada na mesma condição em que se pretende analisar as lesões. Condições que utilizam 3 camadas de PVC devem ser evitadas, para que não se subestime lesões de cárie em situações clínicas.
ROCHA, R.M.M.; MENDES, F.M.; ZEZELL, D.M.
Efeitos do filme PVC na performance do DIAGNOdent em lesões de cárie oclusal em dentes decíduos.
Brazilian Oral Research,
v. 18,
p. 240-240,
2004.
Supplement.
Disponível em: http://repositorio.ipen.br/handle/123456789/33355. Acesso em: $DATA.
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-
ANA, P.A.
; BLAY, A.
; TURBINO, M.L.
; ZEZELL, D.M.
.
Análise de microdureza de esmalte irradiado com laser Er,Cr:YSGG em baixas fluências.
Brazilian Oral Research,
v. 18,
Supplement,
p. 235-235,
2004.
Abstract:
As modificações nas propriedades químicas do esmalte irradiado provocadas por laser podem indicar o uso deste tratamento como um mecanismo para prevenção da cárie. Este estudo in vitro objetivou avaliar o efeito da irradiação do laser Er,Cr:YSGG em baixas fluências na microdureza do esmalte. Quinze blocos de esmalte foram obtidos de dentes terceiros molares humanos recém-extraídos. As amostras foram embutidas em resina acrílica e foram seccionadas transversalmente em duas porções, sendo uma parte irradiada e outra mantida como controle. Após o polimento, as amostras foram divididas em três grupos e irradiadas de acordo com as seguintes fluências: 2,8 J/cm2 (12,5 mJ/pulso), 5,6 J/cm2 (25 mJ/pulso), e 8,5 J/cm2 (37,5 mJ/pulso). As irradiações foram realizadas com taxa de repetição de 20 Hz, com ausência de spray ar-água e sob a forma de varredura. As superfícies seccionadas das amostras foram submetidas à análise de microdureza Vickers, realizando-se vinte indentações por amostra. Os resultados foram convertidos em porcentagens e submetidos à análise de variância, com p < 0,05. Os resultados mostraram que a microdureza do esmalte dental foi significativamente aumentada nas amostras irradiadas em comparação com as não-irradiadas e as mudanças obtidas foram dependentes das fluências utilizadas.
A irradiação com laser Er,Cr:YSGG em baixas fluências pode aumentar a microdureza superficial do esmalte, o que pode indicar um aumento na sua resistência ácida. Conseqüentemente, o laser Er,Cr:YSGG tem potencial para ser utilizado na prevenção da cárie.
ANA, P.A.; BLAY, A.; TURBINO, M.L.; ZEZELL, D.M.
Análise de microdureza de esmalte irradiado com laser Er,Cr:YSGG em baixas fluências.
Brazilian Oral Research,
v. 18,
p. 235-235,
2004.
Supplement.
Disponível em: http://repositorio.ipen.br/handle/123456789/33354. Acesso em: $DATA.
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-
ANA, P.A.
; ROCHA, R.M.M.
; BLAY, A.
; AUN, C.E.; LAGE-MARQUES, J.L.
; ZEZELL, D.M.
.
Estudo morfológico da superfície de canais radiculares irradiados com laser Er,Cr:YSGG.
Brazilian Oral Research,
v. 18,
Supplement,
p. 164-164,
2004.
Abstract:
O objetivo deste estudo foi avaliar a aparência de canais irradiados com laser de Er,Cr:YSGG após preparo do canal radicular. Selecionou-se nove dentes unirradiculares humanos, cujos canais receberam preparo endodôntico com limas tipo K, associado ao Endo PTC e solução de Dakin. As amostras foram irrigadas com solução desmineralizante EDTA-T por 5 minutos e divididas em 4 grupos. As amostras do grupo I não foram irradiadas, enquanto as do grupo II, III e IV foram irradiadas em associação com a solução irrigadora, utilizando as seguintes fluências: GII (12,5 mJ/pulso; 2,8 J/cm²), GIII (25 mJ/pulso; 8,5 J/cm²) e GIV (75 mJ/pulso; 16,98 J/cm²). A irradiação laser foi realizada com frequência de 20 Hz e ausência de spray ar-água. As superfícies irradiadas foram, então, observadas em microscopia eletrônica de varredura (MEV). Observou-se que as áreas irradiadas apresentaram discreta ablação que aumentava com o aumento da fluência do laser. Não foram encontradas diferenças significativas nos terços coronais das amostras irradiadas ou não irradiadas correspondentes ao padrão de superfície e presença de “smear layer”. No terço médio, as amostras irradiadas apresentaram túbulos abertos e menor quantidade de “smear layer”; no terço apical, as amostras irradiadas exibiram túbulos parcialmente abertos e fina camada de magma dentinário.
Conclui-se que a irradiação de canais radiculares com laser Er,Cr:YSGG remove a “smear layer” e propicia a abertura dos túbulos dentinários, o que pode melhorar a permeabilidade dos canais radiculares e por sua vez a ação de protocolos terapêuticos.
ANA, P.A.; ROCHA, R.M.M.; BLAY, A.; AUN, C.E.; LAGE-MARQUES, J.L.; ZEZELL, D.M.
Estudo morfológico da superfície de canais radiculares irradiados com laser Er,Cr:YSGG.
Brazilian Oral Research,
v. 18,
p. 164-164,
2004.
Supplement.
Disponível em: http://repositorio.ipen.br/handle/123456789/33353. Acesso em: $DATA.
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SILVA, MARIA E. da C.
; RODRIGUES, RENATA B.
; ULIAN, ANDRESSA A..
Revisão das propriedades metabólicas do Bacillus subtilis e suas aplicações biotecnológicas favoráveis à biocenose.
Revista Multidisciplinar em Saúde,
v. 2,
n. 2,
2021.
DOI:
10.51161/rems/1157
Abstract:
Introdução: O gênero Bacillus é considerado um grupo altamente heterogêneo de bactérias, catalogadas em 268 diferentes tipos de espécies e 7 subespécies, tem formato de bastonete e pertence a família Bacilaceae. Ações dessa bactéria não se limitam à promoção da saúde humana, se estendem a macro benefícios no ecossistema e, a consolida como ferramenta no combate ao desequilíbrio ambiental. Mediante os expressivos benefícios, algumas espécies se tornaram foco de relevantes estudos, como o Bacillus subtilis. Essa espécie possui características categóricas, como, pertencem as rizobactérias Gram-positivas e promotoras de crescimento de plantas, termófila, saprófita, produz catalase positiva, não patogênica e capaz de realizar esporulação para manutenção de sua demanda metabólica e inibe a esporulação de fungos. Além disso, sintetiza biossurfactantes e antibióticos com diversas aplicabilidades biotecnológicas, principalmente na biorremediação de solos e água contaminados. O B. subtilis expressa algumas características antimicrobianas, incluindo antifúngicas, e até mesmo nematicidas, esta última característica foi a menos subestimada, considerando as aplicações de tratamento para doenças de nematoides. Enfatizando a produção dos diversos antibióticos, entre eles, lantibióticos e outros, gerados de forma não ribossômica, outras substâncias secundárias, como os compostos policetídeos com propriedade farmacológica, conhecida popularmente por claritromicina e azitromicina. Um exemplo de antibiótico polipeptídico produzido pelo B.subtilis é a bacitracina. Objetivo: Elucidar a ação antimicrobiana e biossurfactantes do B.subtilis, caracterizando-as como propriedades singulares de seu metabolismo que contribuem na síntese de produtos biotecnológicos sem acometer o meio ambiente. Metodologia: Revisão bibliográfica sistemática em sites como: Pubmed, scielo e sciencedirect, utilizando os descritores: “Bacillus subtilis”, “biossurfactantes” e “antimicrobianos”. Resultados: Através da revisão literária foi possível observar um padrão das informações a respeito do Bacillus subtilis que foram tanto como estáveis em suas relações como de suas causalidades, gerando uma nova visão à biotecnologia a favor da vida. Conclusão: Partindo da premissa de que o B.subtilis é uma espécie promotora do equilíbrio da biocenose, essa revisão possibilitou unir informações à nova perspectiva da capacidade promotora do seus metabólitos, principalmente os biossurfactantes e os antimicrobianos que possuem inúmeras aplicabilidades biotecnológica e, a sua coexistência para o fazer o bem à biocenose.
Palavras-Chave:
bacteria;
bacillus subtilis;
biotechnology;
metabolites;
ecosystems
SILVA, MARIA E. da C.; RODRIGUES, RENATA B.; ULIAN, ANDRESSA A.
Revisão das propriedades metabólicas do Bacillus subtilis e suas aplicações biotecnológicas favoráveis à biocenose.
Revista Multidisciplinar em Saúde,
v. 2,
n. 2,
2021.
DOI:
10.51161/rems/1157.
Disponível em: http://repositorio.ipen.br/handle/123456789/33320. Acesso em: $DATA.
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-
SILVA, FABIO A. da
; BERNARDES, EMERSON S.
.
Uncovering the role of modified citrus pectin in cancer.
Nuclear Medicine and Biology,
v. 108-109,
S1,
p. S176-S177,
2022.
DOI:
10.1016/S0969-8051(22)00371-7
Abstract:
Background: Modified citrus pectin (MCP) is a polysaccharide
consisting of galacturonic acid with anti-cancer activity that can act
synergistically with other treatments to reduce tumor growth, stimulate
programmed cell death and reduce the number of metastases.
In addition, MCP prevents acute and severe renal syndromes caused
by radiation/chemotherapy. All of these effects were reported to be
due to MCP ability to specifically inhibit Galectin-3 protein functions.
Aims: The aim of this work was to evaluate the anticancer effect
of MCP in a Balb/c nude mice xenograft model of ovarian cancer.
Methods: The human ovary cancer cell line, SKOV-3, was subcutaneously
injected in Balb/c nude mice and tumor growth was monitored
daily with a caliper. When tumors reached 250-300 mm3, 20
mg/kl of MCP was administered intravenously (I.V.) in a daily based
for 21 days. Tumor growth and mice weight were monitored daily.
Additionally, MCP was radiolabeled with 99m-technetium (99m-Tc)
with the incubation of MCP (2.5 mg) in saline with SnCl2 (4 mg/
ml), HCl (0.01 M), NaOH (0.01 M) and 99mTc (129,5 MBq) at pH=7
for 30 min. The radiochemical purity was determined by iTLC-SG
with acetone and ethanol/NH3/H2O (1:2:5). 99mTc-MCP (37 MBq)
was administrated I.V. in Balb/C nude mice bearing SKOV-3 tumors
and after 1, 2 and 4 hours, SPECT/CT image was acquired (Albira SI
Buker). Ex-vivo biodistribution studies were performed after the I.V.
injection of 10 MBq of 99mTc-MCP for 1-hour. The % of injected dose per gram (%ID/g) of tissues of interest was calculated. The tumors
were removed, fixed, cut and used for autoradiography studies and
stained with hematoxylin and eosin (H&E) to verify hypoxic regions.
Results: The I.V. administration of MCP was able to significantly
reduce SKOV-3 tumor growth (52% tumor volume reduction) in
comparison with the non-treated group, after 21 of treatment. Our
biodistribution studies showed that 99mTc-MCP was mainly found
in kidneys, bladder and liver of mice (%ID/g = 12.25, 38.57 and 5.71,
respectively), and was able to reach the tumor (%ID/g = 0.765 ±
0.045) 1-hour after I.V. administration. 99mTc-MCP accumulation in
the tumor site was visualized by μSPECT/CT imaging 1 hour after I.V.
administration. The autoradiography and stained (H&E) study demonstrated
a correlation between MCP and regions of tumor necrosis.
Because of MCP high accumulation in kidneys, renal toxicity was also
evaluated. We were able to find that MCP doesn’t induce renal toxicity
when administered in a daily base at a concentration of 20 mg/Kg.
Conclusion: In this work we demonstrated that a daily treatment
of MCP was able to reduce tumor growth of ovarian tumor xenografts
(SKOV-3 cells) without showing renal toxicity. We found too that
MCP can reach the tumor site, binding mainly in regions of necrosis.
However, other studies are needed to unravel the mechanisms of
action that act on the antitumor effect of MCP.
SILVA, FABIO A. da; BERNARDES, EMERSON S.
Uncovering the role of modified citrus pectin in cancer.
Nuclear Medicine and Biology,
v. 108-109,
p. S176-S177,
2022.
S1,
DOI:
10.1016/S0969-8051(22)00371-7.
Disponível em: http://repositorio.ipen.br/handle/123456789/33225. Acesso em: $DATA.
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-
GIMILIANI, GIOVANA T.
; IZAR, GABRIEL.
Difficulties in comparison among different microplastic studies: the inconsistency of results and lack of guide values.
Environmental Toxicology and Chemistry,
v. 41,
n. 4,
p. 820-821,
2022.
DOI:
10.1002/etc.5237
GIMILIANI, GIOVANA T.; IZAR, GABRIEL.
Difficulties in comparison among different microplastic studies: the inconsistency of results and lack of guide values.
Environmental Toxicology and Chemistry,
v. 41,
n. 4,
p. 820-821,
2022.
DOI:
10.1002/etc.5237.
Disponível em: http://repositorio.ipen.br/handle/123456789/33037. Acesso em: $DATA.
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-
ZEZELL, DENISE M.
.
Radiation Technology in Health Sciences at IPEN: a multidisciplinary and interdisciplinary Professional Master Degree.
Biophysical Reviews,
v. 13,
n. 6,
p. 1531-1531,
2021.
DOI:
10.1007/s12551-021-00844-3
Abstract:
INTRODUCTION
The Professional Master Program in Radiation Technology
in Health Sciences (MP-TRCS) of the Nuclear and Energy
Research Institute- IPEN/CNEN is a new program, started in
August 2019. It is the only graduation program in the country
to offer two nuclear reactors for educational purposes, for the
development of dissertations, in addition to providing radiopharmaceuticals
production in a nuclear reactor, in linear accelerator
for radioisotope production, as well light and lasers applications. In addition to the infrastructure, the program has multidisciplinary
training advisors working in an interdisciplinary manner
who use their vast experience in radiation applied to medicine
to guide students in a productive manner with a high degree of
excellence.
OBJECTIVES
The MP-TRCS aims to fulfil a growing demand at IPEN/
CNEN from professionals working in hospitals and clinics,
using ionizing and non-ionizing radiation.
MATERIALS AND METHODS
These students need a more dynamic course directed to the
practical professional activities. We have students from the
most diverse areas, such as medical doctors, biomedical
doctors working in clinical analyses, radiotherapy physicists,
physiotherapists, dentists specializing in imaging
diagnosis and laser, among others, participating in the front
line, who use radiation or assess its impact on their day-today
routine.
DISCUSSION AND RESULTS
The first students have already begin to present their dissertation.
The employability has increased among students
enrolled in the program.
CONCLUSION
These professionals bring their experience to the program,
which together with IPEN's academic structure and advisors,
result in skilled students who are finding numerous career
opportunities in the job market.
ZEZELL, DENISE M.
Radiation Technology in Health Sciences at IPEN: a multidisciplinary and interdisciplinary Professional Master Degree.
Biophysical Reviews,
v. 13,
n. 6,
p. 1531-1531,
2021.
DOI:
10.1007/s12551-021-00844-3.
Disponível em: http://repositorio.ipen.br/handle/123456789/32898. Acesso em: $DATA.
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-
VIEIRA, DANIEL P.
.
Three-dimensional cellular culture system for testing of biological effects of radiations in tumoral and non-tumoral models.
Biophysical Reviews,
v. 13,
n. 6,
p. 1521-1521,
2021.
DOI:
10.1007/s12551-021-00844-3
Abstract:
In vitro cell cultures are a well-known controlled test system
used to analyze tumor physiologic responses upon negative
stimuli. Updated techniques, us-ing three-dimensional
organization of cells in cultures, are being increasingly used
to this purpose. Research organizations and industry are
striving to pro-duce in vitro tumor surrogates that could be
better test systems to antitumor agents as new compounds or
to study radiation effects on cancers. The presentation will
show some techniques currently used to build and maintain
these specific cell cultures, and how experiments are
evolving towards the production of tumoroids, or tumoral
organoids, which will include various cell types and additive
manufacturing.
VIEIRA, DANIEL P.
Three-dimensional cellular culture system for testing of biological effects of radiations in tumoral and non-tumoral models.
Biophysical Reviews,
v. 13,
n. 6,
p. 1521-1521,
2021.
DOI:
10.1007/s12551-021-00844-3.
Disponível em: http://repositorio.ipen.br/handle/123456789/32897. Acesso em: $DATA.
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-
DEL MASTRO, NELIDA L.
; GERVASONI, J.L..
Women in the nuclear field promoting Latin American integration.
Biophysical Reviews,
v. 13,
n. 6,
p. 1517-1517,
2021.
DOI:
10.1007/s12551-021-00844-3
Abstract:
Nuclear energy is used for the generation of electricity, but also
for the production of radioisotopes, desalination of sea water
and also for the production of hydrogen. Activities in the nuclear
field are in the area of science, technology and innovation that
has long belonged to an essentially male domain, in which the
contributions of women were neglected or underestimated. The
central idea for the creation of Women in Nuclear, WiN Global,
was to support and encourage women working in nuclear science
and technology and encourage the promotion of understanding
and knowledge of the benefits of the peaceful use of nuclear
energy by the public. WiN Global currently has predominantly
female members coming from 129 different countries, belonging
to chapters or individually. Today, WiN Global is integrated by
53 WiN Global chapters. Forty-nine countries have their own
chapters and there are also regional and international ones. The
history of Latin American integration started during the political
independence movement of the countries of the New Continent.
Since then, up and downs were overcome in order to keep a
regional ambiance of good relationship. In the present study, a
new form of integration is presented by the efforts of the women
working in the nuclear ambit. This important movement involves
Latin American WiN chapters (such as WiN Argentina, WiN
Brazil, WiN ARCAL) promoting activities for the integration of
our region. In order to quantify, to some extent, the participation
of Latin American women, this paper presents a survey crossing
data of the number of related publications to help to address an
objective analysis of the trend of this integration.
DEL MASTRO, NELIDA L.; GERVASONI, J.L.
Women in the nuclear field promoting Latin American integration.
Biophysical Reviews,
v. 13,
n. 6,
p. 1517-1517,
2021.
DOI:
10.1007/s12551-021-00844-3.
Disponível em: http://repositorio.ipen.br/handle/123456789/32896. Acesso em: $DATA.
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YOSHIMURA, TANIA M.
; SAUTER, ISMAEL P.
; RIBEIRO, MARTHA S.
.
Low power light triggers opposite effects on stem cells: influence of the wavelength and culture conditions.
Biophysical Reviews,
v. 13,
n. 6,
p. 1492-1493,
2021.
DOI:
10.1007/s12551-021-00844-3
Abstract:
INTRODUCTION
Photobiomodulation (PBM) has been gaining importance in
a wide range of medical fields in the past few years, particularly
in stem cell-based regenerative medicine. Improving
in vitro cell proliferation, differentiation and viability are
ways where PBM could play a pivotal role optimizing biotechnological
and bioengineering applications.
OBJECTIVES
Here we investigated whether different wavelengths (blue,
green and red) would promote distinct outcomes in human
adipose-derived stem cells (hADSCs) cultured in regular and
supplemented media for tenocyte differentiation.
MATERIALS AND METHODS
Freshly isolated hADSCs were cultured in a specific stem cell
medium (MSCGM, Lonza), DMEM or a tenogenic medium
(TEN-M: DMEM supplemented with growth factors and ascorbic
acid). Cells were irradiated every 48 h (23.28 mW/cm 2 ,
17 min 10 s delivering 24 J/cm 2 per session) using a LED irradiator
(LEDbox, BioLambda). MTT and crystal violet assays
were used to evaluate cell metabolic activity and proliferation.
DISCUSSION AND RESULTS
Red wavelength (660 nm) significantly increased metabolic
activity after five irradiations, but only for cells cultured in
TEN-M. Oppositely, blue (450 nm) and green (520 nm) light
decreased both cell proliferation and metabolic rate, with more
pronounced effects for blue light in TEN-M. Considering these
findings, we examined whether irradiating only the media would generate toxic compounds that could impair cell viability. We
therefore assessed reactive oxygen species (ROS) production
by p-nitrosodimethylaniline/histidine assay while irradiating
the three different media under the same conditions as mentioned
above. Immediately after blue and green light exposure,
an increment in ROS production was observed for DMEM and
TEN-M, that continuously increased until reaching between 4.5
and 7.1 μM one-hour after irradiation – with higher values for
TEN-M exposed to blue light.
CONCLUSION
Since no significant ROS formation was observed following
red light exposure, we concluded that medium composition
was responsible for the different effects on metabolic activity
and proliferation observed after irradiation with different
wavelengths.
YOSHIMURA, TANIA M.; SAUTER, ISMAEL P.; RIBEIRO, MARTHA S.
Low power light triggers opposite effects on stem cells: influence of the wavelength and culture conditions.
Biophysical Reviews,
v. 13,
n. 6,
p. 1492-1493,
2021.
DOI:
10.1007/s12551-021-00844-3.
Disponível em: http://repositorio.ipen.br/handle/123456789/32895. Acesso em: $DATA.
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-
RIBEIRO, MARTHA S.
.
Light-based non-thermal therapy: from basis to clinical applications.
Biophysical Reviews,
v. 13,
n. 6,
p. 1491-1491,
2021.
DOI:
10.1007/s12551-021-00844-3
Abstract:
Light-based non-thermal therapies are evolving as promising
non-invasive and cost-effective medical technologies.
These therapeutic platforms mainly encompass photobiomodulation
(PBM) and photodynamic therapy (PDT), which
use visible or near infrared (NIR) light to induce biological
responses without any significant heating effects. For PBM,
it is most commonly used red or NIR light to optimize light
penetration into biological tissues. The photon absorption by
natural chromophores at these spectral regions cause photophysical
and photochemical reactions inside cells that trigger
several biological effects such as to accelerate wound healing,
reduce inflammation and relief pain, depending on light
parameters and target tissue. On the other hand, PDT makes
use of photoactivated drugs, also called as photosensitizers,
which absorb light to induce chemical reactions that kill
microbial or cancer cells by oxidative stress. Our group have been investigating the mechanisms and several
applications of PBM and antimicrobial PDT (APDT)
for almost 20 years. In this lecture I will share our experience
in the area to discuss how PBM and APDT could be
used to revolutionize health care in the photonics era. An
integrated perspective from the basic mechanisms, preclinical
and clinical trials for both therapies will be presented,
including PBM on cancer management and APDT against
drug-resistant pathogens. The lecture will also highlight
future perspectives.
RIBEIRO, MARTHA S.
Light-based non-thermal therapy: from basis to clinical applications.
Biophysical Reviews,
v. 13,
n. 6,
p. 1491-1491,
2021.
DOI:
10.1007/s12551-021-00844-3.
Disponível em: http://repositorio.ipen.br/handle/123456789/32894. Acesso em: $DATA.
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-
LIMA, MAYELLE M.P.
; NASCIMENTO, PAMELA F. do
; NASCIMENTO, ANA C.G.
; VIEIRA, DANIEL P.
.
A simple and quick method to generate in vitro tridimensional tumor bodies from a human breast adenocarcinoma (MCF7) using magnetic aggregation technique.
Biophysical Reviews,
v. 13,
n. 6,
p. 1470-1470,
2021.
DOI:
10.1007/s12551-021-00845-2
Abstract:
INTRODUCTION
Tumor physiology studies have to rely on efficient and representative
models, as animal-based or in vitro tridimensional cell constructs. The
work used magnetite (Fe3O4) nanoparticles produced by electron-beam
induced chemical reduction to give cells the ability to form aggregates
when submitted to a magnetic field, and thus to produce micro tumors
in vitro.
OBJECTIVES
The work aimed to produce human breast adenocarcinoma mini tumors
(BAMT's) in vitro.
MATERIALS AND METHODS
Paramagnetic iron oxide nanoparticles (PION’s) were synthesized
through electron-beam induced Fe3+ reduction and subsequent
coprecipitation. Due to its poly-L-lysine coating, PION’s were adsorbed
on cell membranes ofMCF7 (human breast adenocarcinoma). Cells were
seeded in 24-well cell culture plates pre-treated overnight with Pluronic®
F-127 to prevent cell adhesion and kept in culture conditions under magnetic
fields for at least 6 days. BAMT's were differentially stained with
Hoescht 33342 and ethidium bromide and imaged by wide-field fluorescence
microscopy.
DISCUSSION AND RESULTS
BAMT's appeared as integer and well-defined cellular aggregates, with
sparse dead cells stained by ethidium bromide. These structures can be
further used for in vitro tumor studies, as BAMT’s are supposed to be
more reliable models than monolayer cultures. Treatment of wells with
poloxamer caused a mild to moderated cell-repellent effect, similar to
those found in commercially available products, only by a fraction of
the cost.
CONCLUSION
The experiments succesfully produced mini tumors prone to be used in
in vitro studies.
LIMA, MAYELLE M.P.; NASCIMENTO, PAMELA F. do; NASCIMENTO, ANA C.G.; VIEIRA, DANIEL P.
A simple and quick method to generate in vitro tridimensional tumor bodies from a human breast adenocarcinoma (MCF7) using magnetic aggregation technique.
Biophysical Reviews,
v. 13,
n. 6,
p. 1470-1470,
2021.
DOI:
10.1007/s12551-021-00845-2.
Disponível em: http://repositorio.ipen.br/handle/123456789/32893. Acesso em: $DATA.
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SANTOS, CAROLINA S.F. dos
; BELLINI, MARIA H.
; SANTOS, SOFIA N. dos
; ARAUJO, ELAINE B. de
.
Estudo sobre a eficiência do radiofármaco PSMA-1007-18F na detecção do câncer de próstata em um estudo pré-clínico in vivo.
Biophysical Reviews,
v. 13,
n. 6,
p. 1467-1467,
2021.
DOI:
10.1007/s12551-021-00845-2
Abstract:
INTRODUCTION
O PSMA-1007-18F é caracterizado por uma biodistribuição única em
comparação com outros agentes de PSMA, pois é eliminado através do
fígado, além de ter captação elevada dentro das células do câncer de
próstata.
OBJECTIVES
O objetivo deste trabalho é analisar a especificidade do radiofármaco
PSMA-1007-18F em camundongos com modelo tumoral PSMA+
através de um estudo de bloqueio.
MATERIALS AND METHODS
Animais SCID com células tumorais LNCaP foram preparados e divididos
em quatro grupos (n=5) de acordo com os tempos de biodistribuição de 30
minutos, uma hora, duas horas e uma hora com agente bloqueador PSMA
I&T (100μg/μL), sendo injetado 30 minutos antes do radiofármaco.
Foram injetados 5,55 MBq (0,056 MBq/μL) via caudal nos animais e
após os tempos pré-determinados foram sacrificados, com os órgãos de
interesse coletados, pesados e sua atividade contabilizada. As imagens
PET/CT foram realizados para ilustrar a captação do PSMA-1007-18F
pelo tumor e por órgãos PSMA+ com e sem bloqueio.
DISCUSSION AND RESULTS
O grupo de 30 minutos apresentou maiores captações, caracterizando o
estágio de distribuição. Rins e baço apresentaram alta captação pelo
PSMA-1007-18F por serem PSMA+, onde sua especificidade pode ser
constata pela drástica diminuição de captação nos animais com agente
bloqueador. A captação pelas células tumorais de próstata se mostrou
constante durante o período avaliado e foi efetivamente bloqueada pelo
excesso de PSMA I&T (imagem), que confirma a ligação do
radiofármaco aos receptores de PSMA. Apesar de apresentar uma baixa
hidrofilicidade, a razão entre tumor/sangue e tumor/músculo foi de 14,18
± 3,19 e 4,78 ± 1,52 em 1h, respectivamente.
CONCLUSION
O radiofármaco PSMA-1007-18F apresenta propriedades de ligação que
fornecem captação tumoral específica. Sua viabilidade em imagens para
câncer de próstata foi demonstrada pela alta especificidade pelas células
PSMA+ e pela via de excreção hepatobiliar. Os resultados deste estudo
serão úteis para subsidiar o registro e comercialização deste radiofármaco.
SANTOS, CAROLINA S.F. dos; BELLINI, MARIA H.; SANTOS, SOFIA N. dos; ARAUJO, ELAINE B. de.
Estudo sobre a eficiência do radiofármaco PSMA-1007-18F na detecção do câncer de próstata em um estudo pré-clínico in vivo.
Biophysical Reviews,
v. 13,
n. 6,
p. 1467-1467,
2021.
DOI:
10.1007/s12551-021-00845-2.
Disponível em: http://repositorio.ipen.br/handle/123456789/32892. Acesso em: $DATA.
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NASCIMENTO, ANA C.G.
; GALISTEO JUNIOR, ANDRES J.; SILVA, GIOVANA D. da
; ROCHA, LEONARDO W.P. de S.
; VIEIRA, DANIEL P.
.
Effective methodology for maintaining Toxoplasma gondii in vitro using paramagnetic iron nanoparticles to support three-dimensional cell culture.
Biophysical Reviews,
v. 13,
n. 6,
p. 1466-1466,
2021.
DOI:
10.1007/s12551-021-00845-2
Abstract:
INTRODUCTION
Toxoplasma gondii is a protozoan parasite that infects approximately one
billion people worldwide. Upon infection, the host may die due to latent
infection or presence with chronic cysts in brain, retina or muscle tissue.
Humans can become infected consuming water or foods contaminated
with oocysts or eating undercooked meat. Its virulent form is difficult to
replicate in vitro, requiring additional steps using experimental animals.
The use of nanotechnology can contribute to this in vitro production,
through the three-dimensional cultivation of mouse fibroblast cells
(NIH / 3T3 ATCC ® CRL-1658™) and nanoparticles synthesized with
radiation.
OBJECTIVES
The objective of this work was to demonstrate the three-dimensional
culture of fibroblast cells aggregated to nanoparticles for inoculation the
T. gondii.
MATERIALS AND METHODS
This methodology was created to facilitate parasite management and replication.
For the production of nanoparticles, the work used concentrations
of iron sulfate II heptahydrate (Fe2SO4.7H2O, CAS 7782-63-0) and
glycine (NH2CH2COOH, CAS 56-40-6) diluted in ultrapure water free ofO2 at pH 12. This solutionwas irradiated by electron beam of the IPEN
/ CNEN-SP Radiation Technology Center in doses of at least 15 and at
most 30kGy. Paramagnetic iron oxide nanoparticles (PION’s) were then
adsorbed on cell membranes, and cells were kept together by a magnetic
field. Structured spheroids (4 day of culture) were infected with 106
parasites (RH strain) and the infection was evaluated by transmission
electron microscopy.
DISCUSSION AND RESULTS
Tachyzoiteswere found inside 3T3 cells, assuring that the spheroid can be
a suitable culture substrate to T. gondii in vitro propagation.
CONCLUSION
A three-dimensionalmethodology for in vitro cultivation of the parasite is
perhaps the key for applications in the study of toxoplasmosis, as it has a
fast, cheap, efficient production (yield and reduction of contamination).
NASCIMENTO, ANA C.G.; GALISTEO JUNIOR, ANDRES J.; SILVA, GIOVANA D. da; ROCHA, LEONARDO W.P. de S.; VIEIRA, DANIEL P.
Effective methodology for maintaining Toxoplasma gondii in vitro using paramagnetic iron nanoparticles to support three-dimensional cell culture.
Biophysical Reviews,
v. 13,
n. 6,
p. 1466-1466,
2021.
DOI:
10.1007/s12551-021-00845-2.
Disponível em: http://repositorio.ipen.br/handle/123456789/32891. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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SEPULVEDA, ANDERSON F.; FRANCO, MARGARETH
; YOKAICHIYA, FABIANO; ARAUJO, DANIELE de.
POLYana: a new software for rheological study of polymeric colloidal materials.
Biophysical Reviews,
v. 13,
n. 6,
p. 1464-1465,
2021.
DOI:
10.1007/s12551-021-00845-2
Abstract:
INTRODUCTION
POLYAna is a new executable software developed by SISLIBIO group for
rheological analysis of hydrogel and organogel systems and other colloidal
materials (nanoparticles and micelles). The software development aims to
facilitate the analysis of rheology data associated to both temperature- and
frequency-dependent analysis, viscosity and curve flow profiles.
OBJECTIVES
The software development aims to facilitate the analysis of rheology data
associated to both temperature- and frequency-dependent analysis, viscosity
and curve flow profiles.
MATERIALS AND METHODS
From raw data, several models are applied like power-law model for
frequency response and curve flow, Boltzmann law to calculate gelation
temperature and viscosity response under temperature,Maxwell model to
study interchain relationships in addition to other models such as
Bingham model, Cross model, and Herschel-Bulkley are also available.
POLYana outputs calculates rheological parameters like consistency, adhesion,
hysteresis, flow index, G’/G” ratio.
DISCUSSION AND RESULTS
To validate results obtained from POLYana, same data were analyzed by
applying other programs and same mathematical models. In this sense, rheological analysis of Poloxamer 407 in water solution (15 %) were
performed: from temperature-dependent G’ and G” analysis were obtained
gelation temperature of 45.46 ± 0.02 °C, η_0 = 0.08 ± 0.03 mPa*s,
η_max = (32.44 ± 0.17) mPa*s and dη/dT = (1.27 ± 0.02) mPa*s/°C by
fitting Boltzmann law (R2 = 0.998), which are similar to results obtained
by others softwares and found in literature. From temperature-dependent
G’ and G” analysis, it gets adhesion value of (1647.15 ± 18.01) mPa*sn
calculated from power-law model (R2 = 0.869), also similar to PRISM
results.
CONCLUSION
Also, other Poloxamer concentrations and hydrogels types have been
evaluated, showing close numbers to that previously reported. In order
to stablish structural relationships, one of POLYana tools is also to analyze
small-angle neutron scattering (SANS) and develop Monte Carlo
simulation for SANS and rheological analysis, simultaneously.
SEPULVEDA, ANDERSON F.; FRANCO, MARGARETH; YOKAICHIYA, FABIANO; ARAUJO, DANIELE de.
POLYana: a new software for rheological study of polymeric colloidal materials.
Biophysical Reviews,
v. 13,
n. 6,
p. 1464-1465,
2021.
DOI:
10.1007/s12551-021-00845-2.
Disponível em: http://repositorio.ipen.br/handle/123456789/32890. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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-
SILVA, CAMILA R.
; PINTO, MAYARA S.
; RIBEIRO, MARTHA S.
.
Photodynamic therapy associated with ionizing radiation in the treatment of triple-negative breast cancer cells.
Biophysical Reviews,
v. 13,
n. 6,
p. 1386-1386,
2021.
DOI:
10.1007/s12551-021-00845-2
Abstract:
INTRODUCTION
Breast cancer is the most common cancer for women worldwide.
According to the World Health Organization, it is considered the 5 th
leading cause of death from cancer. Triple-negative breast cancer
(TNBC) is a subtype of this disease that represents around 20% of all
invasive breast cancer, whose main characteristics are resistance to conventional
treatments, such as exposure to ionizing radiation (IR). On the
order hand, the photodynamic therapy (PDT) using porphyrins and their
derivatives has been described in the literature as a potential therapy
against cancer.
OBJECTIVES
Thus, our goal in this work was to associate PDT and IR in the treatment
of TNBC.
MATERIALS AND METHODS
MDA-MB-231 cells at a concentration of 2x104 cells were submitted to
PDT using TMPyP porphyrin (30 μM) and a red light (660 ±11 nm) with
fluences of the 23 and 57.5 J/cm 2 (57.3 mW/cm 2). Immediately
post-PDT, cells were divided into groups: non-treated (control), only IR
and PDT associated with IR (PDT57+IR and PDT23+IR) and then, exposed
to IR with a dose of 2.5 Gy. Past 24-h of the PDT-session, the cell
viability, clonogenicity and total glutathione were verified.
DISCUSSION AND RESULTS
Cells exposed to IR not presented statistically significance difference
compared to the control group. However, treated groups showed around
38% lower cell viability in relation to the control and IR groups. For the
clonogenic assay a reduction of the approximately 65% was observed
between IR and treated groups. Regarding to the total glutathione, all
groups showed an increase when compared to control group.
Nonetheless, no were identified differences between IR and treated
groups.
CONCLUSION
Taken together, our results indicate that PDTassociate with IR may be an
ally in TNBC treatment.
SILVA, CAMILA R.; PINTO, MAYARA S.; RIBEIRO, MARTHA S.
Photodynamic therapy associated with ionizing radiation in the treatment of triple-negative breast cancer cells.
Biophysical Reviews,
v. 13,
n. 6,
p. 1386-1386,
2021.
DOI:
10.1007/s12551-021-00845-2.
Disponível em: http://repositorio.ipen.br/handle/123456789/32889. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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PINTO, MAYARA S.
; SILVA, CAMILA R.
; SALVEGO, CAMILA de A.
; SIMOES, MARTHA R.
.
Red LED irradiation impacts the cytotoxic response of murine breast cancer cells to ionizing radiation.
Biophysical Reviews,
v. 13,
n. 6,
p. 1385-1385,
2021.
DOI:
10.1007/s12551-021-00845-2
Abstract:
INTRODUCTION
Breast cancer is a disease of worldwide importance since it is considered
the 5th leading cause of cancer deaths. Triple-negative breast cancer
(TNBC) is a molecular subtype that presents resistance to conventional
radiotherapy, demanding high doses of ionizing radiation (IR) for a
prolonged period of treatment. On the other hand, low-level light irradiation
(LLLI) has been studied to sensitize cells before IR exposure.
However, the literature is poor regarding the association of both techniques
in TNBC cells.
OBJECTIVES
Thus, we aimed to assess the effect of LLLI before IR exposure on two
TNBC cell lineages.
MATERIALS AND METHODS
MDA-MB-231 (human TNBC) and 4T1 (murine TNBC) were cultivated,
seeded at a density of 2.5 x 10 5 cells/cm², and maintained in an
incubator (37ºC, 5% of CO2) overnight. LLLI was performed with a
red LED (λ= 660 ± 11 nm, 38.2 mW/cm²) delivering energies of 1.2 J
and 6.0 J. One-h after LLLI, the cells were submitted to both 2.5 and
5.0 Gy doses from a 60 CO source. After 24-h, mitochondrial activity
(MA) was quantified by MTT assay with n= 9/group.
DISCUSSION AND RESULTS
Our data showed that 4T1 cells exposed to LLLI at 1.2 J exhibited higher
MA than cells exposed to IR2.5. In contrast, cells exposed to 6 J of LLLI
showed lower MA than IR5. Concerning MDA-MB231 cells, no statistically
significant differences were noticed among groups regardless of IR
and LLLI doses.
CONCLUSION
These findings indicate that LLLI before IR could sensitize only murine
breast cancer. Besides, an appropriate combination of IR and LLLI doses
seems to play a role to kill TNBC cells.
PINTO, MAYARA S.; SILVA, CAMILA R.; SALVEGO, CAMILA de A.; SIMOES, MARTHA R.
Red LED irradiation impacts the cytotoxic response of murine breast cancer cells to ionizing radiation.
Biophysical Reviews,
v. 13,
n. 6,
p. 1385-1385,
2021.
DOI:
10.1007/s12551-021-00845-2.
Disponível em: http://repositorio.ipen.br/handle/123456789/32888. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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SOUZA, MARESSA D.F. de
; ITRI, ROSANGELA; RIBEIRO, MARTHA S.
.
Reconstitution of Leishmania plasma membrane to understand the photodynamic effect.
Biophysical Reviews,
v. 13,
n. 6,
p. 1384-1384,
2021.
DOI:
10.1007/s12551-021-00845-2
Abstract:
INTRODUCTION
Leishmaniasis is an important neglected disease. Photodynamic therapy
(PDT) has been used to fight cutaneous leishmaniasis showing good
results. However, PDT mechanisms in Leishmania parasites are not yet
completely clarified.
OBJECTIVES
In this work, our objective was to develop a protocol to produce giant
plasma membrane vesicles (GPMVs) from Leishmania amazonensis
promastigotes to understand the mechanisms of action of methylene blue
(MB)-mediated PDT on the cell membrane of parasites.
MATERIALS AND METHODS
For membrane extraction, several techniques were tested. The osmotic
shock was the technique that presented the best yield and effectiveness.
Phosphate and protein measurements were performed to confirm membrane
extraction. For the growth of GPMVs, the best technique was
electroforming using different frequencies and voltages in 4 cycles.
Reconstituted GPMVs were observed by phase-contrast light microscopy.
Subsequently, PDT was applied to GPMVs dispersed in an aqueous
solution containing 50 μM MB and we verified the changes in permeability
before and after exposure to light. The same processwas applied to
giant unilamellar vesicles (GUVs) with lipid compositions similar to the
parasite membrane.
DISCUSSION AND RESULTS
The electroforming technique with the protocol developed in this work
made it possible to obtain GPMVs froma promastigote membrane isolate
of L. amazonensis. The membrane isolation technique was effective to
extract the parasite's membrane while preserving lipids and proteins. In
GUVs we observe an increase in the area during PDT in different compositions
and loss of contrast. The GPMVs showed a loss of contrast as
well as the GUVs but did not show an increase in area.
CONCLUSION
This factor could be explained by the high degree of complexity of the
membrane, which contains membrane proteins in addition to containing
lipids.
SOUZA, MARESSA D.F. de; ITRI, ROSANGELA; RIBEIRO, MARTHA S.
Reconstitution of Leishmania plasma membrane to understand the photodynamic effect.
Biophysical Reviews,
v. 13,
n. 6,
p. 1384-1384,
2021.
DOI:
10.1007/s12551-021-00845-2.
Disponível em: http://repositorio.ipen.br/handle/123456789/32887. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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SOUZA, TIAGO H. dos S.; ANDRADE, CAMILA; CABRAL, FERNANDA
; SARMENTO-NETO, JOSE; REBOUCAS, JULIO; SANTOS, BEATE; RIBEIRO, MARTHA
; FIGUEIREDO, REGINA; FONTES, ADRIANA.
Effects of photodynamic inactivation mediated by Zn(II) porphyrin on promastigote and amastigote forms of Leishmania amazonensis.
Biophysical Reviews,
v. 13,
n. 6,
p. 1380-1381,
2021.
DOI:
10.1007/s12551-021-00845-2
Abstract:
INTRODUCTION
Photodynamic inactivation (PDI) has been attracting attention as an innovative
technology to treat topical diseases, such as cutaneous leishmaniasis
(CL) and infections caused by multidrug-resistant microorganisms.
Zn(II) meso-tetrakis(N-n-hexylpyridinium-2-yl)porphyrin
(ZnTnHex-2-PyP 4+ ) is a lipophilic water-soluble Zn(II) porphyrin with
improved photophysical properties, high chemical stability, and cationic/
amphiphilic character that can enhance its interaction with cells.
OBJECTIVES
Thus, this study aimed to investigate the PDI effects mediated by
ZnTnHex-2-PyP 4+ on Leishmania amazonensis.
MATERIALS AND METHODS
Confocal fluorescence microscopy was explored to study the interaction
of ZnTnHex-2-PyP 4+ with promastigotes. The PDI action was analyzed
by cell membrane integrity, mitochondrial membrane potential (ΔΨm),
and cell morphology. Promastigotes were incubated with
ZnTnHex-2-PyP 4+ for 5 min at 0.62 and 1.25 μM and irradiated by a
LED (410 nm) for 1 or 3 min (2.3 and 3.4 J/cm 2 , respectively). PDI on
amastigotes and the cytotoxicity onmacrophages were also analyzed (3.4
J/cm 2 ).
DISCUSSION AND RESULTS
Fluorescence microscopy revealed that parasites efficiently uptake
ZnTnHex-2-PyP 4+ and displayed a punctate labeling pattern along with
the cytoplasm. An intenseΔΨmdepolarization was also observed, which
in association with microscopy results, suggests that ZnTnHex-2-PyP 4+
may accumulate in the mitochondrion, or other well-defined structures
close to it. Moreover, ZnTnHex-2-PyP 4+ at concentration as low as
0.62 μM led to the immediate inactivation of >95% of promastigotes,
regardless of the light dose used. Loss of the fusiform shape and plasma
membrane wrinkling were also observed. After a single treatment session
in amastigotes, PDI led to a reduction of 70% in the infection index. No
considerable toxicity was observed on mammalian cells.
CONCLUSION
Thus, PDI of Leishmania parasites showed in vitro efficiency at a
submicromolar concentration of ZnTnHex-2-PyP 4+ , with short
pre-incubation and irradiation times. The results encourage further studies
in CL pre-clinical assays and PDI of other microorganisms.
SOUZA, TIAGO H. dos S.; ANDRADE, CAMILA; CABRAL, FERNANDA; SARMENTO-NETO, JOSE; REBOUCAS, JULIO; SANTOS, BEATE; RIBEIRO, MARTHA; FIGUEIREDO, REGINA; FONTES, ADRIANA.
Effects of photodynamic inactivation mediated by Zn(II) porphyrin on promastigote and amastigote forms of Leishmania amazonensis.
Biophysical Reviews,
v. 13,
n. 6,
p. 1380-1381,
2021.
DOI:
10.1007/s12551-021-00845-2.
Disponível em: http://repositorio.ipen.br/handle/123456789/32886. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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-
CONTATORI, CAROLINA G. de S.
; PINTO, MAYARA S.
; RIBEIRO, MARTHA S.
.
Melanoma cell migration in response to red and near-infrared low-level light.
Biophysical Reviews,
v. 13,
n. 6,
p. 1380-1380,
2021.
DOI:
10.1007/s12551-021-00845-2
Abstract:
INTRODUCTION
Cell migration plays an important role in tissue formation and cancer
progression. In vitro scratch assay has been used for many years to study
cell migration to mimic the migration of in vivo cells, and, thus, to evaluate
cancer growth. Low-level red and near-infrared light (LLL) can
increase normal cell migration. However, the impact of LLL on tumor
cells remains unclear.
OBJECTIVES
In this work, we aimed to evaluate the effects of a single LLL dose on
melanoma cell migration.
MATERIALS AND METHODS
B16F10 (murine melanoma) cells were cultivated in RPMI medium with
10% of fetal bovine serum until they reached 80% confluency. The cell
line was seeded in a 6-well plate at a density of 2x10 5 cells/well in
triplicate at two different moments. A wound scratch was performed to
disrupt the confluent cellmonolayerwith a 10 μL pipette tip. Immediately
after the injury, the cells were submitted to the LLL at two distinct wavelengths
(660 and 780 nm) provided by a LED and a laser, respectively,
delivering 3 different energies (1.3, 3.6, and 6 J) at an irradiance of 4.2
mW/cm 2 . The control group was not irradiated. Cells were
photographed immediately and at 3, 12, 24, and 36 h after the scratch.
The wound closure was measured using ImageJ software. To evaluate the
overall migration, we calculated the areas under the curve for each group.
DISCUSSION AND RESULTS
Cells exposed to the red laser at 6 J migrated slower than control. In
contrast, LLL at 780 nm promoted faster cell migration when irradiated
with 3.6 J.
CONCLUSION
These results suggest that low-level LEDs at 660 nm could prevent melanoma
progression in higher energies. However, 780 nm should be
avoided at middle energies.
CONTATORI, CAROLINA G. de S.; PINTO, MAYARA S.; RIBEIRO, MARTHA S.
Melanoma cell migration in response to red and near-infrared low-level light.
Biophysical Reviews,
v. 13,
n. 6,
p. 1380-1380,
2021.
DOI:
10.1007/s12551-021-00845-2.
Disponível em: http://repositorio.ipen.br/handle/123456789/32885. Acesso em: $DATA.
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MITSUTAKE, HERY; SOUZA, ALESSANDRO D. de; YOKAICHIYA, FABIANO; FRANCO, MARGARETH K.K.D.
; CLEMENS, DANIEL; KENT, BEN; BREITKREITZ, MARCIA C.; RIBEIRO, LIGIA N. de M.; PAULA, ENEIDA de; BORDALLO, HELOISA N..
Evaluation of structural changes of benzocaine-loaded, optimized nanostructured lipid carriers using SANS and Raman imaging approaches.
Biophysical Reviews,
v. 13,
n. 6,
p. 1335-1335,
2021.
DOI:
10.1007/s12551-021-00845-2
Abstract:
INTRODUCTION
Local anesthetics are substances that reversibly block the nerve-impulse
conduction, alleviating pain without loss of consciousness. Benzocaine, a
poorly soluble local anesthetic, is an ester of para-aminobenzoic acid.
Several strategies of formulations can be used to improve bioavailability
and decrease adverse effects of benzocaine. In this study nanostructured
lipid carriers (NLC) were employed. These lipid-based drug delivery
carriers have a lipid core composed of a blend of solid and liquid lipids,
and a shelf of non-ionic surfactant.
OBJECTIVES
The main aim of this work was to optimize benzocaine-loaded NLC and
to investigate structural changes in these nanoparticles, under different
temperatures.
MATERIALS AND METHODS
The ratio of excipients (cetyl palmitate, Capmul® PG-8 NF and
Pluronic®F68) and benzocaine in the NLC was optimized using a 2 3
factorial design with respect to the following parameters: particle size,
polydispersity index (PDI) and zeta potentials.
DISCUSSION AND RESULTS
The interactions between the factors were found relevant to determine
particle size and PDI. Using desirability function, the best formulation
conditions were found. Structural changes in optimized NLC were observed
with Small-Angle Neutron Scattering (SANS) and Raman imaging,
in samples at 27, 37 and 40º C. SANS pointed the formation of
lamellar structures inside the NLC, which interlamellar distances increase
at higher temperature. Raman imaging showed that the incorporation of
P68 and benzocaine in-between the lipids increased at higher temperatures,
explaining the changes in Q values (SANS).
CONCLUSION
This work shows how different scattering techniques can provide complementary
information and be used together to characterize and understand
the physical, chemical, and structural changes on the organization
of pharmaceutical carriers in drug delivery system.
MITSUTAKE, HERY; SOUZA, ALESSANDRO D. de; YOKAICHIYA, FABIANO; FRANCO, MARGARETH K.K.D.; CLEMENS, DANIEL; KENT, BEN; BREITKREITZ, MARCIA C.; RIBEIRO, LIGIA N. de M.; PAULA, ENEIDA de; BORDALLO, HELOISA N.
Evaluation of structural changes of benzocaine-loaded, optimized nanostructured lipid carriers using SANS and Raman imaging approaches.
Biophysical Reviews,
v. 13,
n. 6,
p. 1335-1335,
2021.
DOI:
10.1007/s12551-021-00845-2.
Disponível em: http://repositorio.ipen.br/handle/123456789/32884. Acesso em: $DATA.
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SANTOS, CAROLINA M. dos
; SAMPAIO, SUELEN de B.; SANTANA, FAGNER
; LEITE, RODRIGO C.
; PRATA, BEATRIZ A.
; AFFONSO, REGINA
.
A new approach for purification of the catalytic site of the Angiotensin Conversion Enzyme, N domain, mediated by the ELP-Inten system.
Biophysical Reviews,
v. 13,
n. 6,
p. 1305-1305,
2021.
DOI:
10.1007/s12551-021-00845-2
Abstract:
INTRODUCTION
Angiotensin-converting enzyme I, ACE, is a key part of the
renin-angiotensin system whose main function is to regulate blood pressure
and balance of salts in the body. ACE1 has two isoforms, somatic,
sACE, and testicular, tACE. sACE possesses two domains, N- C-, with
catalytic sites which exhibit 60% sequence identity. These domains differ
in terms of chloride-ion activation profiles, rates of peptide hydrolysis and
sensitivities to various inhibitors. N-domain has specific action in the
hydrolyze of Alzheimer’s diseases beta amyloid bodies and angiotensin
1-7, which active the MAS receptor and triggering anti-thrombotic and
anti-inflammatory actions.
OBJECTIVES
The objective this work was to obtain catalytic site Ala361 to Gli468 of
the N-domain region, csACEN, isolation without chromatographic and
denaturant chemical process.
MATERIALS AND METHODS
For that, a new methodology was used in the expression of the csACEN
peptide, in which the peptide was linked to the elastin-like polypeptide,
ELP, and Intein, and expressed at 37C. The characterization of catalytic
site was made by SDS-PAGE and dot blotting.
DISCUSSION AND RESULTS
The culture temperature at 37C significantly increased the expression of
the ELP/Intein/csACEN fusion protein. This culture was lysed at a low
temperature allowing the fusion protein to become soluble. The precipitation
of ELP at high concentrations of ammonium sulfate were obtained
in 0.57 M and 0.8 M. Intein autocleavage occurs at acidic pH and it is
important to pay attention to: pI 6.65 for csACEN and pI 6.87 for
ELPcsACEN, which are very low. The best autocleavage efficiency
was withMES and TriHCl buffers, pH 6.3 and 6.8, respectively, in which
pure csACEn peptide was obtained.
CONCLUSION
The strategy used to obtain the Ala361 to Gli468 catalytic site in soluble
and pure form was obtained with success and the protocol for obtaining
similar peptides was established.
SANTOS, CAROLINA M. dos; SAMPAIO, SUELEN de B.; SANTANA, FAGNER; LEITE, RODRIGO C.; PRATA, BEATRIZ A.; AFFONSO, REGINA.
A new approach for purification of the catalytic site of the Angiotensin Conversion Enzyme, N domain, mediated by the ELP-Inten system.
Biophysical Reviews,
v. 13,
n. 6,
p. 1305-1305,
2021.
DOI:
10.1007/s12551-021-00845-2.
Disponível em: http://repositorio.ipen.br/handle/123456789/32883. Acesso em: $DATA.
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TREMARIN, BEATRIZ; YOKAICHIYA, FABIANO; KELLERMANN, GUINTHER; FRANCO, MARGARETH K.K.D.
; STORSBERG, JOACHIM.
Improvement of the methodological strategies to product functionalizes antibodies using Small Angle Neutron Scattering (SANS).
Biophysical Reviews,
v. 13,
n. 6,
p. 1301-1301,
2021.
DOI:
10.1007/s12551-021-00845-2
Abstract:
INTRODUCTION
Antibodies are used by jawed vertebrates for defense against invading
pathogens. Usage of those versatile tools in a plethora of settings in clinics
and biomedical sciences hinges on functionalization strategies that retain
native antibody reactivity. To this date, antibody functionalization is performed
by trial and error.
OBJECTIVES
We aim to reduce costs by providing general principles to allow the full
spectrum of antibody functionalization by correlating functionalized antibody
reactivity to cognate antigen by small angle neutron scattering,
SANS, measurements and mathematical modeling of antibody and
antibody-antigen super-complexes, obtained by titration experiments.
MATERIALS AND METHODS
For this research we have used for as antibody pure goat anti rabbit
immunoglobulin, and for the antigen, pure Horseradish Peroxidase
Preliminary results show that the systems (antibody and
antibody-antigen complexes) do not change in the range of a temperature
related to storage temperature (25º C), body temperature (37º C) and 40º
C.
DISCUSSION AND RESULTS
These results will give us the pair distribution function of these systems
and the results will be viewed in light of published precedence to highlight
areas where future effort is needed to refine such versatile tools and
improve their production. However, between the antibody and the complexes
structure, different conformations were observed. The antibody
has a globular structure with a radius of gyration around 33 Å, and the
complexes display an elongated cylindrical shape with radius of gyration
around 63 Å.
CONCLUSION
This study shows how the scattering techniques (SANS) can provide
useful information about the conformation of the antibody and
antibody-antigen formation and help to shed light in the understanding
the physical, chemical, and structural changes on the organization of these
important antibody functionalization for the immunological system.
TREMARIN, BEATRIZ; YOKAICHIYA, FABIANO; KELLERMANN, GUINTHER; FRANCO, MARGARETH K.K.D.; STORSBERG, JOACHIM.
Improvement of the methodological strategies to product functionalizes antibodies using Small Angle Neutron Scattering (SANS).
Biophysical Reviews,
v. 13,
n. 6,
p. 1301-1301,
2021.
DOI:
10.1007/s12551-021-00845-2.
Disponível em: http://repositorio.ipen.br/handle/123456789/32882. Acesso em: $DATA.
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-
SUZUKI, L.C.; YAMADA JUNIOR, A.M.
; HAYEK, R.R.A.; RIBEIRO, M.S.
.
A case report of photodynamic therapy on bacterial reduction before immediate implant.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 127-127,
2004.
Abstract:
Recent studies have demonstrated that a number of oral bacteria can
be killed by photodynamic therapy with low concentrations of dyes.
Photodynamic therapy is the combination of light with appropriate
wavelength and a photosensitizer. The antimicrobial activity is mainly
mediated by singlet oxygen and/or free radicals generated by the
photoactivated sensitizer. A flap surgery aiming an immediate
implant was made in a residual root with periodontal lesion on upper
first premolar. After the extraction, a microbiological sample with
sterile paper points was harvested. Then, the photosensitizer was
applied in the infected alveolus and irradiated with low-intensity
laser, ?= 660 nm, P= 30 mW and E= 9 J . After the photodynamic
therapy, a new microbiological sample was harvested. Subsequently,
it was prepared the implant bed with conical burs and then the
implant was placed. Patient was medicated with antibiotic after surgery.
The microbiological analysis showed a significant reduction of
Prevotella sp., Fusobacterium sp. and Streptococcus beta-hemoliticus.
This finding suggests that photodynamic therapy is an alternative
method to disinfect alveolus before implant placement. Therefore,
this study highlights the need for future work in the area of photodynamic
therapy to reduce bacteria without harming host tissue.
SUZUKI, L.C.; YAMADA JUNIOR, A.M.; HAYEK, R.R.A.; RIBEIRO, M.S.
A case report of photodynamic therapy on bacterial reduction before immediate implant.
Brazilian Dental Journal,
v. 15,
p. 127-127,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32767. Acesso em: $DATA.
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NAKASONE, R.K.; RODRIGUES, C.R.M.D.; BUZALAF, M.A.R.; OLYMPO, K.P.K.; TANJI, E.Y.; RIBEIRO, M.S.
.
Effects of the low-intensity red laser radiation on the fluoride uptake in human enamel: a preliminary study.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 119-120,
2004.
Abstract:
Fluoride has been the most important preventive method on development
of the caries. This in vivo study evaluated the effects of lowintensity
red laser radiation on the fluoride uptake in enamel. Ten
healthy participants were recruited for this study. The two upper
central incisors of each volunteer were used and divided into 4
groups: group GC (control), which was untreated; group GF (fluoride),
which received topical acidulated phosphate fluoride (APF)
1,23% treatment for 4 minutes; group GLF (laser + fluoride), which
was irradiated with a low-intensity diode laser (l= 660 nm and dose= 6 J/cm²) with APF application after irradiation and group
GFL (fluoride + laser), which received APF before irradiation using
the same parameters as GLF. The determination of fluoride was
performed using a fluoride ion electrode after an acid-etch enamel
biopsy. The results showed a significant increase of the fluoride
uptake in enamel for groups GF, GLF and GFL when compared to
control group. Although a percentage increase of 57% was
observed for GLF with respect to GF, there were no statistical differences
among the treated groups. These findings motivate further
studies in order to introduce low-power laser associated to APF as
a protective method of dental caries.
NAKASONE, R.K.; RODRIGUES, C.R.M.D.; BUZALAF, M.A.R.; OLYMPO, K.P.K.; TANJI, E.Y.; RIBEIRO, M.S.
Effects of the low-intensity red laser radiation on the fluoride uptake in human enamel: a preliminary study.
Brazilian Dental Journal,
v. 15,
p. 119-120,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32766. Acesso em: $DATA.
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-
MULLER, K.P.
; YAMADA JUNIOR, A.M.
; SUGAYAMA, S.T.; RIBEIRO, M.S.
.
Local Treatment using high and low-power laser on herpes simplex.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 119-119,
2004.
Abstract:
Herpes simplex (HSV) is commonly encountered in the dentistry
practice disappearing after 10 to 14 days. Recently alternative treatments
have been introduced, among those laser therapy. The aim of
this study is to evaluate the efficacy of laser therapy in patients with
perioral herpes simplex infection. A 32 years old female was treated
with high-intensity laser therapy (HILT) in vesicle phase using a
Nd:YAG laser, l= 1064 nm, P= 1,5 W, f= 15 Hz on days 1 and 2 to
drainage the vesicles. Low-intensity laser therapy (LILT) was applied
on the affected area in the 3 consecutive days with a GaAlAs laser, l=
830 nm, D= 5 J/cm2. On the other hand, a 7 years male patient, which
presented the vesiculo-ulcerative stage, only LILT (l= 660 nm, D= 4
J/cm2) was carried out. For both cases, it was observed that the course
of the infection was quicker than the normal. Seven days after treatment
beginning the lesions were healed. These findings suggest that
LILT associated or not to HILT is a efficient and promising treatment
for HSV. Further larger studies aiming at confirming these data and
investigating the LILT mechanisms of action should be performed.
MULLER, K.P.; YAMADA JUNIOR, A.M.; SUGAYAMA, S.T.; RIBEIRO, M.S.
Local Treatment using high and low-power laser on herpes simplex.
Brazilian Dental Journal,
v. 15,
p. 119-119,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32765. Acesso em: $DATA.
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LOLLATO, R.F.; GROTH, E.B.; RIBEIRO, M.S.
.
Low-intensity laser therapy and functional orthopedics in the treatment of pain from temporomandibular disfunction.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 114-114,
2004.
Abstract:
Temporomandibular Disfunction (TMD) is a term used to describe
disorders, which involve temporomandibular joint (TMJ), masticatory
muscles, and associated structures, isolated or not, whose most
frequent symptom is pain. The aim of this study was to evaluate pain
and buccal mobility in subjects with Cl II malocclusion and TMD
symptoms, treated with low-intensity laser therapy (LILT) and functional
orthopedics (FO), associated or not. Eighteen subjects were
selected and divided into three groups. Group 1 was treated with
LILT, l = 780nm, 70 mW, 15 J/cm2 per point during two weeks. The
application was in three points around the TMJ, and trigger points of
masticatory muscles. Group 2 received Planas functional appliances
and it was evaluated once a week during two weeks. Group 3
received both treatments at the same time, and the first laser application
coincided with the appliance installation. Subjects answered
a questionnaire with a score for pain evaluation after palpation.
There was pain relief for the 3 groups and no statistical differences
were observed among them, however, group 3 showed a remission
of pain faster than other groups. This finding indicates the association
of the LILT with FO for pain relief in TMD.
LOLLATO, R.F.; GROTH, E.B.; RIBEIRO, M.S.
Low-intensity laser therapy and functional orthopedics in the treatment of pain from temporomandibular disfunction.
Brazilian Dental Journal,
v. 15,
p. 114-114,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32764. Acesso em: $DATA.
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-
KOHARA, E.K.
; KATO, I.T.; WETTER, N.U.
.
Effects of 960nm diode laser irradiation and fluoride on calcium solubility of dental enamel in vitro.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 112-112,
2004.
Abstract:
The aim of this study is to determine the effects of diode laser
(?=960 nm) irradiation on calcium solubility of dental enamel.
Sixty enamel specimens were prepared from human teeth and
divided into four groups (control, fluoride, laser and fluoride plus
laser). Prior to the irradiation a vegetable coal diluted in physiological
solution was applied to the enamel surface and the specimens
were irradiated using 6,5 W of peak power, 5 ms of pulse
duration, 10 Hz of repetition rate, fluency of 3,7 J/cm2 and time
exposure of 10 seconds. The acidulated phosphate fluoride was
applied for 4 minutes. Next, the specimens were immersed in lactic
acid for 24 hours at 37 °C. The calcium content in demineralization
solution was evaluated with Inductively Coupled Plasma
Atomic Emission Spectrometry. The results indicate an increase in calcium solubility (12%) after laser irradiation, however, not
statistically significant. When associated with fluoride, the
decrease in calcium solubility did not differ from the results
obtained with fluoride only. It can be concluded that in this experiment
diode laser irradiation did not increase acid resistance of
dental enamel.
KOHARA, E.K.; KATO, I.T.; WETTER, N.U.
Effects of 960nm diode laser irradiation and fluoride on calcium solubility of dental enamel in vitro.
Brazilian Dental Journal,
v. 15,
p. 112-112,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32763. Acesso em: $DATA.
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-
KOHARA, E.K.
; WETTER, N.U.
; RATTICHIERI, F.; KATO, I.T..
Microhardness of the dental enamel surface after irradiation with 960nm diode laser.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 111-112,
2004.
Abstract:
Several authors analyzed laser irradiation of dental enamel in an
attempt to increase acid resistance and achieve caries prevention.
In some cases, the procedure has also been associated to the
application of a layer of fluoride. Micro-hardness measurements
are reliable tests used to determine the degree of resistance to
demineralization. In this work micro-hardness values of superficial
dental enamel have been measured before and after irradiation
with a 960 nm diode laser, using tested laser parameters that
are safe and cause no injuries to the adjacent tissue. Laser parameters
were below the dental enamel ablation threshold. 60 enamel
samples were used and divided into control group (GC), fluoride
application (GF), laser irradiation (GL), fluoride application
with subsequent laser irradiation (GFL) and laser with subsequent
fluoride application (GLF). A solution containing coal was
used on the enamel surface in order to absorb the laser radiation.
A statistically significant loss of the micro-hardness value was
obtained for all groups except for GL but also this group did not
produce significant micro-hardness increase. Conclusion: at the
current peak power and beam quality of standard diode laser
bars, safe laser parameters do not produce higher micro-hardness
values.
KOHARA, E.K.; WETTER, N.U.; RATTICHIERI, F.; KATO, I.T.
Microhardness of the dental enamel surface after irradiation with 960nm diode laser.
Brazilian Dental Journal,
v. 15,
p. 111-112,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32762. Acesso em: $DATA.
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CAMARGO, A.; BALDOCHI, S.
; CAMARGO, S.; EDUARDO, C.P..
"In vitro" evaluation of morphologic changes on the root dentine irradiated by CO2 laser associated or not to calcium hydroxide.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 102-103,
2004.
Abstract:
This in vitro study has evaluated the structural changes on the
root dentine irradiated by a CO2 laser associated or not to calcium
hydroxide - an alternative method for dentine hypersensitivity
treatment. Fourteen human teeth 3rd molars were utilized,
divided into 7 groups. Group I (control group),Groups II, III
and IV received an application of Ca (OH)2 paste followed by
CO2 laser irradiation for 5 seconds, intervals of 10 seconds, continuous
emission, power of 0.5W, constant distance of 30mm
(Group II), 1.0W (Group III), 1.5W (Group IV); Groups V, VI,
VII received laser irradiations following the same parameters
applied to groups II, III, and IV. Morphologic changes suggesting
to fusion and re-solidification were observed, and the presence of
material obstructing the whole analyzed surface on groups II, III,
and IV. For groups V, VI, and VII, fusion, re-solidification, and
cracks, were observed, results statistically significant (P<0,01).
No statistically significant differences were observed in groups
that used the same treatment. Powers above 1.0W (DP=125,38W/cm2) showed carbonization and dark surface, results
unfavorable due to the undesirable morphologic alterations and
aesthetic compromising.
CAMARGO, A.; BALDOCHI, S.; CAMARGO, S.; EDUARDO, C.P.
"In vitro" evaluation of morphologic changes on the root dentine irradiated by CO2 laser associated or not to calcium hydroxide.
Brazilian Dental Journal,
v. 15,
p. 102-103,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32761. Acesso em: $DATA.
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-
ARCHILLA, J.R.F.; TANNOUS, J.T.; PELINO, J.E.P.; FREITAS, P.M.; SILVA, D.F.T.; RIBEIRO, M.S.
.
Spectroscopic analysis and interaction of different bleaching agents with 660n laser and 470 nm LED.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 100-100,
2004.
Abstract:
The aim of this in vitro study was to verify the effect of different
dental bleaching methods regarding the achromatic enamel color
change. Forty-five bovine teeth were immersed in a darkening
solution and then divided into nine experimental groups with five
samples in each group. The color measurement was initially
taken using a digital colorimeter. The teeth were submitted to the
bleaching with three bleaching gels, without any activation
source (control) and with two wavelengths (660 nm diode laser
and 470 nm LED). The previous analysis of the absorption spectra
enabled the choice of an adequate wavelength in order to
enhance its photochemical action. The second color measurement
was taken after all the treatments. The data were submitted to
ANOVA and Tukey parametric statistical tests (p=0,05). It was
observed that: 1) there was a statistical significant difference
between the activation sources; 2) there was statistical significant
difference regarding the interaction among the activation sources
and the bleaching gels when compared with each other. The activation
methods of the bleaching agents, even promoting color
changes in distinct levels, still require further studies capable of
detecting a higher absorption spectrum of a product and which
presents significant clinical results.
ARCHILLA, J.R.F.; TANNOUS, J.T.; PELINO, J.E.P.; FREITAS, P.M.; SILVA, D.F.T.; RIBEIRO, M.S.
Spectroscopic analysis and interaction of different bleaching agents with 660n laser and 470 nm LED.
Brazilian Dental Journal,
v. 15,
p. 100-100,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32760. Acesso em: $DATA.
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-
TODESCAN, C.D.R.; VIEIRA JUNIOR, N.D.
; SAMAD, R.E.
; FREITAS, A.Z.
; EDUARDO, C.P..
Ultrashort pulses over bovine dental enamel.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 91-91,
2004.
Abstract:
The interaction of lasers with the hard structures of the teeth, has
found the excess of heat as a problem for its utilization. This
study analyzes, in vitro, the interaction of the ultrashort pulse
laser of Ti:safire (830 nm) with the bovine dental enamel.
The system consisted in one main oscillator integrated with an
amplifier (CPA). The pulses extracted before the temporal compression
inside the amplifier had 30ps, 1000Hz and ~1mJ. The
pulses extracted after the compression had 60fs, 1000Hz and
~0,7mJ. The M2 was 1,3, the focal lens 2,5 cm, the focal distance
29,7 and a computadorized translation stage x,y,z moved the sample.
We evaluated the amount of tissue removed per pulse,the resulting
cavities and the surrounding tissues not irradiated, under OM
and SEM.
The fluency was the major factor for differentiating the two regimens
studied, therefore, the intensity was not so important as we
expected in this process. We found: one ablation region in "cat
tonge", one ablation length, one fluency ~0,7J/cm2 for 30ps and
~0,5J/cm2 for 60fs (50% ofhigh speed burr), smooth edge for
30ps and high precision of the shrp edge cut of submicrometric
order for 60fs.
TODESCAN, C.D.R.; VIEIRA JUNIOR, N.D.; SAMAD, R.E.; FREITAS, A.Z.; EDUARDO, C.P.
Ultrashort pulses over bovine dental enamel.
Brazilian Dental Journal,
v. 15,
p. 91-91,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32759. Acesso em: $DATA.
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-
GODOY, B.M.; ARANA-CHAVEZ, V.E.; BORTOLI GROTH, E.B.; RIBEIRO, M.S.
.
Effects of low-intensity red laser radiation on the dentine-pulp interface after class I cavity preparation disfunction.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 86-87,
2004.
Abstract:
The aim of this study was to investigate the effects of low-intensity
red laser radiation on the ultrastructure of dentine-pulp interface
after conventionally prepared class I cavity preparation.
Eight premolars indicated for extraction for orthodontic reasons
from 2 patients were used. Class I cavities were prepared and the
teeth were divided into two groups. The first group received a
treatment with a GaAlAs laser, l= 660 nm, P= 30 mW and D=
2J/cm2. The laser tip was applied directly and perpendicularly
into the cavity in only one sense. The teeth from the second group
had their class I cavities prepared but they did not receive the
laser therapy. All cavities were filled with composite resin.
Twenty-eight days after the preparation, the teeth were extracted
and processed for transmission electron microscopy analysis.
Two sound teeth (healthy group) without any preparation were
also examined. The first group presented odontoblastic processes in intimate contact with the extracellular matrix, while the collagen
fibers appeared more aggregated and organized than those of
the second group. These results were also observed in the healthy
teeth. The results suggest that laser irradiation accelerates the
recovery of the structures at the dentine-pulp interface involved
during cavity preparation layer.
Palavras-Chave:
teeth;
dentin;
oral cavity;
lasers;
radiation effects
GODOY, B.M.; ARANA-CHAVEZ, V.E.; BORTOLI GROTH, E.B.; RIBEIRO, M.S.
Effects of low-intensity red laser radiation on the dentine-pulp interface after class I cavity preparation disfunction.
Brazilian Dental Journal,
v. 15,
p. 86-87,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32758. Acesso em: $DATA.
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-
NUNEZ, S.C.
; GOMES, L.; GARCEZ, A.S.; LAGE-MARQUES, J.L..
Comparative study between photodynamic therapy and chemical solution on bacterial reduction in root canals.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 85-85,
2004.
Abstract:
One of the major medical problems facing mankind in the next
century will be the resistance of many pathogenic microbes to
existing antibiotics. Oral bacteria can easily reach other body sites
and also spread to other individuals. Therefore, antibiotic-resistant
oral bacteria have the opportunity for rapid dissemination through
the community and to transfer their resistance genes to other bacterial
species. Photodynamic therapy involves the use of light-activated
drugs which may offer an alternative approach to the use of
traditional antimicrobial agents. The purpose of this study was to
evaluate bacterial reduction in infected root canal. Thirty teeth
with their root canals prepared were contaminated with
Enterococcus faecalis. Control group was untreated. Chemical
group was treated with sodium hypochlorite for 30 minutes and in
the laser group, a photosensitizer paste was placed and maintained
in the root canals for 5 minutes and irradiated with a diode laser,
output power 10 mW and l= 685nm for 3 minutes. The bacterial
reduction was significantly higher for laser group when compared to chemical and control groups. These results indicate photodynamic
therapy as an effective method to kill E. faecalis.
Palavras-Chave:
photodynamic therapy;
oral cavity;
teeth;
antimicrobial agents;
microbial drug resistance
NUNEZ, S.C.; GOMES, L.; GARCEZ, A.S.; LAGE-MARQUES, J.L.
Comparative study between photodynamic therapy and chemical solution on bacterial reduction in root canals.
Brazilian Dental Journal,
v. 15,
p. 85-85,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32757. Acesso em: $DATA.
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-
NUNEZ, S.C.
; GOMES, L.; GARCEZ, A.S.; MULLER, K.P.
; JORGE, A.O.C.; RIBEIRO, M.S.
.
Photodynamic action of toluidine blue in streptococcus mutans by fluorescence spectroscopy.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 84-85,
2004.
Abstract:
The antimicrobial activity of toluidine blue associated with red
light has been demonstrated for a wide range of microorganisms
including those commonly found in infected root canals, carious lesions and periodontal pockets. Recent reports have drawn
attention to the problems of antimicrobial resistance and resistance
of oral bacteria to antibiotics and local antiseptics is of
increasing concern, thus photodynamic therapy could be an alternative
antimicrobial approach to treat localized infections in oral
cavity. In this study the fluorescence spectra of TB were obtained
before and after laser exposure in the presence or absence of
Streptococcus mutans. The dye concentration was 0.01%, the
irradiation was performed with a diode laser, l= 660 nm, P=
40mW, exposure time of 3 minutes in a volume of 0.5 ml, with a
pre-irradiation time (PIT) of one or five minutes. The results
showed shifts in fluorescence spectra observed for different preirradiation
times in the presence of S. mutans. In the absence of
bacteria, a shift in the spectra was observed in the dye before and
after irradiation. These findings may indicate a photobleaching
of the dye denoting structural alterations after irradiation and
confirm the importance of the PIT for the success of this therapy.
Palavras-Chave:
dentistry;
caries;
bacteria;
antimicrobial agents;
toluidine blue
NUNEZ, S.C.; GOMES, L.; GARCEZ, A.S.; MULLER, K.P.; JORGE, A.O.C.; RIBEIRO, M.S.
Photodynamic action of toluidine blue in streptococcus mutans by fluorescence spectroscopy.
Brazilian Dental Journal,
v. 15,
p. 84-85,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32756. Acesso em: $DATA.
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-
YAMADA JUNIOR, A.M.
; HAYEK, R.R.A.; GIOSO, M.A.; FERREIRA, J.; BATISTA SOBRINHO, C.A.; RIBEIRO, M.S.
.
Bacterial reduction by photodynamic therapy in peri-implantitis: an in vivo study.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 84-84,
2004.
Abstract:
Progressive peri-implantar bone losses, which are accompanied by
inflammatory process in the soft tissues is referred to as peri-implantitis.
The aim of this study was to compare the effects of lethal photosensitization
with the conventional technique on bacterial reduction
in ligature induced peri-implantitis in dogs. Seventeen third premolars
of eight Labrador dogs were extracted and, immediately after,
the implants were submerged. After osseointegration, peri-implantitis
was induced. After 4 months, ligature were removed and the same
period was waited for natural induction of bacterial plaque. The dogs
were randomly divided into two groups. In the conventional group,
they were treated with the conventional techniques of mucoperiosteal
flaps for scaling the implant surface and irrigation with
chlorexidine. In the laser group, only mucoperiosteal scaling was
carried out before photodynamic therapy. On the peri-implantar
pocket an azulene paste was introduced and a GaAlAs low-power
laser (l= 660 nm, P= 30 mW, E= 5,4 J and Dt= 3 min.) was applied.
Microbiological samples were obtained before and immediately
after treatment. The results of this study showed that Prevotella sp.,
Fusobacterium e S. Beta-haemolyticus were significantly reduced
for the conventional and laser groups (100%,99.8%; 100%,100%;
85.7%,97.6%, respectively).
Palavras-Chave:
dentistry;
teeth;
implants;
bone tissues;
bacteria;
photodynamic therapy;
lasers;
photosensitivity
YAMADA JUNIOR, A.M.; HAYEK, R.R.A.; GIOSO, M.A.; FERREIRA, J.; BATISTA SOBRINHO, C.A.; RIBEIRO, M.S.
Bacterial reduction by photodynamic therapy in peri-implantitis: an in vivo study.
Brazilian Dental Journal,
v. 15,
p. 84-84,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32755. Acesso em: $DATA.
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KOHARA, E.K.
; CRUZ, D.R.; WETTER, N.U.
; RIBEIRO, M.S.
.
Effects of low-intensity laser therapy on the Orthodontic movement velocity of human teeth: a clinical study.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 84-84,
2004.
Abstract:
Low-intensity laser therapy (LILT) has been studied in many
fields of Dentistry, but, to our knowledge, it is the first time that
its effects on orthodontic movement velocity in humans are
investigated. In our study, eleven patients were recruited for a
two-month study. One half of the upper arcade was considered
control group and received mechanical activation of the canine
teeth every thirty days. The opposite half received the same
mechanical activation and was also irradiated with a diode laser
(?=780nm) on ten points around the root, during 10s with 20
mW, 5 J/cm2, on four days of each month. Data of the biometrical
progress of both groups were statistically compared. All
patients showed significant higher retraction velocity of the
canines on the laser treated side when compared to the control.
Conclusion: Our findings suggest that LILT does accelerate human teeth movement and could therefore considerably shorten
the whole treatment duration.
Palavras-Chave:
teeth;
orthodontic treatments;
semiconductor lasers;
light emitting diodes;
therapy;
bone tissues
KOHARA, E.K.; CRUZ, D.R.; WETTER, N.U.; RIBEIRO, M.S.
Effects of low-intensity laser therapy on the Orthodontic movement velocity of human teeth: a clinical study.
Brazilian Dental Journal,
v. 15,
p. 84-84,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32754. Acesso em: $DATA.
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-
BARROSO, M.C.S.; WETTER, N.U.
; PELINO, J.E.P..
Dental bleaching efficacy with diode laser and LED irradiation: an in vitro study.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 77-77,
2004.
Abstract:
Objective: This in vitro study evaluated the whitening efficacy of
LED and diode laser irradiation during the dental bleaching procedure,
using the two agents Opalescence X-tra and HP Whiteness.
Background: Bleaching techniques achieved significant advances
with the use of coherent or incoherent radiation sources to activate the
bleaching chemicals. Methods: Atotal of 60 bovine incisors were randomly
divided into six groups, three for each bleaching agent, receiving
1) only agent, 2) agent and LED irradiation at wavelength of 470
nm, 3) agent and 1.6 watt diode Laser at 808 nm. The results of the
irradiations were characterized with the CIELAB system by measuring
the L*a*b* values for the teeth before and after bleaching.
Results: The average increase of the lightness value (CIELAB L*) of
the different groups was 3-7 and the average chroma value decreased
by 5-9. Conclusions: This is to our knowledge the first time that the
light sources Laser and LED are compared with respect to their
whitening capability when applied to different agents. Best overall
results are obtained with the Whiteness HP and Laser association.
Palavras-Chave:
teeth;
bleaching;
enamels;
semiconductor lasers;
light emitting diodes
BARROSO, M.C.S.; WETTER, N.U.; PELINO, J.E.P.
Dental bleaching efficacy with diode laser and LED irradiation: an in vitro study.
Brazilian Dental Journal,
v. 15,
p. 77-77,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32753. Acesso em: $DATA.
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HAYEK, R.R.A.; YAMADA JUNIOR, A.M.
; GARCEZ, A.S.; NUNEZ, S.C.
; SUZUKI, L.C.; RIBEIRO, M.S.
.
Comparation between photodynamic therapy and a bactericidal solution in the treatment of dental alveolitis microbiological evaluation.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 110-110,
2004.
Abstract:
Photodynamic therapy is a new therapeutic modality to treat
microbial infections. The aim of this pilot study was to compare
this new therapeutic approach with clorhexidine in mini pigs
induced alveolitis. Alveolitis is an inflammatory disease that
involves the superficial bone layer in the dental alveolus. Eight
dental elements were extracted from mini pigs and immediately
after the alveolus were contaminated with Streptoccocus aureus.
After seven days the area presented edema and hyperemia. In the
clorhexidine group the alveolus were debrided and irrigated with a
0.12% solution of clorhexidine. In Laser group the alveolus were
debrided and photodynamic therapy was performed consisting in
the application of a azulene paste inside the alveolus with a preirradiation
time of 5 minutes followed by irradiation with a low
power diode laser, ?= 685 nm, P= 50 mW, E= 9 J, for 3 minutes.
Microbiological samples were harvested before and after treatment
for both groups with sterile paper points. The results showed bacterial
reduction in both groups, although photodynamic therapy
was significantly more effective to reduce the population of S.
aureus. This finding indicates that photodynamic therapy can be an
alternative method to the treatment of alveolitis.
Palavras-Chave:
dentistry;
photodynamic therapy;
germicides;
inflammation
HAYEK, R.R.A.; YAMADA JUNIOR, A.M.; GARCEZ, A.S.; NUNEZ, S.C.; SUZUKI, L.C.; RIBEIRO, M.S.
Comparation between photodynamic therapy and a bactericidal solution in the treatment of dental alveolitis microbiological evaluation.
Brazilian Dental Journal,
v. 15,
p. 110-110,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32752. Acesso em: $DATA.
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-
ZEZELL, D.M.
; BEVILACQUA, F.M.; MAGNANI, R.; ANA, P.A.
; EDUARDO, C.P..
Fluoride incorporation and acid resistance of dental enamel irradiated with Er:YAG: atomic absorption spectrometry and spectrophotometry.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 131-131,
2004.
Abstract:
Er:YAG effects on dental enamel surface regarding the resistance
to demineralization and the fluoride incorporation were
evaluated. 80 samples were divided into 8 groups: G1) control -
APF application; G2) conditioning with 37% phosphoric acid
and APF application; G3) irradiation with 250 mJ/pulse, 7 Hz,
31,84 J/cm2 (contact) and APF application; G4) irradiation with
200 mJ/pulse, 7 Hz, 25,47 J/cm2 (contact) and APF application;
G5) irradiation with 150 mJ/pulse, 7 Hz, 19,10 J/cm2 (contact)
and APF application; G6) irradiation with 250 mJ/pulse, 7 Hz,
2,08 J/cm2 (non-contact) and APF application; G7) irradiation
with 200 mJ/pulse, 7 Hz, 1,8 J/cm2 (non-contact) and APF application;
G8) irradiation with 100 mJ/pulse, 7 Hz, 0,9 J/cm2 (noncontact)
and APF application. All samples were immersed in 2,0
M acetic-acetate acid solution, pH 4,5 for 8 hours. The fluoride,
calcium and phosphorous ions were analyzed, by atomic absorption
spectrometry and spectrophotometry. Groups laser irradiated
before topic APF application presented better results than the
control. There was higher fluoride incorporation on G7 and G8.
Calcium and phosphorous analysis reveled a decrease on the
enamel demineralization on G2 and G3 groups. The Er:YAG
laser on irradiation conditions of this work is a promissory alternative
for the Preventive Dentistry.
Palavras-Chave:
dentistry;
enamels;
lasers;
laser radiation;
fluorides
ZEZELL, D.M.; BEVILACQUA, F.M.; MAGNANI, R.; ANA, P.A.; EDUARDO, C.P.
Fluoride incorporation and acid resistance of dental enamel irradiated with Er:YAG: atomic absorption spectrometry and spectrophotometry.
Brazilian Dental Journal,
v. 15,
p. 131-131,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32751. Acesso em: $DATA.
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-
ZEZELL, D.M.
; MIYAKAWA, W.; RIVA, R.; WATANUKI, J.T..
Monte-Carlo Modelling of light propagation in hard dental tissues.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 131-131,
2004.
Abstract:
In the Monte-Carlo model, we simulated the propagation of visible
light and the temperature distribution in human tooth, which reflects
the energy deposited in the tooth by the laser. As a turbid medium,
differences in absorption and scattering coefficients of the enamel
and dentine must be taken into account. These data are not completely
established in the literature in the visible range. The results
are compared with experimental data of Cu-HyBrID laser light
propagation in human molar teeth, in order to evaluate the scattering
coefficient. The Cu-HyBrID laser emits green (510 nm) and yellow
(578 nm) radiation with high output peak power (20 kW) at high
repetition rates (13.7 kHz) and there is almost no report of its use in
Dentistry. This work aims to correlate the Cu-HyBrID energy deposition
with the tooth thermal response. The tooth is predominantly a
scattering medium (absorption much lower than scattering) and
small variations in the absorption coefficient do not reveal significant
alterations in the light distribution curve. According to the simulation,
most of the laser energy is accumulated on enamel-dentine
junction and the tooth thermal response is strongly affected by the
value of the absorption coefficient, which is not yet precisely known.
Palavras-Chave:
dentin;
teeth;
laser radiation;
monte carlo method;
simulation;
enamels
ZEZELL, D.M.; MIYAKAWA, W.; RIVA, R.; WATANUKI, J.T.
Monte-Carlo Modelling of light propagation in hard dental tissues.
Brazilian Dental Journal,
v. 15,
p. 131-131,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32750. Acesso em: $DATA.
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-
HAYPEK, P.; THEODORO, L.H.; BACHMANN, L.
; EDUARDO, C.P.; SAMPAIO, J.E.C.; ZEZELL, D.M.
.
Effects of the diode laser irradiation on root surfaces: thermal analysis.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 110-110,
2004.
Abstract:
The purpose of this study was to evaluate the temperature rising inside the pulpar cavity during the diode laser irradiation on the
root surface. Twelve single root teeth were used and their mesial
and distal surfaces were irradiated (808 nm, 400 m, 30 s), in a
continuous mode (CW) and in an switched mode (SW) (10 Hz).
The temperature rise was evaluated deppending with the power
used (0.4 W to 2.2 W). The temperature was monitorated with a
termopar (T) fixed inside de pulpar cavity during the irradiation.
In a second step two parameters was used in a SW (10 Hz). The
safety temperature was keep using the power: Group A - 0.9 W
(mesial ) and Group B - 1.08 W (distal). The results showed the
termal pulpar events (T) during the irradiation, based in an specific
relation with the power (P), like T= -0.4+3.7P in a CW and
T= -0.2+2P in a SW. The temperature rising during the irradiation
was 3.4±0.4ºC on Group A and 4.0±1.0ºC on Group B.
The irradiation mode and the power are much important to temperature
rise inside the pulpar cavity and the 0.9 W and 1.08 W
radiation on the root surfaces were safety.
Palavras-Chave:
semiconductor diodes;
laser radiation;
dentin;
temperature dependence;
teeth
HAYPEK, P.; THEODORO, L.H.; BACHMANN, L.; EDUARDO, C.P.; SAMPAIO, J.E.C.; ZEZELL, D.M.
Effects of the diode laser irradiation on root surfaces: thermal analysis.
Brazilian Dental Journal,
v. 15,
p. 110-110,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32749. Acesso em: $DATA.
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FERNANDES, A.S.F.; NAVARRO, R.S.; GONTIJO, I.; HAYPEK, P.; ZEZELL, D.M.
; HADDAD, A.E..
Intrapulpar temperature variation in primary teeth during cavity preparation using: Er:YAG laser and conventional high-speed drill.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 106-107,
2004.
Abstract:
Increases of temperatures superior than 5-5.5°C can compromise
the pulp vitality. The purpose of this in vitro study was to compare the variation of the pulp temperature in primary teeth during
the cavity preparation. Twelve primary lower incisors (Human
Tooth Bank-FOUSP) were divided in 4 groups (n=3): cavity
preparations were performed at buccal surface, refrigerated by
air-water spray, during 20sec, using #1090 diamond burr in highspeed
drill in carious-free incisors (G I) and carious incisors (G
II); or Er:YAG laser (2.94μm)(KaVo 3)(LELO-FOUSP), with
6Hz repetition rate/ 600mJ energy as orientated by manufacturer,
in carious-free incisors (G III) and carious incisors (G IV). The
variation of temperature was measured using digital oscilloscope
and thermocouple type K placed inside the pulpar chamber,
simultaneously the teeth was kept under water thermal bath during
the procedures. The temperatures rises were less than 4ºC in
all cavities preparations in different groups, the variation of temperature
in laser and conventional high-speed drill groups
showed similar values. The groups of carious teeth showed higher
increase of temperature than carious-free teeth. The Er:YAG
laser demonstrated be a safe alternative for cavities preparations
in primary teeth, since that using effective and safety parameters
by qualified and trained professionals.
Palavras-Chave:
teeth;
temperature dependence;
caries
FERNANDES, A.S.F.; NAVARRO, R.S.; GONTIJO, I.; HAYPEK, P.; ZEZELL, D.M.; HADDAD, A.E.
Intrapulpar temperature variation in primary teeth during cavity preparation using: Er:YAG laser and conventional high-speed drill.
Brazilian Dental Journal,
v. 15,
p. 106-107,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32748. Acesso em: $DATA.
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-
EDUARDO, P.L.P.; BACHMANN, L.
; SALVADOR, V.L.; ZEZELL, D.M.
.
Chemical Modification of bovine dental enamel irradiated with holmiun laser.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 106-106,
2004.
Abstract:
This study investigated Ho:YLF (2,065 mm) effects on dental
enamel with regards to the thermal variations in the pulp chamber
during irradiation and resistance to demineralization. Twenty
samples of bovine enamel were divided: 1) control- acidulated
phosphate fluoride (APF) topic application followed by demineralization
treatment with lactic acid; 2) Ho:YLF irradiation
(100J/cm2) followed by APF and demineralization; 3) Ho:YLF
irradiation (350J/cm2) followed by APF and demineralization; 4)
Ho:YLF irradiation (450J/cm2) followed by APF and demineralization.
All samples were quantified according to their calcium
and phosphorous atoms relative concentrations before and after
the treatments. X-Ray fluorescence spectrochemical analysis
showed an increase on the calcium and phosphorous atoms concentration
ratio and therefore the enamel demineralization reduction
as a result of the lactic acid treatment in the samples irradiated with the holmium laser followed by the APF (ANOVA, Tukey
p<0,01). To evaluate the feasibility of this study for clinical purposes,
surfaces morphology were analyzed. Modifications were
characterized by melted and re-solidified regions of the enamel,
which can changes its permeability and solubility. Temperature
changes of ten human pre-molars teeth irradiated with 350 J/cm2
and 450 J/cm2 were also monitored in the pulp chamber in real
time. Temperature increases were limited to 4,2° C.
Palavras-Chave:
teeth;
dentin;
animals;
cattle;
enamels;
laser radiation;
holmium;
chemical composition
EDUARDO, P.L.P.; BACHMANN, L.; SALVADOR, V.L.; ZEZELL, D.M.
Chemical Modification of bovine dental enamel irradiated with holmiun laser.
Brazilian Dental Journal,
v. 15,
p. 106-106,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32747. Acesso em: $DATA.
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-
ANA, P.A.
; VELOSO JUNIOR, W.F.; CLAUDIO, T.; ZEZELL, D.M.
.
3D-Finite element and thermographic analysis of human teeth irradiated with ErCrYSGG.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 99-99,
2004.
Abstract:
The purpose of this study was to analyse surface and pulpal temperature
changes in extracted human teeth subjected to
Er,Cr:YSGG laser irradiation by using Finite Element Method
(FEM) and comparing with thermal analysis made by thermocouple
and thermocamera. Twelve extracted human molar teeth
were selected and divided into three groups, according to the following
Er,Cr:YSGG laser irradiation conditions: 2.8 J/cm2, 5.6
J/cm2 and 8.5 J/cm2. During laser irradiation, surface temperature
was measured using a thermocamera (FLIR Systems - USA)
and the pulpal temperature was monitored by means of a thermocouple
type T placed in the pulpal chamber. For FEM analysis, it
were constructed numerical models that reproduced a typical
sound molar teeth and the analysis simulated the absorption of the
laser energy and the heat transfer through the tooth in three
dimensions, considering the same laser energy and conditions
performed in thermocouple and thermocamera measurements.
The results showed that theoretical FEM calculation showed
good agreement with the thermocamera and thermocouple
obtained data, simulating heat transfer and predicting the temperature
increase. It was possible to conclude that 3D Finite Element
Method gives good results that makes possible to understand the
thermal behavior of dental material.
Palavras-Chave:
teeth;
finite element method;
thermography;
laser radiation
ANA, P.A.; VELOSO JUNIOR, W.F.; CLAUDIO, T.; ZEZELL, D.M.
3D-Finite element and thermographic analysis of human teeth irradiated with ErCrYSGG.
Brazilian Dental Journal,
v. 15,
p. 99-99,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32746. Acesso em: $DATA.
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ANA, P.A.
; ROCHA, R.M.M.; BLAY, A.; AUN, C.E.; LAGE-MARQUES, J.L.; ZEZELL, D.M.
.
Effects of Er, Cr:YSGG Laser irradiation on endodontics system permeability.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 99-99,
2004.
Abstract:
The aim of this study was to evaluate the effects of Er,Cr:YSGG
laser irradiation on root dentin permeability. Twenty extracted
single-rooted teeth were instrumented with K-files and divided in
four groups, according to laser fluences: GI (non-irradiated), GII
(12.5 mJ/pulse; 2.8 J/cm2), GIII (25 mJ/pulse; 8.5 J/cm2) and
GIV (75 mJ/pulse; 16.98 J/cm2). Lased groups had an association
of irrigating solution EDTA-T and Er,Cr:YSGG laser irradiation,
which was performed at 20 Hz repetition rate without airwater
spray, with four helicoidal movements during approximately
5 seconds. NDP associated with rhodamine B dye was
used to evaluate dentin permeability. After the experimental period,
the samples were transversely cut into six 2.0 mm thick slices
for subsequent reading using the ImageLab software. The results
showed that dentin permeability of root canals was decreased
when exposed to Er,Cr:YSGG laser irradiation compared with
non-irradiated samples, and this alteration was higher when higher
fluences were applied. The cervical and middle thirds had permeability
values statistically similar (p > 0.05) and significantly
greater than the apical third. It can be concluded that the use of
Er,Cr:YSGG laser irradiation at the utilized parameters decreases
dentin permeability, favoring the root canal sealing.
Palavras-Chave:
dentin;
laser radiation;
permeability
ANA, P.A.; ROCHA, R.M.M.; BLAY, A.; AUN, C.E.; LAGE-MARQUES, J.L.; ZEZELL, D.M.
Effects of Er, Cr:YSGG Laser irradiation on endodontics system permeability.
Brazilian Dental Journal,
v. 15,
p. 99-99,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32745. Acesso em: $DATA.
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EDUARDO, F.P.; NICOLLI FILHO, W.; MIGLIORATI, C.A.; ZEZELL, D.M.
; EDUARDO, C.P.; SCHUBERT, M.M..
Effects of low intensity laser radiation in the prevention of oral mucositis in patients undergoing bone marrow transplant.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 97-97,
2004.
Abstract:
Oral mucositis is one of the complications arising from pre bone marrow
transplant conditioning, which can substantially change the patient's quality
of life. The purpose of this randomized double blind study was to compare
the effects of low intensity laser radiation in the prevention of oral
mucositis in patients undergoing bone marrow transplants. Seventy
patients at the Seattle Cancer Care Alliance in the U.S.A. were approved
by the local ethics committee and gave their informed consent to take part
in the study. The 70 patients were divided into three groups (group 1 -
laser 650nm; group 2 - laser 780nm and group 3 placebo). The therapy or
placebo treatment began on the first day of the conditioning and continued
through to two days following the bone marrow transplant. Mucositis
was measured according to the oral mucositis rate and the pain assessment
rate (VAS). We were thus able to conclude that the diode 650nm
laser indeed decreased the severity of oral mucositis as well as the degree
the pain when used as a preventative therapy in patients undergoing bone
marrow transplants. In this study, low intensity laser therapy was regarded
as safe and did not present any side effects.
Palavras-Chave:
low dose irradiation;
oral cavity;
mucous membranes;
lasers;
semiconductor diodes;
transplants;
bone marrow
EDUARDO, F.P.; NICOLLI FILHO, W.; MIGLIORATI, C.A.; ZEZELL, D.M.; EDUARDO, C.P.; SCHUBERT, M.M.
Effects of low intensity laser radiation in the prevention of oral mucositis in patients undergoing bone marrow transplant.
Brazilian Dental Journal,
v. 15,
p. 97-97,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32744. Acesso em: $DATA.
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PAIVA, P.F.; PAIVA, G.; NASR, M.K.; NUNES, L.J.; MOREIRA, L.A.; ZEZELL, D.M.
.
Cavity preparation with ER:YAG laser: pain evaluation.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 94-94,
2004.
Abstract:
They were selected for this work clinic patient of the which were
selected 15 teeth with decay lesion, being ten teeth with lesion type
class I, of these five for the group-control with high conventional
rotation, and five for the group laser class I, and five teeth with
lesion type class Vfor the group laser. In the preparations with laser
of Er:YAG (Kavo Key Laser 2), any patient do not was anesthetized,
even in the deepest cavities, and the maximum degree of
pain (that varied from 0 to 10) it was of 4. In the group-control,
with mounted tip in high conventional rotation, two patients were
anesthetized, and the maximum degree of pain was of 7.
The use of the laser in the dental clinic (restorative dentistry),
using the technology laser in the dental preparations, it showed to
be a good alternative to the use of the mounted tip in high conventional
rotation.
94
Palavras-Chave:
dentin;
teeth;
oral cavity;
lasers;
biological recovery;
pain
PAIVA, P.F.; PAIVA, G.; NASR, M.K.; NUNES, L.J.; MOREIRA, L.A.; ZEZELL, D.M.
Cavity preparation with ER:YAG laser: pain evaluation.
Brazilian Dental Journal,
v. 15,
p. 94-94,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32743. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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-
GUIMARAES, J.G.A.; MIYAKAWA, W.; STOLF, S.F.; SILVA, E.M.; ZEZELL, D.M.
; EDUARDO, C.P..
In vitro thermographic measurement in pulpal chamber during diode laser bleaching.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 94-94,
2004.
Abstract:
Thermographic was employed to determine the temperature rise
in lower incisors pulpal chambers during diode laser bleaching.
Two methods were used: a thermocouple for 72 teeth and a
infrared (IR) thermographic camera for 36. Two bleaching
agents, both 35% hydrogen peroxide- Whiteness HP (HP) and Hi
Lite (HL) - were applied to the specimens buccal surfaces and
irradiated with a diode laser (808 5nm), CW for 30s. Intensities
tested were 21.W/cm2, 29.8W/cm2, 35.8W/cm2, 38.2W/cm2,
52.9W/cm2 e 63.7W/cm2. Means of the greatest temperature
rises with the HL were statiscally lower than the HP (p<0.01).
When HP was irradiated with 50.9W/cm² and 61.1W/cm², the
temperature registered was over 5.5ºC, considered as the limit to
avoid pulp damage. The IR thermacam analysis showed that,
when the HP was used, the temperature rise in pulp chamber was
similar to the target area on the buccal surface. Evaluation of
tooth color was done using a VITAshade guide at baseline and at
the end of the bleaching treatment. Both products proved to be
efficient, however HP produced statiscally higher shade changes
than HL (p<0.01). It can be concluded that the diode laser bleaching
associated with the HP was safe when intensities below
50mW/cm2 were employed. Higher parameters can cause damage
to pulp vitality of the lower incisors, fact that did not
occurred with the HL gel. Both gels were efficacious to the
bleaching technique proposed, but the HP showed better results.
Palavras-Chave:
dentin;
teeth;
bleaching;
thermography;
hydrogen peroxide;
semiconductor diodes;
lasers
GUIMARAES, J.G.A.; MIYAKAWA, W.; STOLF, S.F.; SILVA, E.M.; ZEZELL, D.M.; EDUARDO, C.P.
In vitro thermographic measurement in pulpal chamber during diode laser bleaching.
Brazilian Dental Journal,
v. 15,
p. 94-94,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32742. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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-
GONTIJO, I.T.; CIAMPONI, A.L.; ZEZELL, D.M.
; NAVARRO, R.; RODRIGUES, W.; CIAMPONI, A.L..
In vitro and in vivo evaluation of deciduos teeth withening technique: the diode laser and light cure.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 93-93,
2004.
Abstract:
A great number of children suffer from traumatic injuries on the
deciduous dentition. The darkening resulting from these injuries
create an aesthetic probem in these children in the middle of their
psychosocial development. The whitening technique might be a
satisfactory aesthetic resolution, as well as non-invasive. The
objectives of the present study were to evaluate "in vitro" and "in
vivo" the teeth color variation and superficial temperature,
obtained by the thermocatalytic technique used in devitalized
human deciduous teeth, as well as evaluate "in vivo" the teeth
color variation obtained by the whitening. The whitening agent
was the hydrogen peroxide 35%,having as a variant the source of
catalyzing energy- diode laser and the light curer. 21 deciduous
teeth were utilized. The light curer group-11 teeth and the laser
group, 10.The color evaluation was carried out by the spectrophotometer
and VITA 3D scale. After statistic analysis, it can
be concluded that the whitening was verified by both
methods.The temperature variation was significantly higher in
the light cure group than in the laser group.
Palavras-Chave:
dentin;
teeth;
hydrogen peroxide;
semiconductor diodes;
bleaching
GONTIJO, I.T.; CIAMPONI, A.L.; ZEZELL, D.M.; NAVARRO, R.; RODRIGUES, W.; CIAMPONI, A.L.
In vitro and in vivo evaluation of deciduos teeth withening technique: the diode laser and light cure.
Brazilian Dental Journal,
v. 15,
p. 93-93,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32741. Acesso em: $DATA.
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-
ANDRADE, A.K.P.; FEIST, I.S.; CAI, S.; PANNUTI, C.; ZEZELL, D.M.
; DE MICHELI, G..
Bacterial reduction in class II furcation after root debridment with or without Nd:YAG laser irradiation.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 90-91,
2004.
Abstract:
The use of Nd:Yag laser for bacterial reduction as an adjuvant to
nonsurgical periodontal treatment has been approached in several
studies. Furcation complex anatomy is responsible for comprised
treatment results in this areas due to the lack of proper
access for instrumentation showing the persistence of a pathogenic
microbial flora. The purpose of this clinical trial, randomized,
double-blinded was to evaluated the bacterial reduction achieved
with the Nd:YAG associated to conventional treatment on furcation
sites of patients with chronic periodontitis. In a split mouth
design study, 34 class II furcations that were selected from 17
patients with chronic periodontitis. They received previous full
mouth periodontal treatment, except for the experimental sites.
The 17 furcations of the Control group underwent twice manual
and ultrasonic root debridment in weekly intervals. The Test
group received the same treatment as the Control group followed
by the Nd:YAG laser application (100mJ/pulse; 1.5W; 15Hz;
60sec). The microbiological parameters total numbers of anaerobic
Colony Forming Units(CFU); Black pigmented CFU and the
level of Actinobacillus actinomycetemcomitans(Aa), Porphyromonas
gingivalis (Pg) and Prevotella intermedia(Pi) were determined
at baseline, immediatly and one month after the treatment.
The results showed a significant reduction of total CFU for both
groups immediately after the treatment, but it was better for the
Test group. After one month the total CFU average increased but was still below pretreatment levels for both groups. The black
pigmented CFU and the level of Aa, Pg e Pi decreased significantly
after the treatment but 30 days after there was an increase
almost equal to baseline levels for both groups. The Nd:Yag laser
associated with convencional treatment promoted bacterial
reduction on class II furcation immediately after its application.
Palavras-Chave:
bacteria;
laser radiation;
lasers;
clinical trials
ANDRADE, A.K.P.; FEIST, I.S.; CAI, S.; PANNUTI, C.; ZEZELL, D.M.; DE MICHELI, G.
Bacterial reduction in class II furcation after root debridment with or without Nd:YAG laser irradiation.
Brazilian Dental Journal,
v. 15,
p. 90-91,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32740. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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-
KOGLER, V.L.; MAIO, M.; LAGE-MARQUES, J.L.; ZEZELL, D.M.
.
Carbon dioxide laser or cold scalpel on the removal of gingival melanin pigmentation: comparative study.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 90-90,
2004.
Abstract:
Melanin pigmentation is the result of melanin granules produced
by melanocytes present in the basal layer of the oral epithelium.
Gingival physiological melanin pigmentation is symmetric and
persistent, may cause esthetic problems especially in individuals
with a gummy smile. Various techniques have been described for
the removal of melanin pigmentation from the gingival epithelium
and partial thin connective tissue, as chemical agents,
cryosurgery, surgery and gingival grafts. Recently, lasers systems
have been used to coagulate and vaporize cells, promoting controlled
gingival ablation. This study compares clinical efficiency to removal gingival melanin pigmentation in 20 patients with
dioxide carbon laser, and 20 patients with cold scalpel during 30
days after surgery. A dioxide carbon laser (output = 5W; superpulse
= 0,5s; spot size = 2,5mm defocused; focal distance =
5,5cm, Intensity = 102 W/cm2) was irradiated on gingival mucosal
surface. Both techniques presented epithelialization in 15 days.
Both systems are considered effective for removal melanin pigments.
Patient's evaluation with postoperative pain found the carbon
dioxide laser technique superior to the cold scalpel one. After
30 days, the repigmentation occured in 45% of the dioxide carbon
laser patients, and 80% of the cold scalpel patients.
Palavras-Chave:
carbon dioxide lasers;
melanin;
pigments;
lasers
KOGLER, V.L.; MAIO, M.; LAGE-MARQUES, J.L.; ZEZELL, D.M.
Carbon dioxide laser or cold scalpel on the removal of gingival melanin pigmentation: comparative study.
Brazilian Dental Journal,
v. 15,
p. 90-90,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32739. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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-
FARHART, P.B.A.; TANJI, E.Y.; FARHAT, R.P.; ZEZELL, D.M.
; MIYAKAWA, W.; NOGUEIRA, G.E.C..
Model of thermal and optical effects in dental pulp during the neodymium and diode lasers irradiation.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 90-90,
2004.
Abstract:
Applications of high intensity lasers in the enamel and dentine
can produce adverse thermal effects into the pulp. Since the evaluation
thermal effects into the intact pulp is not a solved problem,
extracted teeth models have been used frequently. Current models,
however, simulate only tooth thermal properties, not taking
the remaining radiation in the pulp chamber into account. The
aim of this study was to verify if the remaining radiation from
neodymium and diode lasers that reach the pulp chamber, at the
models using extracted bovine teeth, can causes local thermal
effects. For this purpose, two models were developed using
extracted bovine teeth with their pulp chambers filled with: water
(model 1) and with an optical absorbent (model 2). Models were
radiated with 1 W. The obtained results show that, for both lasers,
the temperature rise in model 2 pulp chamber is: i) up to 11%
higher than in the model 1 when the enamel is radiated and ii) up
to 37% higher than in the model 1 when dentine is radiated (1
mm from the pulp). Thus, remaining radiation into the pulp is relevant
for the above mentioned lasers and doses.
Palavras-Chave:
biological radiation effects;
dentin;
dentistry;
enamels;
laser radiation;
temperature dependence
FARHART, P.B.A.; TANJI, E.Y.; FARHAT, R.P.; ZEZELL, D.M.; MIYAKAWA, W.; NOGUEIRA, G.E.C.
Model of thermal and optical effects in dental pulp during the neodymium and diode lasers irradiation.
Brazilian Dental Journal,
v. 15,
p. 90-90,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32738. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
Como referenciar este item
-
THEODORO, L.H.; GARCIA, V.G.; ZEZELL, D.M.
; HAYPEK, P.; BACHMANN, L.
; SAMPAIO, J.E.C..
Effect of Er:YAG and diode lasers in the adhesion of blood components and in the morphology of irradiated root surfaces.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 89-89,
2004.
Abstract:
The aim of this study was to evaluate in vitro the adhesion of
blood components on root surfaces irradiated with Er:YAG
(2.94??m) and GaAlAs Diode (808 nm) lasers and these effects
on irradiated root surfaces. It was obtained 100 samples of
human teeth. They were scaled and divided into five groups of 20
samples each: G1 (Control); G2 -Er:YAG laser (7.6 J/cm2); G3 - Er:YAG laser (12.9 J/cm2); G4 -Diode laser (90 J/cm2) and G5 -
Diode laser (108 J/cm2). After these treatments were conducted,
10 samples of each group received a blood tissue, and the reminiscent
10 samples did not receive such treatment. After laboratorial
treatments the samples were analysed by scanning electron
microscopy. The results have shown that there were no significant
differences between the Control Group and the groups treated
with Er:YAG laser (p=0,9633 and 0,6229); G4 and G5 were
less effective than the Control Group and the Er:YAG laser
groups (p<0,01). No proposed treatment increased the adhesion
of blood components in a significant way when compared to the
Control Group; although the Er:YAG laser did not interfere in the
adhesion of blood components it caused
more changes on the root surface, while the Diode laser inhibited
the adhesion.
Palavras-Chave:
teeth;
blood;
fibrin;
lasers;
erbium;
in vitro;
morphological changes
THEODORO, L.H.; GARCIA, V.G.; ZEZELL, D.M.; HAYPEK, P.; BACHMANN, L.; SAMPAIO, J.E.C.
Effect of Er:YAG and diode lasers in the adhesion of blood components and in the morphology of irradiated root surfaces.
Brazilian Dental Journal,
v. 15,
p. 89-89,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32737. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
Como referenciar este item
-
EDUARDO, L.R.P.; RIBEIRO, M.S.
; DUARTE, M.; ZEZELL, D.M.
.
Low level laser therapy in treatment of TMJ and masticator muscles disease: biometrical and pain evaluation.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 87-87,
2004.
Abstract:
A sample of 11 patients showing temporomandibular joint disease,
in one or both sides, was selected. Clinical examinations
were performed on patients to define which side was the most
compromised by the disease. Only the worst side was chosen to
be treated by the laser therapy. The Laser Unit used was a diode
laser (AlGaAs) in which the wavelength was 810 nm (infra-red
spectrum). Irradiations were done in tree points of TMJ, with
22,5 J/cm2; two points in the masseter muscle, with 15 J/cm2;
and tree points in the temporal muscle, with 7,5 J/cm2. All points
were irradiated for 30 seconds. The other side, of lesser complaint
received a sham therapy. Treatment was done during two
weeks, tree irradiation with 48 hours interval in the first week,
and two irradiations with the same interval in the second week.
Patients reported improvement in relation to the level of pain,
mainly after the forth irradiation, and the level of pain decreased
until the last day of treatment. In relation to the degree of mouth
opening, the majority of patients showed a significant increase,
mainly after the fifth day of irradiation.
Palavras-Chave:
laser radiation;
therapy;
muscles;
jaw;
pain
EDUARDO, L.R.P.; RIBEIRO, M.S.; DUARTE, M.; ZEZELL, D.M.
Low level laser therapy in treatment of TMJ and masticator muscles disease: biometrical and pain evaluation.
Brazilian Dental Journal,
v. 15,
p. 87-87,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32736. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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-
FREITAS, A.Z.
; ZEZELL, D.M.
; RIBEIRO, A.C.; GOMES, A.S.L.; VIEIRA, N.D.
.
Imagin carious human dental tissue with three-dimensional optical coherence tomography.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 79-79,
2004.
Abstract:
Optical Coherence Tomography (OCT) used in this study, is a
new non invasive optical detection technique. The OCT system
is based on a Michelson interferometer, that generates a crosssectional
image of the teeth with resolution up to 2 microns. The
buccal surface from the third molar teeth was used to induce
caries like lesions. This surface was coated with an acid resistant
nail varnish except a small window. The pH demineralizationremineralization
cycling model was used to produce the lesions.
This cycle was repeated for 9 days and remained in the remineralizing
solution for 2 days. The OCT system was implemented by
using an ultrashort pulse laser (Ti:Al2O3@830nm) with 50fs of
pulse width and average power of 80mW. The laser beam was
focused into the teeth providing a lateral resolution of 10
microns. Image was produced with a lateral and axial scans steps
of 10 microns. After analyzing the surface by OCT it was possible
to produce a tomogram of dentine-enamel junction and it was
compared with the histological image. This OCT system accurately
depicts dental tissue and it was able to detect early caries
in its structure, providing a powerful contactless high resolution
3D images of lesions.
Palavras-Chave:
teeth;
bone tissues;
microstructure;
caries;
three-dimensional calculations;
interferometry;
optical properties;
tomography
FREITAS, A.Z.; ZEZELL, D.M.; RIBEIRO, A.C.; GOMES, A.S.L.; VIEIRA, N.D.
Imagin carious human dental tissue with three-dimensional optical coherence tomography.
Brazilian Dental Journal,
v. 15,
p. 79-79,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32735. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
Como referenciar este item
-
BACHMANN, L.
; DIEBOLDER, R.; HIBST, R.; ZEZELL, D.M.
.
Changes in chemical composition and collagen structure of dentin tissue after erbium laser irradiation.
Brazilian Dental Journal,
v. 15,
Special issue,
p. 78-78,
2004.
Abstract:
The erbium laser light has a great affinity to the water molecule,
which is present in great quantity in biological hard tissues. The
objective of this work is to identify chemical changes by infrared
spectroscopy of irradiated dentin by an Er:YAG - 2.94μm laser.
The irradiation was performed with fluences between 0.365
J/cm2 and 1.94 J/cm2. For the infrared analysis a Fourier transform
infrared spectrometer was used. After the irradiation were
observed: loss of water, alteration of the structure and composition
of the collagen and increase of the OH- radical. These alterations
can be identified by a decrease of the water and OH- band between 3800-2800 cm-1, bands ascribed to collagen structure
between 1400-1100 cm-1. The results show that the erbium laser
changes the structure and composition of the organic matrix, OHradical
and the water composition in the irradiated dentin.
Palavras-Chave:
dentin;
bone tissues;
teeth;
erbium;
lasers
BACHMANN, L.; DIEBOLDER, R.; HIBST, R.; ZEZELL, D.M.
Changes in chemical composition and collagen structure of dentin tissue after erbium laser irradiation.
Brazilian Dental Journal,
v. 15,
p. 78-78,
2004.
Special issue.
Disponível em: http://repositorio.ipen.br/handle/123456789/32734. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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-
ETCHEBEHERE, E.; LIMA, M.; PEREIRA, L.; PAGNANO, R.; BORTOLETTI, E.
; MENGATTI, J.
; BRUNETTO, S.Q.; TAKAHASHI, M.; BRUNETTO, E.; OZELO, M.; SANTOS, A..
Knee radiosynovectomy with Sm-153 hydroxyapatite compared to Y-90 hydroxyapatite: initial results of a prospective trial.
European Journal of Nuclear Medicine and Molecular Imaging,
v. 47,
Supplement 1,
p. S628-S628,
2020.
DOI:
10.1007/s00259-020-04988-4
Abstract:
Aim/Introduction: Introduction: The most common
clinical presentation in hemophilia patients consists of
hemarthrosis. Various treatment strategies aim to control
hemarthrosis to prevent secondary arthropathy, among
them, radiosynovectomy with Y-90 hydroxyapatite (90YHA).
A few studies have shown a lower efficiency of knee
radiosynovectomy with Sm-153 hydroxyapatite (153Sm-
HA) compared to 90Y-HA. Purpose: The purpose of this
investigation was to assess the efficacy and safety of knee
radiosynovectomy with 153Sm-HA compared to 90Y-HA.
Materials and Methods: Forty patients were prospectively
assigned to undergo knee radiosynovectomy with 153Sm-
HA (19 patients) or with 90Y-HA (21 patients). The frequency
of hemarthrosis episodes before and after treatment was
compared. Results: The response to knee radiosynovectomy
stratifying according to radiotracer showed that after 6
months the median response rate with 153Sm-HA was not
significantly different from 90Y-HA (87.5% vs 80.9%; p = 0.576).
However, after 12 months the median response rate of knee
radiosynovectomy with 153Sm-HA was significantly better
than with 90Y-HA (87.5% vs 50%; p = 0.037), respectively. The
reduction of joint bleeding by at least 50%, after 12 months,
was greater in the group of patients treated with 153Sm-HA
compared to 90Y-HA (74% vs 52%), respectively. Conclusion:
Knee radiosynovectomy with high doses of 153Sm-HA is
safe, with an efficiency rate similar that is described in the
literature by the 90Y-HA.
ETCHEBEHERE, E.; LIMA, M.; PEREIRA, L.; PAGNANO, R.; BORTOLETTI, E.; MENGATTI, J.; BRUNETTO, S.Q.; TAKAHASHI, M.; BRUNETTO, E.; OZELO, M.; SANTOS, A.
Knee radiosynovectomy with Sm-153 hydroxyapatite compared to Y-90 hydroxyapatite: initial results of a prospective trial.
European Journal of Nuclear Medicine and Molecular Imaging,
v. 47,
p. S628-S628,
2020.
Supplement 1,
DOI:
10.1007/s00259-020-04988-4.
Disponível em: http://repositorio.ipen.br/handle/123456789/32698. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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-
RAMOS, C.; SOUZA, S.; TOBAR, N.; CASTRO, V.; FRASSON, F.; AMORIM, B.; ETCHEBEHERE, E.; LIMA, M.; MENGATTI, J.
; ARAUJO, E.
; PERINI, E.
; SOUZA, C.; LORAND-METZE, I.; SANTOS, A.; DELAMAIN, M..
Comparison of 68Ga-PSMA and 18F-FDG PET/CT uptake in different lymphoma subtypes: preliminary results.
European Journal of Nuclear Medicine and Molecular Imaging,
v. 47,
Supplement 1,
p. S41-S42,
2020.
DOI:
10.1007/s00259-020-04988-4
Abstract:
Aim/Introduction: Few reports have documented the uptake
of radiolabeled Prostate-Specific Membrane Antigen (PSMA)
in lymphomas [1,2]. It is not known how PSMA uptake
varies among various histological subtypes and how it
correlates with 18F-FDG uptake in lymphomas. This study
aimed to compare 68Ga-PSMA and 18F-FDG in different
lymphoma subtypes. Materials and Methods: Nine randomly
selected patients with biopsy-proven lymphoma -median
age 43 (32-70) years, 5 female - were submitted to whole-body
18F-FDG and 68Ga- PSMA PET/CT (time interval: 1-6
days between procedures). Lymphoma subtypes included:
nodular-sclerosis Hodgkin’s lymphoma (HL; 2 patients);
diffuse large B-cell lymphoma (DLBCL; 1); marginal-zone
lymphoma (2); MALT lymphoma (ML; 1); follicular lymphoma
(FL; 1); lymphoplasmacytic lymphoma (1); and B-cell non-
Hodgkin’s lymphoma, unspecified (BCNHL-U; 1). Eight
patients were under initial staging, and 1 (HL) with disease
relapse after treatment. Two experienced nuclear physicians
analyzed the images by consensus. The intensity of tracer
uptake was visually classified as marked, moderate or mild.
The affected sites (lymph node chains, spleen, diffuse bone
marrow involvement and non-lymphatic focal lesions)
were counted in both sets of images and their respective
maximum SUV (SUVmax) were measured. Results: PSMA PET/
CT was positive in all patients except for one with ML. FDG
PET/CT was positive in all patients. At visual analyses, FDG
uptake was higher than PSMA uptake in all patients, except
for one patient with BCNHL-U (both tracers with similar low-intensity
uptake). The intensity of FDG and PSMA uptake
was respectively classified as marked in 3/9 and 0/8 patients,
moderate in 4/9 and 1/8 and mild in 2/9 and 7/8. One patient
(FL) presented a “mismatch” uptake pattern with different parts of an extensive lesion presenting predominant uptake
of PSMA or FDG. Brain infiltration in one patient (DLBCL) was
more easily identified on PSMA than on FDG images. FDG
detected a total of 58/58 and PSMA 43/58 affected sites in all
patients with a median SUVmax of respectively 5.4 (2.0-31.1)
and 2.8 (1.3-5.4), p<0.0001. The median SUVs of the 43 lesions
with uptake of both tracers was respectively 5.5 (2.0-28.9)
and 2.8 (1.3-P5.4) for FDG and PSMA, p<0.0001. Conclusion:
Distinct lymphoma subtypes present PSMA uptake, with less
intensity than FDG uptake. Although PSMA uptake is usually
mild, several lymphoma subtypes might cause false-positive
results in PSMA PET/CT performed to assess prostate cancer.
RAMOS, C.; SOUZA, S.; TOBAR, N.; CASTRO, V.; FRASSON, F.; AMORIM, B.; ETCHEBEHERE, E.; LIMA, M.; MENGATTI, J.; ARAUJO, E.; PERINI, E.; SOUZA, C.; LORAND-METZE, I.; SANTOS, A.; DELAMAIN, M.
Comparison of 68Ga-PSMA and 18F-FDG PET/CT uptake in different lymphoma subtypes: preliminary results.
European Journal of Nuclear Medicine and Molecular Imaging,
v. 47,
p. S41-S42,
2020.
Supplement 1,
DOI:
10.1007/s00259-020-04988-4.
Disponível em: http://repositorio.ipen.br/handle/123456789/32697. Acesso em: $DATA.
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SOUZA, S.; DELAMAIN, M.; TOBAR, N.; CASTRO, V.; FRASSON, F.; AMORIM, B.; ETCHEBEHERE, E.; MARIANA, K.; MENGATTI, J.
; ARAUJO, E.B.
; PERINI, E.
; SOUZA, C. de; SANTOS, A.; LORANT-METZE, I.; RAMOS, C..
Comparison of 68ga-psma and 18f-fdg pet/ct uptake in different lymphoma.
Hematology, Transfusion and Cell Therapy,
v. 42,
Supplement 1,
p. S48-S48,
2020.
DOI:
10.1016/j.htct.2020.09.086
Abstract:
Objective: Few reports have documented the uptake of radiolabeled Prostate-Specific Membrane Antigen (PSMA) in lymphomas.1, [2] It is not known how PSMA uptake varies among various histological subtypes and how it correlates with 18F-FDG uptake in lymphomas. This study aimed to compare 68Ga-PSMA and 18F-FDG in different lymphoma subtypes.
Methodology: Nine randomly selected patients with biopsy-proven lymphoma with a median age 43 (32–70) years, 5 female – were submitted to whole-body 18F-FDG and 68Ga – PSMA PET/CT (time interval: 1–6 days between procedures). Lymphoma subtypes included: nodular-sclerosis Hodgkin's lymphoma (HL; 2 patients); diffuse large B-cell lymphoma (DLBCL; 1); marginal-zone lymphoma (2); MALT lymphoma (ML; 1); follicular lymphoma (FL; 1); lymphoplasmacytic lymphoma (1); and B-cell non-Hodgkin's lymphoma, unspecified (BCNHL-U; 1). Eight patients were under initial staging and 1 (HL) with disease relapse after treatment. Two experienced nuclear physicians analyzed the images by consensus. The intensity of tracer uptake was visually classified as marked, moderate or mild. The affected sites (lymph node chains, spleen, diffuse bone marrow involvement and non-lymphatic focal lesions) were counted in both sets of images and their respective maximum SUV (SUVmax) were measured.
Results: PSMA PET/CT was positive in all patients except for one with ML. FDG PET/CT was positive in all patients. At visual analyses, FDG uptake was higher than PSMA uptake in all patients, except for one patient with BCNHL-U (both tracers with similar low-intensity uptake). The intensity of FDG and PSMA uptake was respectively classified as marked in 3/9 and 0/8 patients, moderate in 4/9 and 1/8 and mild in 2/9 and 7/8. One patient (FL) presented a “mismatch” uptake pattern with different parts of an extensive lesion presenting predominant uptake of PSMA or FDG. Brain infiltration in one patient (DLBCL) was more easily identified on PSMA than on FDG images. FDG detected a total of 58/58 and PSMA 43/58 affected sites in all patients with a median SUVmax of respectively 5.4 (2.0–31.1) and 2.8 (1.3–5.4), p < 0.0001. The median SUVs of the 43 lesions with uptake of both tracers was respectively 5.5 (2.0–28.9) and 2.8 (1.3–5.4) for FDG and PSMA, p < 0.0001.
Conclusion: Distinct lymphoma subtypes present PSMA uptake, with less intensity than FDG uptake. Although PSMA uptake is usually mild, several lymphoma subtypes might cause false-positive results in PSMA PET/CT performed to assess prostate cancer.
SOUZA, S.; DELAMAIN, M.; TOBAR, N.; CASTRO, V.; FRASSON, F.; AMORIM, B.; ETCHEBEHERE, E.; MARIANA, K.; MENGATTI, J.; ARAUJO, E.B.; PERINI, E.; SOUZA, C. de; SANTOS, A.; LORANT-METZE, I.; RAMOS, C.
Comparison of 68ga-psma and 18f-fdg pet/ct uptake in different lymphoma.
Hematology, Transfusion and Cell Therapy,
v. 42,
p. S48-S48,
2020.
Supplement 1,
DOI:
10.1016/j.htct.2020.09.086.
Disponível em: http://repositorio.ipen.br/handle/123456789/32696. Acesso em: $DATA.
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ANGELOCCI, L.
; NOGUEIRA, B.R.
; SOUZA, C.D. de
; ZEITUNI, C.A.
; ROSTELATO, M.E.C.M.
.
Assessing Ir-192 as an alternative to I-125 in ophthalmic treatment.
Radiotherapy and Oncology,
v. 152,
Supplement 1,
p. S735-S736,
2020.
DOI:
10.1016/S0167-8140(21)01402-X
Abstract:
Purpose or Objective: Brachytherapy sources for ocular melanoma usually
contain Co-60, I-125, Pd-103 or Ru/Rh-106 as
radionuclides. Ir-192 is not a preconized radioactive
material for this purpose, although it is used for other
brachytherapy applications. Higher mean energy from
Ir-192 emission (ca. 380 keV) may be a reason for the
preference of I-125 (35 keV) or Pd-103 (21 keV) over it,
since low penetration is desired on the small structures of
the human eye. This is not, however, an excluding
criterion, considering Co-60 and Ru/Rh-106 have even
higher mean energies.
The demand in Brazil for lower-cost seeds to treat ocular
melanoma lead to the development of an Ir-192 seed to
make treatment more accessible, but since it is not used
as an ophthalmic brachytherapy source, before its
dosimetry is considered, one should care about the
possibility of using it over more stablished materials.
Considering this, the aim of this work is to assess the
possibility of using Ir-192 seeds as ophthalmic
brachytherapy sources by comparing some dosimetric
parameters of a new seed model with the most stablished
I-125 seed in literature, OncoSeed 6711. Material and Methods: As an initial study on the topic, this work relies only on
Monte-Carlo simulations using MCNP4C transport code.
Parameters analyzed are air-kerma strength, dose-rate
constant and depth-dose curve, attention given to points
within the human eye dimensions. The medium considered
was homogeneous water, as it is a good approximation to
the eye tissues in terms of composition and density and
allows for future comparisons with TG-43 based
calculations.
OncoSeed 6711 is not produced anymore, but its long term
as the reference source for dosimetry was considered. A
20 mm COMS ophthalmic applicator was also modeled and
considered to be fully loaded with each seed model to
compare the same parameters at a realistically clinical
approach. Results: As expected, due to the higher energy of the Ir-192
emission spectrum, dose fall-off on the transversal axis of
the seeds is less pronounced for the new seed model. The
steeper dose gradient for I-125 is also visible on the doserate
constant value. The effect of using a COMS applicator
only strengthens this characteristic. Depth-dose curves
were calculated up to the distance of 5 cm, both for a
single seed and for an applicator fully loaded with 24
seeds. All the eye components relevant for dosimetry are located within this range, like the cells of the crystallin
and the optical nerve. Conclusion: If one expects to use Ir-192 as an alternative to I-125 in
ophthalmic cancer treatment, at least the dosimetry
following TG-43 protocol should be carried with utmost
attention, as undesirable dose to healthy nearby tissues is
unavoidable. Crafting a different applicator most suited
for this radionuclide is a possibility that can be taken into
account. Another recommendation is to go beyond TG-43
water-based protocol and actually estimate dose to
relevant eye components.
ANGELOCCI, L.; NOGUEIRA, B.R.; SOUZA, C.D. de; ZEITUNI, C.A.; ROSTELATO, M.E.C.M.
Assessing Ir-192 as an alternative to I-125 in ophthalmic treatment.
Radiotherapy and Oncology,
v. 152,
p. S735-S736,
2020.
Supplement 1,
DOI:
10.1016/S0167-8140(21)01402-X.
Disponível em: http://repositorio.ipen.br/handle/123456789/32695. Acesso em: $DATA.
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BELLEZZO, M.
; FONSECA, G.P.; VONCKEN, R.; VERRIJSSEN, A.; VAN BEVEREN, C.; ROELOFS, E.; YORIYAZ, H.
; RENIERS, B.; VAN LIMBERGEN, E.J.; BARBEE, M.; VERHAEGEN, F..
MAASTRO applicator, a novel rectal applicator for contact brachytherapy with 192Ir HDR sources.
Radiotherapy and Oncology,
v. 152,
Supplement 1,
p. S1102-S1103,
2020.
DOI:
10.1016/S0167-8140(21)01990-3
Abstract:
Purpose or Objective: The standard care for rectal cancer includes surgery,
which may be avoided if complete response is achieved,
e.g. with chemoradiotherapy (EBCRT) or external beam
radiotherapy EBRT, adopting a watch and wait strategy.
Studies report a local regrowth reduction from 30% (EBCRT
alone) to 11% when EBCRT is associated with a radiation
boost using 50 kV x-rays (CXB), technique that allows a
high dose delivery to a highly selective volume, allowing
preservation of organs at risk (OAR) and low toxicity.
However, CXB is not widely adopted due to its low costeffectiveness.
Hence, the MAASTRO applicator was
developed to deliver a dose distribution similar to those
generated by CXB devices, but using HDR 192Ir sources, as
a cost-effective alternative to CXB, with possibility of
integration to treatment planning systems (TPS). Material and Methods: Fig 1-a shows the applicator design, a cylindrical
applicator with 5 channels and a slanted edge, using its tip. Results: Due to the applicator geometry, the most distal position
of the source in each channel doesn’t reach the sharp edge
of the applicator, resulting in an effective treatment
surface (high dose region shown in Fig 1 a and b) of
approximately 20 x 20 mm2, which is smaller than the
contact surface. The resulting dose falloff is steeper than
the one resulting from the P50 with a 22 mm applicator.
With the dose falloff normalized at 2mm, the relative dose
values delivered at depths of 0, 2, 5 and 10 mm are,
respectively, 130, 100, 70 and 43% for the P50 and 140,
100, 67 and 38% for the applicator. The time required to
deliver an average dose of 32 Gy to the treatment surface
of the applicator is 5m30s for a 40700 U source (new
source) and 8m30s for a 20350 U source (source to be
replaced), including the time required to
perform obstruction verification before irradiation. The
applicator delivers a high dose to a small target volume
while the lateral shielding spares normal tissues in all
directions other than the contact surface. Conclusion: The MAASTRO applicator was designed to deliver dose
distributions similar to those of CXB devices using 192Ir
HDR sources. The applicator has the advantage of TPS
integration, increasing the degrees of freedom to
modulate the dose distribution.
BELLEZZO, M.; FONSECA, G.P.; VONCKEN, R.; VERRIJSSEN, A.; VAN BEVEREN, C.; ROELOFS, E.; YORIYAZ, H.; RENIERS, B.; VAN LIMBERGEN, E.J.; BARBEE, M.; VERHAEGEN, F.
MAASTRO applicator, a novel rectal applicator for contact brachytherapy with 192Ir HDR sources.
Radiotherapy and Oncology,
v. 152,
p. S1102-S1103,
2020.
Supplement 1,
DOI:
10.1016/S0167-8140(21)01990-3.
Disponível em: http://repositorio.ipen.br/handle/123456789/32694. Acesso em: $DATA.
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ZANINI, NATHALIA
; ZAMATARO, CLAUDIA B.
; RABELO, THAIS F.
; JUVINO, AMANDA C.
; KUCHAR, NIELSEN G.
; CASTRO, PEDRO
; ANA, PATRICIA da; ZEZELL, DENISE
.
Analysis of ceramic laminates removal with Er,Cr:YSGG laser by optical coherence tomography.
Photobiomodulation, Photomedicine, and Laser Surgery,
v. 37,
n. 10,
p. A22-A22,
2019.
DOI:
10.1089/photob.2019.29013.abstracts
Abstract:
Porcelain laminated veneers have been widely used. For wear of
hard tissue such as enamel and dentin, the diamond rotary instrument
is the most traditional, but the laser has become recently used
to remove aesthetic facets. Optical coherence tomography (OCT)
used as an optical biopsy, is important for morphological analysis
and attenuation coefficient is related to the property of the photons to
be scattered by the samples. After approval by the Ethics Committee,
the present study investigated the detachment of 30 ceramic
E-max fragments cemented in human dental enamel of dimensions
3mm x 3mm x 0.7mm with 3 types of resin cements, RelxY Veneer,
Relx U200 and Variolink Veneer. The samples (Enamel +
Ceramic Fragment) were randomly distributed in the 3 groups and
cemented according to the manufacturer. After that, they were
prepared for irradiation with the Er,Cr: YSSG laser under predetermined
conditions (3.5 and 3W, 20Hz, 60% water and 40% air
flow). OCT analysis was done before and after irradiation. We observed
that themorphological changes of the enamel surface showed an
increased surface area due to the cement remaining in the enamel.We
concluded that the Er, Cr: YSGG laser, when used in the irradiation
protocol tested, seems to be a safe tool for the removal of laminates.
ZANINI, NATHALIA; ZAMATARO, CLAUDIA B.; RABELO, THAIS F.; JUVINO, AMANDA C.; KUCHAR, NIELSEN G.; CASTRO, PEDRO; ANA, PATRICIA da; ZEZELL, DENISE.
Analysis of ceramic laminates removal with Er,Cr:YSGG laser by optical coherence tomography.
Photobiomodulation, Photomedicine, and Laser Surgery,
v. 37,
n. 10,
p. A22-A22,
2019.
DOI:
10.1089/photob.2019.29013.abstracts.
Disponível em: http://repositorio.ipen.br/handle/123456789/32224. Acesso em: $DATA.
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-
RABELO, THAIS F.
; ZAMATARO, CLAUDIA B.
; ZANINI, NATHALIA
; JUVINO, AMANDA C.
; KUCHAR, NIELSEN G.
; CASTRO, PEDRO A.A.
; ZEZELL, DENISE M.
.
Removal of laminates with Er,Cr:YSGG laser from dental enamel submitted to gamma radiation.
Photobiomodulation, Photomedicine, and Laser Surgery,
v. 37,
n. 10,
p. A21-A21,
2019.
DOI:
10.1089/photob.2019.29013.abstracts
Abstract:
Background: Modern Dentistry is characterized by the search for
aesthetic perfection in the smile. Many efforts are made regarding
procedures related to manufacturing, cementing, maintenance and
removal of ceramic laminates. The laser removal of laminates has
become more frequent when the aesthetical procedures needs to
be replaced. On the other hand, the head and neck cancer treatment
causes comorbidities in the buccal environment when laminates
are in place.
Objective: To evaluate in vitro the removal of ceramic fragments
by means of Er,Cr:YSGG laser irradiation, after gamma
radiation.
Methods: 20 Lithium disilicate veneers were cemented with
Variolink to human dental enamel and then samples were irradiated
with 0.07 kGy. After gamma irradiation, the laminates removal
with Er,Cr:YSGG laser was performed. The control group
was not subjected to gamma radiation. Dental enamel samples were
analyzed by Scanning Electron Microscopy (SEM), Fourier
Transformed Infrared Spectroscopy (FTIR) and Surface Microhardness
Loss (SMH). Results: SEM has shown that less cement
was found after removal of the facets in gamma irradiated group
when compared to the control. For both groups there was alteration
of the SMH possibly due to the use of the adhesive system. When
performed intra group analysis, the sample being its own control of
baseline in the FTIR analysis, there were no band shifts or formation
of new compounds on the surface of human dental enamel.
Conclusions: The Er,Cr:YSGG laser is an effective alternative
for laminates removal in human dental enamel even when it is
subjected to gamma radiation.
RABELO, THAIS F.; ZAMATARO, CLAUDIA B.; ZANINI, NATHALIA; JUVINO, AMANDA C.; KUCHAR, NIELSEN G.; CASTRO, PEDRO A.A.; ZEZELL, DENISE M.
Removal of laminates with Er,Cr:YSGG laser from dental enamel submitted to gamma radiation.
Photobiomodulation, Photomedicine, and Laser Surgery,
v. 37,
n. 10,
p. A21-A21,
2019.
DOI:
10.1089/photob.2019.29013.abstracts.
Disponível em: http://repositorio.ipen.br/handle/123456789/32223. Acesso em: $DATA.
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KUCHAR, NIELSEN G.
; ZAMATARO, CLAUDIA B.
; RABELO, THAIS
; JUVINO, AMANDA
; ZANINI, NATHALIA
; CASTRO, PEDRO
; ZEZELL, DENISE M.
.
Calcium analysis of gamma sterilized human dentin submmited to Er,Cr:YSGG laser irradiation.
Photobiomodulation, Photomedicine, and Laser Surgery,
v. 37,
n. 10,
p. A18-A19,
2019.
DOI:
10.1089/photob.2019.29013.abstracts
Abstract:
Studies report that gamma radiation changes the oral cavity of patients
submitted to radiotherapy. These changes include xerostomia,
resulting in an unsaturation of calciumand phosphate in the oral cavity.
However, human enamel samples isolated from the altered oral environmentwhen
irradiatedwithgamma radiation at doses of up to 25kGy
(much higher than radiotherapy doses) were shown to have undergone
no changes in molecular hardness or micro surface. After approval of
the Research Ethics Committee, 20 human dentin samples were
evaluated before and after irradiation, split randomly into control group
(n = 10) and irradiated group (n = 10) treated with 25.0 kGy at the Co60
multipurpose irradiator and irradiated with Er,Cr:YSGG laser in the
parameters: 8.5 J/cm2 in the Fourier Transform Infrared Spectroscopy
(FTIR), % surface Microhardness loss and Scanning Electron Microscopy
(SEM). At the end, acidic biopsies were performed to quantify
the concentration of calcium present in the samples. In the results of
FTIR analysis differences were found only in the bands of organic
content and in the inorganic content, difference were not found between
before and after irradiations. The EDS and % Surface Microhardness
loss analysis corroborates these findings, as well as no
significant loss of the Calcium content before and after their radiation
with Co60 at 25 kGy and with the Er,Cr:YSGG laser. These findings
lead us to a new hypothesis of behavior of the hydroxyapatite crystal
submitted to the gamma irradiation.
Palavras-Chave:
teeth;
radiotherapy;
gamma radiation;
radiation doses;
calcium
KUCHAR, NIELSEN G.; ZAMATARO, CLAUDIA B.; RABELO, THAIS; JUVINO, AMANDA; ZANINI, NATHALIA; CASTRO, PEDRO; ZEZELL, DENISE M.
Calcium analysis of gamma sterilized human dentin submmited to Er,Cr:YSGG laser irradiation.
Photobiomodulation, Photomedicine, and Laser Surgery,
v. 37,
n. 10,
p. A18-A19,
2019.
DOI:
10.1089/photob.2019.29013.abstracts.
Disponível em: http://repositorio.ipen.br/handle/123456789/32222. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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-
ZAMATARO, CLAUDIA B.
; RABELO, THAIS F.
; ZANINI, NATHALIA
; JUVINO, AMANDA C.
; KUCHAR, NIELSEN G.
; CASTRO, PEDRO A.L.
; ANA, PATRICIA A. da; ZEZELL, DENISE M.
.
Er,Cr:YSGG laser irradiation associated to fluoride for in situ model using gamma sterilized dentin and enamel.
Photobiomodulation, Photomedicine, and Laser Surgery,
v. 37,
n. 10,
p. A13-A13,
2019.
DOI:
10.1089/photob.2019.29013.abstracts
Abstract:
The in situ intraoral model uses human dental enamel samples
(HDE) in order to analyse the de-remineralization processes using
the buccal environment without interfering into the patients’
natural dentition. The main ethical concern from this model is the
biosafety. Gamma radiation is a very efficient sterilization method
that is not expected to alter the mineral content of the hard tissues,
avoiding biases in the results. Thus 40 HDE samples were irradiated
through a source of 60Co multipurpose irradiator aiming
complete sterilization (25 KGy/h) with the purpose of accumulating
the native plaque on them at an in situ study. An Er,Cr:YSGG laser
was used alone and in combination with the topical applications of:
1-dentifrice (1,100 lg F-/g) or 2-APF (12,300 lg F-/g). Morphological
analyses were performed by scanning electron microscopy
(SEM), determination of alkali-soluble fluoride concentration by
specific ion electrode and microhardness determination. Then, the
15 volunteers used palatal devices containing previously treated
HDE samples and remained using F dentifrice. The FTIR findings
established that gamma radiation could be used aiming HDE
sterilization. The Knoop hardness number was within the range
of that of natural dentin of human origin. X-ray fluorescence
shows that irradiated dentin has great similarity with natural
dentin from the point of view of chemical composition. SEM
analyses showed that there was no thermal damage or interprismatic
morphological changes in the hydroxyapatite structure of
human dental dentin outside the buccal environment when using
doses of gamma irradiation up to 25 kGy.
Palavras-Chave:
teeth;
enamels;
sterilization;
lasers;
irradiation
ZAMATARO, CLAUDIA B.; RABELO, THAIS F.; ZANINI, NATHALIA; JUVINO, AMANDA C.; KUCHAR, NIELSEN G.; CASTRO, PEDRO A.L.; ANA, PATRICIA A. da; ZEZELL, DENISE M.
Er,Cr:YSGG laser irradiation associated to fluoride for in situ model using gamma sterilized dentin and enamel.
Photobiomodulation, Photomedicine, and Laser Surgery,
v. 37,
n. 10,
p. A13-A13,
2019.
DOI:
10.1089/photob.2019.29013.abstracts.
Disponível em: http://repositorio.ipen.br/handle/123456789/32221. Acesso em: $DATA.
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JUVINO, AMANDA C.
; ZAMATARO, CLAUDIA B.
; RABELO, THAIS F.
; KUCHAR, NIELSEN G.
; ZANINI, NATHALIA
; CASTRO, PEDRO
; ZEZELL, DENISE
.
In vitro determination of the critical pH demineralization of human dental enamel irradiated with Nd:YAG laser associated with fluoridated product.
Photobiomodulation, Photomedicine, and Laser Surgery,
v. 37,
n. 10,
p. A8-A9,
2019.
DOI:
10.1089/photob.2019.29013.abstracts
Abstract:
The use of fluoride products associated with high intensity laser
irradiation are beneficial for dental caries prevention because it
increases the surface area, improving the formation of fluorapatite
(FA), which gives greater acid-resistance of enamel against bacterial
acids. The objective of this study is to determine the critical
pH of dental enamel treated with acid fluoride phosphate
12,300 lF-/g (APF) and Nd:YAG laser 84 J/cm2, as there is no
precedent to determine this pH. The study consisted of 4 groups
(n = 15): G1: Negative Control; G2: APF; G3: Nd:YAG; G4:
APF + Nd:YAG. Each group was randomized into three subgroups
(n = 5) for pH cycling. The cycling was designed to simulate three
conditions: below critical pH of enamel hydroxyapatite (pH 5.0);
pH below critical for hydroxyapatite and fluorapatite (pH 4.5);
condition further below the critical situation to investigate extent
of acid resistance of the enamel (pH 4.0). The samples were analyzed by scanning electron microscopy (SEM), Fourier infrared
spectroscopy (FTIR) and solutions were quantified fluoride
(specific ion electrode) and phosphate (colorimetric method). In
SEM and FTIR (phosphate band) at pH 5, only the APF and APF
+ Nd:YAG groups did not present demineralization. At pH 4.5,
only the APF + Nd:YAG group was integrated. At pH 4, APF +
Nd:YAG showed signs of mild demineralization while the other
groups showed aggressive signals. It is concluded that the irradiated
fluorapatite has critical dissolution pH different from fluorapatite
formed only with the application of fluorine.
Palavras-Chave:
teeth;
enamels;
fluorides;
phosphates;
ph value;
laser radiation
JUVINO, AMANDA C.; ZAMATARO, CLAUDIA B.; RABELO, THAIS F.; KUCHAR, NIELSEN G.; ZANINI, NATHALIA; CASTRO, PEDRO; ZEZELL, DENISE.
In vitro determination of the critical pH demineralization of human dental enamel irradiated with Nd:YAG laser associated with fluoridated product.
Photobiomodulation, Photomedicine, and Laser Surgery,
v. 37,
n. 10,
p. A8-A9,
2019.
DOI:
10.1089/photob.2019.29013.abstracts.
Disponível em: http://repositorio.ipen.br/handle/123456789/32220. Acesso em: $DATA.
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-
CARDOSO, ELISABETH C.L.
; PARRA, DUCLERC F.
; SCAGLIUSI, SANDRA R.
; LUGAO, ADEMAR B.
.
Influence of particle size of bio-calcium carbonate used as reinforcement of PBAT/PLA bio-based foams compatibilized with ionizing radiation.
Journal of Bioremediation & Biodegradation,
v. 10,
p. 42-42,
2019.
Abstract:
Bio-filler from eggshells as reinforcement of bio-based polymers are based on their principle benefits such as good
strength and stiffness besides being an environmental friendly, degradable and renewable material. Eggshell
is an agricultural waste largely considered as garbage and discarded mostly because it contributes to pollution.
Biodegradable polymers as PLA (poli-lactic acid) and PBAT (butylene adipate co-terephthalate) are thermoplastics
which can be processed using most conventional polymer processing methods. PLA is high in strength and modulus
(63 MPa and 3.4 GPa, respectively) but brittle (strain at break 3.8%) while PBAT is flexible and tough (strain at break
~710%). In order to reduce interfacial tension exhibited by PLA/PBAT blend, compatibilization is fundamental:
herein it was used as compatibilizing agent PLA previously e-beam irradiated at 150 kGy: ionizing radiation induces
compatibilization by free radicals, improving the dispersion and adhesion of blend phases, without the use of
chemical additives and at room temperature. PLA/PBAT, 65/35 blend with bio-filler, from avian eggs, of 38 and 75
µm particle size were prior homogenized in a co-rotating twin-screw extruder and further foamed in a mono-screw
extruder, by using CO2 as Physical Blowing Agent (PBA). Characterizations involved: Melt Index, DSC, TGA, FTIR,
SEM, XRD and mechanical essays.
Palavras-Chave:
calcium carbonates;
biodegradation;
eggs;
polymers;
fillers
CARDOSO, ELISABETH C.L.; PARRA, DUCLERC F.; SCAGLIUSI, SANDRA R.; LUGAO, ADEMAR B.
Influence of particle size of bio-calcium carbonate used as reinforcement of PBAT/PLA bio-based foams compatibilized with ionizing radiation.
Journal of Bioremediation & Biodegradation,
v. 10,
p. 42-42,
2019.
Disponível em: http://repositorio.ipen.br/handle/123456789/32203. Acesso em: $DATA.
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PARRA, DUCLERC F.
; SILVA, ORELIO L. da
; KOMATSU, LUIZ G.
; CARDOSO, ELIZABETH C.L.
; LUGAO, ADEMAR B.
.
Nanocomposites foams of polypropilene modified by ionizing radiation containing CaCo3/ag° nanoparticles of bio-calcium carbonate-study of bactericidal effect.
Journal of Bioremediation & Biodegradation,
v. 10,
p. 41-41,
2019.
Abstract:
This paper presents a study of high melting strengh polypropylene (HMSPP) foams by gamma irradiation with
insertion of silver nanoparticles (AgNPs) adsorbed in carrier of CaCO3 (natural source) aiming bactericidal
effect. The use of silver (Ag°) gives important antibacterial property since silver is highly toxic against bacterae. The
HMSPP matrix was processed in a twin screw extruder under CO2 atmosphere and polypropylene nanocomposites
(HMSPP-AgNC) were obtained in different concentrations of silver. The material was characterized by scanning
electron microscopy (SEM), energy dispersive spectroscopy (EDS), field emission scanning electron microscopy
(FESEM), X-ray diffraction spectroscopy (XRD), cytotoxicity assay and reduction colony-forming-unit (CFU).
The analyzed foams showed spherical clusters and homogeneous regions with good distribution of the silver
nanoparticles. Furthermore, the HMSPP@AgNCs foams exhibited a antibacterial efficiency against E. coli and S.
aureus due to the presence of the biocidal silver nanoparticles.
Palavras-Chave:
germicides;
sterilization;
foams;
polypropylene;
gamma radiation;
calcium carbonates
PARRA, DUCLERC F.; SILVA, ORELIO L. da; KOMATSU, LUIZ G.; CARDOSO, ELIZABETH C.L.; LUGAO, ADEMAR B.
Nanocomposites foams of polypropilene modified by ionizing radiation containing CaCo3/ag° nanoparticles of bio-calcium carbonate-study of bactericidal effect.
Journal of Bioremediation & Biodegradation,
v. 10,
p. 41-41,
2019.
Disponível em: http://repositorio.ipen.br/handle/123456789/32198. Acesso em: $DATA.
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PETERSEN, ALAN; TACCHEO, STEFANO; MIROV, SERGEY; NILSSON, JOHAN; PASK, HELEN; SARACENO, CLARA; WETTER, NIKLAUS
; WU, RUIFEN.
Focus issue introduction: Advanced Solid-State Lasers 2020.
Optics Express,
v. 29,
n. 6,
p. 8365-8367,
2021.
DOI:
10.1364/OE.423636
Abstract:
This Joint Issue of Optics Express and Optical Materials Express features 15 articles written by authors who participated in the international online conference Advanced Solid State Lasers held 13–16 October, 2020. This review provides a summary of the conference and these articles from the conference which sample the spectrum of solid state laser theory and experiment, from materials research to sources and from design innovation to applications.
Palavras-Chave:
lasers;
solid state lasers;
radiation sources;
meetings
PETERSEN, ALAN; TACCHEO, STEFANO; MIROV, SERGEY; NILSSON, JOHAN; PASK, HELEN; SARACENO, CLARA; WETTER, NIKLAUS; WU, RUIFEN.
Focus issue introduction: Advanced Solid-State Lasers 2020.
Optics Express,
v. 29,
n. 6,
p. 8365-8367,
2021.
DOI:
10.1364/OE.423636.
Disponível em: http://repositorio.ipen.br/handle/123456789/32049. Acesso em: $DATA.
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PETERSEN, ALAN; TACCHEO, STEFANO; MIROV, SERGEY; NILSSON, JOHAN; PASK, HELEN; SARACENO, CLARA; WETTER, NIKLAUS
; WU, RUIFEN.
Focus issue introduction: Advanced Solid-State Lasers 2020.
Optical Materials Express,
v. 11,
n. 4,
p. 952-954,
2021.
DOI:
10.1364/OME.423641
Abstract:
This Joint Issue of Optics Express and Optical Materials Express features 15 articles written by authors who participated in the international online conference Advanced Solid State Lasers held 13–16 October, 2020. This review provides a summary of the conference and these articles from the conference which sample the spectrum of solid state laser theory and experiment, from materials research to sources and from design innovation to applications.
Palavras-Chave:
lasers;
solid state lasers;
radiation sources;
meetings
PETERSEN, ALAN; TACCHEO, STEFANO; MIROV, SERGEY; NILSSON, JOHAN; PASK, HELEN; SARACENO, CLARA; WETTER, NIKLAUS; WU, RUIFEN.
Focus issue introduction: Advanced Solid-State Lasers 2020.
Optical Materials Express,
v. 11,
n. 4,
p. 952-954,
2021.
DOI:
10.1364/OME.423641.
Disponível em: http://repositorio.ipen.br/handle/123456789/32048. Acesso em: $DATA.
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PARRA, DUCLERC F.
; SANTOS, VINICIUS J. dos
; TAMIÃO, ANGELICA
.
Development of poly (n-vinyl-2-pyrrolidone) hydrogels containing nanosilver and laponite clay for modulation of neomycin.
Journal of Astrophysics & Aerospace Technology,
v. 8,
n. 2,
p. 3-3,
2020.
Abstract:
These Biomaterials widely used in biomedicine as wound
dressings are composed by hydrogels. They offer ideal
conditions for wound treatment, such as maintaining the wound
environment humid, autolytic debridement, accelerated healing,
ability to absorb large amounts of fluids without losing their
physical integrity, drug release in a controlled manner
protecting infected wounds that are constantly exposed to
environment contaminated with microorganisms. These
dressings are formed by one or more polymeric components
which, when cross-linked, form three-dimensional networks.
They have a high degree of flexibility that is very similar to
natural living tissues. The general objective of this work was to
synthesize by radiation hydrogel membranes containing PVP,
PEG, agar, RD laponite in a neomycin and nanosilver release
system that promotes a synergistic action between these two
antimicrobial agents. Membranes with different concentrations
of polymer matrix / clay were produced by irradiation at a dose
of 25 kGy of gamma 60Co source, and characterized by
techniques: swelling analysis, thermogravimetric analysis
(TGA), differential scanning calorimetry (DSC), tensile tests,
parallel plate rheometry. It can be seen that G 'and G "are
approximately parallel within the studied range. G 'is greater
than G' 'and both showed little frequency dependence. This
performance is the expected for the gels, in which the elastic
behavior of the sample predominates over its viscous behavior.
The clay-polymer interactions increases gel cross-linking,
which provides an increase in their mechanical properties. In
parallel, nanoparticles of silver coupled to clay (Laponite) were
synthesized in which their properties were verified by means of
UV-Vis, MET, ICP-OES and microbiological activity. The
study of the microbiological activity of AgNPs-Laponite
nanoparticles on the reduction of microorganisms in culture
indicated that most of the nanoparticles, synthesized from the
reduction of silver ions on the surface of Laponite, have activity against Pseudomonas aeruginosa, Staphylococcus aureus,
Escherichia coli, Candida albicans.
Palavras-Chave:
wounds;
hydrogels;
pvp;
healing;
biological recovery
PARRA, DUCLERC F.; SANTOS, VINICIUS J. dos; TAMIÃO, ANGELICA.
Development of poly (n-vinyl-2-pyrrolidone) hydrogels containing nanosilver and laponite clay for modulation of neomycin.
Journal of Astrophysics & Aerospace Technology,
v. 8,
n. 2,
p. 3-3,
2020.
Disponível em: http://repositorio.ipen.br/handle/123456789/31833. Acesso em: $DATA.
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CARDOSO, ELIZABETH C.L.
; PARRA, DUCLERC F.
; SCAGLIUSI, SANDRA R.
; LUGAO, ADEMAR B.
.
Study of PBAT/PLA bio-based blends reinforced with chicken eggshell nano powder compatibilized with ionizing radiation.
Astrophysics & Aerospace Technology,
v. 8,
n. 2,
p. 11-11,
2020.
Abstract:
Eggshell is a solid waste, with production of several tons per day and it is mostly sent to landfills at a high management cost. A few used of eggshell include: fertilizing the garden, cleaning pots and pans, seed starter, feeding birds, making bone broth, etc… Nevertheless, chicken eggshell biowaste has recently been used to substitute calcium carbonate (CaCO3), due to its reinforcing property and low price. It is economically viable to transform eggshell waste to acquire new values, transforming it into a bioplastic: a biodegradable polymer made from bio-mass. The surprising strength of eggshells endorses their application for reinforcement of biodegradable polymers herein studied: PLA (poly-lactic acid) and PBAT (butylene adipate co-terephthalate). PLA is derived from renewable sourced: polylactic acid plastics are sturdier and capable to be blend with conventional petroleum polymers; nevertheless, they exhibit a narrow process window and low thermal stability, besides an inherent high cost. PBAT, as PLA, is a biodegradable aliphatic polyester, although a synthetic polymer based on fossil resources. By incorporating PBAT in PLA it is expected to improve flexibility of PBAT/PLA blend. Previous studies using PBAT/PLA, 50/50 blends containing 15% of chicken eggshell 125 µm improved mechanical behavior of net blends: values for both force and strain practically doubled, proving the effective reinforcement action of calcium carbonate extracted from eggshells. Conventional polymer processing methods can be used in both PLA and PBAT compositions. Due to common incompatibility between PLA and PBAT, considering their extreme glass transition temperatures: 62 º C and – 30 º C, respectively, it is required a compatibilizer to accomplish or increase their interaction. Herein it was used PLA previously e-beam radiated at 150 kGy, as compatibilizing agent: ionizing radiation induces compatibilization by free radicals, improving the dispersion and adhesion of blend phases, without the use of chemical additives and at room temperature. Herein there were
prepared bio-composite PBAT/PLA 82/18 blends with 2.5, 5.0
and 10.0 % of eggshell nano-powder, 161 nm, in average, and
5.0 % of PLA 150 kGy e-beam radiated were homogeneized in
a co-rotating twin-screw extruder. Subsequent investigations
included: Differential Scanning Calorimeter (DSC), Thermal
Gravimetric Analysis (TGA), Fourier Transmittance Infrared
(FTIR), X-Ray Diffraction (XRD), Tensile Strength and
Elongation at Break
Palavras-Chave:
eggs;
chickens;
fertilizers;
solid wastes;
ionizing radiations;
nanopowders
CARDOSO, ELIZABETH C.L.; PARRA, DUCLERC F.; SCAGLIUSI, SANDRA R.; LUGAO, ADEMAR B.
Study of PBAT/PLA bio-based blends reinforced with chicken eggshell nano powder compatibilized with ionizing radiation.
Astrophysics & Aerospace Technology,
v. 8,
n. 2,
p. 11-11,
2020.
Disponível em: http://repositorio.ipen.br/handle/123456789/31792. Acesso em: $DATA.
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VILLAS BOAS, CRISTIAN A.W.
; MENGATTI, JAIR
; PASSOS, PRISCILA
; VIEIRA, DANIEL
; ARAUJO, ELAINE B. de
.
In vitro response of 177Lu-PSMA-617 with two different specific activities.
Journal of Nuclear Medicine,
v. 61,
supplement 1,
2020.
Abstract:
Introduction: PSMA-617 radiolabeled with lutetium-177 has shown good results in compassionate studies around the world. Being a receptor-specific radiopharmaceutical, the specific activity (SA) of the preparation may represent an important factor for therapeutic efficacy. Lutetium-177 can be produced by two different routes: with ytterbium-176 (Non-carrier-added or NCA) and with lutetium-176 (Carrier-added or CA). The SA (MBq/ug) of the labeled PSMA varies accordingly to each lutetium. For NCA lutetium, the radiolabeling procedure is based on the SA of 74 MBq/ug. When the radiolabeling is performed with CA lutetium, SA is determined by the molar ratio of 2.1:1 (PSMA moles/lutetium moles declared in the certificate), resulting in lower SA than NCA. This work evaluated the influence of specific activity of 177Lu-PSMA-617 on in vitro specific binding assays (saturation, competition and internalization). Materials and Methods: Radiolabeling of PSMA-617 (ABX, Germany) with lutetium-177 was performed in heating block at 90°C for 30 minutes with sodium ascorbate (0.5 M pH 4.7) as buffer. For NCA lutetium (JSC, Russia) the radiopharmaceutical specific activity was 74 MBq/ug. For CA lutetium (IDB, Netherlands), the specific activity was 41 MBq/ug. The radiochemical purity was analyzed with HPLC. For all experiments, 6-well plates were used for adherence cells with 200,000 LNCaP per well. Molar concentration of saturation curves experiments were 0.01; 0.05; 0.6; 1.5; 3.0 and 3.5 for CA lutetium and 0.1; 0.6; 1.5; 2.0; 2.5 and 3.0 for NCA lutetium. After 1 hour of incubation at 8 ºC, supernatant was removed, then washed with PBS (phosphate buffer saline) and finally cells were burst with NaOH 1 M, and activity was measured in gama counter; the experiments were performed in octuplicate. Competition experiments were performed adding in all wells 5 nM of radiolabeled PSMA-617 and in the competition well (non-specific binding) were added an excess of 15 times (76 ug) of non radiolabeled PSMA-617. After 1 hour of incubation at 8 ºC, supernatant was removed, then washed with PBS and finally cells were burst with NaOH 1 M, and activity was measured in gama counter, these experiments were performed in triplicate. The specific binding was obtained by the difference between total binding and non-specific binding. Internalization experiments were performed at Kd of NCA and CA lutetium. After 1 hour of incubation at 37 ºC, supernatant was removed, washed with PBS, then washed again with 0.05 M glycine solution pH 2.8 and finally cells were burst with NaOH 1 M. Activity was measured in gama counter, these experiment were performed in sextuplicate. Results and discussion: The radiochemical purity were 98% and 99% for labeling with NCA and CA lutetium, respectively. Saturation curve assay with NCA lutetium shown a Kd of 0.7 ± 0.15 nM and a Bmax of 857 ± 55.79 pMol/ng, and with CA lutetium resulted in a Kd of 1.71 ± 0.45 nM and a Bmax of 1156 ± 113.8 pMol/ng. The variation between both Kd curves were statistically different (P value = 0.0058). Competition assay demonstrated an effective blocking for both types of lutetium, for NCA unpaired T test shown a P value of 0.0011. For CA lutetium, the unpaired test disclosed a P value of 0.0258. The comparison between both results revealed a P value of 0.01 at the specific binding. Internalization assay shown for both types of lutetium similar results, 27.1 ± 2.45% and 30.6 ± 4.97%, for CA and NCA lutetium, respectively, and was not statistically significant (P value = 0.17). Conclusions: These experiments demonstrated that the type of lutetium (CA or NCA) directly affects in vitro binding of 177Lu-PSMA-617 to receptors expressed in LNCaP cells. It was statistically demonstrated that the higher specific activity of 177Lu-PSMA-617, more radiolabeled peptide can bind to cells at saturation and competition assays.
Palavras-Chave:
radiopharmaceuticals;
lutetium 177;
therapy;
in vitro
VILLAS BOAS, CRISTIAN A.W.; MENGATTI, JAIR; PASSOS, PRISCILA; VIEIRA, DANIEL; ARAUJO, ELAINE B. de.
In vitro response of 177Lu-PSMA-617 with two different specific activities.
Journal of Nuclear Medicine,
v. 61,
2020.
supplement 1.
Disponível em: http://200.136.52.105/handle/123456789/31674. Acesso em: $DATA.
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COELHO, GUILHERME R.; PREZOTTO NETO, JOSE P.
; BARBOSA, FERNANDA C.; SANTOS, RAFAEL S.; BRIGATTE, PATRICIA; SPENCER, PATRICK J.
; SAMPAIO, SANDRA C.; D'AMELIO, FERNANDA; PIMENTA, DANIEL C.; SCIANI, JULIANA M..
Hypanus americanus mucus: a new point of view about stingray immunity and toxins.
Toxicon,
v. 177,
Supplement 1,
p. S34-S34,
2020.
DOI:
10.1016/j.toxicon.2019.12.047
Abstract:
Fish skin plays important biological roles, such as the control of the osmotic
pressure gradient, protection against mechanical forces and microorganism
infections. The mucus, on the other hand, is a rich and complex
fluid, important for the fish acting as innate immunity system, swimming
and nutrition. The elasmobranch epidermis is characterized mainly by
mucus secretory cells, and marine stingrays have already been described to
present secretory glands spread throughout the body. Little is known
about the biochemical composition of the stingray mucus, but recent
studies denoted the importance of mucus in the envenomation process.
Stingrays venom are largely studied due the human medical importance of
envenoming caused by sting puncture, that evolve with local inflammation
and necrosis, and these toxic events can be correlated to the chemical
composition of the sting skin, according to the literature. Aiming to analyse the mucus composition, a new non-invasive mucus collection method was
developed that focused on peptides and proteins, and biological assays
were performed to analyze preliminary toxic and immune activities of the
Hypanus americanus mucus. Pathophysiological characterization showed
the presence of peptidases on mucus, as well that the induction of edema
and leukocyte recruitment in mice. The fractionated mucus improved
phagocytosis on macrophages and showed antimicrobial activity against T.
rubrum, C. neoformans and C. albicans in vitro. The proteomic analyses
showed the presence of immune-related proteins like actin, histones,
hemoglobin, and ribosomal proteins. This protein pattern is similar to
those reported for other fish mucus and stingray venom. This is the first
report depicting the Hypanus stingray mucus composition, highlighting its
biochemical composition and importance for the stingray immune system
and the possible role on the envenomation process.
Palavras-Chave:
fishes;
skin;
venoms;
therapeutic uses
COELHO, GUILHERME R.; PREZOTTO NETO, JOSE P.; BARBOSA, FERNANDA C.; SANTOS, RAFAEL S.; BRIGATTE, PATRICIA; SPENCER, PATRICK J.; SAMPAIO, SANDRA C.; D'AMELIO, FERNANDA; PIMENTA, DANIEL C.; SCIANI, JULIANA M.
Hypanus americanus mucus: a new point of view about stingray immunity and toxins.
Toxicon,
v. 177,
p. S34-S34,
2020.
Supplement 1,
DOI:
10.1016/j.toxicon.2019.12.047.
Disponível em: http://200.136.52.105/handle/123456789/31673. Acesso em: $DATA.
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SABINO, CAETANO P.; SELLERA, FABIO P.; SALES-MEDINA, DOUGLAS F.; MACHADO, RAFAEL R.G.; DURIGON, EDISON L.; FREITAS-JUNIOR, LUCIO H.; RIBEIRO, MARTHA S.
.
UV-C (254 nm) lethal doses for SARS-CoV-2.
Photodiagnosis and Photodynamic Therapy,
v. 32,
p. 1-2,
2020.
DOI:
10.1016/j.pdpdt.2020.101995
Palavras-Chave:
sterilization;
germicides;
radiation doses;
ultraviolet radiation;
coronaviruses
SABINO, CAETANO P.; SELLERA, FABIO P.; SALES-MEDINA, DOUGLAS F.; MACHADO, RAFAEL R.G.; DURIGON, EDISON L.; FREITAS-JUNIOR, LUCIO H.; RIBEIRO, MARTHA S.
UV-C (254 nm) lethal doses for SARS-CoV-2.
Photodiagnosis and Photodynamic Therapy,
v. 32,
p. 1-2,
2020.
DOI:
10.1016/j.pdpdt.2020.101995.
Disponível em: http://200.136.52.105/handle/123456789/31672. Acesso em: $DATA.
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CASSOL, HENRIQUE L.G.; DOMINGUES, LUCAS G.
; BASSO, LUANA S.; GATTI, LUCIANA V.
; MARANI, LUCIANO; TEJADA, GRACIELA; ARAI, EGIDIO; MILLER, JOHN B.; ANDERSON, LIANA O.; ARAGAO, LUIZ E.O.C..
Understanding the temporal dynamics of carbon emission from fires in the Amazon-Cerrado transition zone.
Biodiversidade Brasileira,
v. 9,
n. 1,
p. 132-132,
2019.
DOI:
10.37002/biobrasil.v%25vi%25i.1273
Abstract:
Carbon emissions from fires (C fire) account for one-tenth of the global annual C
emissions. Fires are the main source of emissions from land-use change. Recently, Werf et al.
2017, showed an 11% increase in global fire emissions during the 1997-2016 period. Savannas
and Tropical Forests have shared almost the same fire-derived C emission values in the South
America (Werf et al. 2017). Therefore, for this study we focused our analysis on a site
representing the transition zone between Amazonia and Cerrado, located in Alta Floresta (ALF)
in Mato Grosso State, Brazil. To understand the temporal dynamics of fire-derived C emission,
we correlated fire counts (FC) within the influence area with fire-derived C emission directly
measured in the atmosphere as CO concentration. CO and CO2 were collected monthly for 7
years from 2010 onwards using an aircraft flying up to 4.5 km altitude, totaling 153 vertical profiles.
FC was extracted from influence areas weighted by the density of backward trajectories
calculated quarterly. Trajectories starting from the flight location within a cell of one degree
resolution were obtained using the Hysplit model at different heights. The average annual C
emission from fire was 0.10 ± 0.04 Pg.C.yr-1, which represents about 10% of the Amazon fire
emissions (Aragão et al. 2018). The highest daily value of fire emission was observed in 2010
(drought year) and 2017 (0.47-0.51 gC.m².day-1), although the years of 2011, 2016, and 2017
were the largest contributor to the total emission flux. This occurred because the influence area
that belongs to the Amazon was lower in those years. Inter annually there is a typical behavior of
the backward trajectories in ALF site whose 1st and 4th quarters have 80% of the air-streams
coming from the Amazon, while during the 2nd and 3rd quarters this contribution is about 45%.
However, emissions from fire and FC are higher in the 3rd quarter, outside the Amazon. The
correlation between FC and fire emission was positively significant (ρ = 0.88, α = 0.05, p<0.001),
meaning that an increase of 1,000 FC per quarter causes an increase of 0.074 gC.m².day-1 or, on
average, an emission of 0.16 Pg.C.yr-1.
Palavras-Chave:
fires;
carbon dioxide;
emission;
forests;
greenhouse gases;
environment
CASSOL, HENRIQUE L.G.; DOMINGUES, LUCAS G.; BASSO, LUANA S.; GATTI, LUCIANA V.; MARANI, LUCIANO; TEJADA, GRACIELA; ARAI, EGIDIO; MILLER, JOHN B.; ANDERSON, LIANA O.; ARAGAO, LUIZ E.O.C.
Understanding the temporal dynamics of carbon emission from fires in the Amazon-Cerrado transition zone.
Biodiversidade Brasileira,
v. 9,
n. 1,
p. 132-132,
2019.
DOI:
10.37002/biobrasil.v%25vi%25i.1273.
Disponível em: http://200.136.52.105/handle/123456789/31671. Acesso em: $DATA.
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-
TEJADA, GRACIELA; GATTI, LUCIANA
; BASSO, LUANA; CASSOL, HENRIQUE L.G.; MARANI, LUCIANO; CORREIA, CAIO
; DOMINGUES, LUCAS
; CRISPIM, STEPHANE; NEVES, RAIANE; ANDERSON, LIANA O.; ARAGAO, LUIZ E.O.C.; ARAI, EGIDIO; GLOOR, MANUEL; MILLER, JOHN B.; VON RANDOW, CELSO.
Understanding the relationships between local deforestation and CO2 atmospheric measurements in the Brazilian Amazon.
Pesquisa Florestal Brasileira - Brazilian Journal of Forestry Research,
v. 39,
Special issue,
p. 528-528,
2019.
DOI:
10.4336/2019.pfb.39e201902043
Abstract:
Amazon forests play a fundamental role in the global carbon balance as a carbon sink, but temperature elevations and frequents extreme events as droughts and
floods could make the forests a source of CO2. Local atmospheric measurements of greenhouse gases are needed to better understand how forest will respond to
climate change. The lower-troposphere greenhouse gas (GHG) monitoring program over Brazilian Amazon Basin, has been collecting biweekly GHGs vertical
profiles in four sites since 2010. We aim to understand the relationships between local deforestation and CO2 aircraft measurements in the Brazilian Amazon. We
calculated annual deforestation (using the Amazon Deforestation Calculation Program - PRODES), land use and cover change data (using the Brazilian Institute
of Geography and Statistics - IBGE) and fire data (using the Fire Monitoring System) in each annual influence area at the four flight measurement sites of the
Brazilian Amazon from 2010-2017 (and also in the mean influence area of all years by sites). We found that when we see total deforestation, it has a relationship
with global CO2 emissions in the Brazilian Amazon biome. Fire has a strong relationship in the drought years, mostly in 2012. Looking at each site, we found
specific correlations with deforestation, fire and land use. The biggest challenge was to compare spatial analyzes of land use change and fire with punctual data
of airplane GHGs measurements. This study will contribute in our understanding of anthropogenic activities over the Amazon forest in a changing climate.
Palavras-Chave:
deforestation;
greenhouse gases;
monitoring;
environment;
atmospheres;
carbon dioxide;
emission
TEJADA, GRACIELA; GATTI, LUCIANA; BASSO, LUANA; CASSOL, HENRIQUE L.G.; MARANI, LUCIANO; CORREIA, CAIO; DOMINGUES, LUCAS; CRISPIM, STEPHANE; NEVES, RAIANE; ANDERSON, LIANA O.; ARAGAO, LUIZ E.O.C.; ARAI, EGIDIO; GLOOR, MANUEL; MILLER, JOHN B.; VON RANDOW, CELSO.
Understanding the relationships between local deforestation and CO2 atmospheric measurements in the Brazilian Amazon.
Pesquisa Florestal Brasileira - Brazilian Journal of Forestry Research,
v. 39,
p. 528-528,
2019.
Special issue,
DOI:
10.4336/2019.pfb.39e201902043.
Disponível em: http://200.136.52.105/handle/123456789/31670. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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BASSO, LUANA S.; GATTI, LUCIANA V.
; MARANI, LUCIANO; CASSOL, HENRIQUE L.G.; TEJADA, GRACIELA; CORREIA, CAIO S.C.
; DOMINGUES, LUCAS G.
; CRISPIM, STEPHANE P.; NEVES, RAIANE A.L.; ARAI, EGIDIO; MILLER, JOHN B.; GLOOR, MANUEL; ANDERSON, LIANA O.; ARAGAO, LUIZ E.O.C..
A first Amazon CH4 budget and its controls based on atmospheric data from vertical profiles measurements.
Pesquisa Florestal Brasileira - Brazilian Journal of Forestry Research,
v. 39,
Special issue,
p. 528-528,
2019.
DOI:
10.4336/2019.pfb.39e201902043
Abstract:
The role of tropical regions in the global CH4 balance remained uncertain, due these regions have until recently been poorly observed with large-scale integrating
in-situ observations. To contribute in understanding the CH4 balance in tropical regions, we have started a lower-troposphere greenhouse gas-monitoring program
over Brazilian Amazon Basin consisting of regular vertical profile greenhouse gas observations at four sites. Samples are collected regularly each 2 weeks, using
light aircraft. We will present an analysis of these data and what they tell us about the Amazon CH4 cycle and its contribution to global CH4 concentration. We
estimate fluxes upwind of the sites from the profile data using a column budgeting approach (Miller et al., 2007). Over the full period (2010-2017) the Amazon
Basin was a source of CH4, but with regional variations. There are comparably high and highly variable emissions from the eastern part of the basin exhibiting
strong variability, with particularly high CH4 fluxes in two different periods of the year (beginning of the wet season and in the dry season). In contrast to this,
a clear seasonality was observed at the other three sites, with the largest emissions occurring at the beginning of the wet season (January to March). Emissions
from biomass burning contribute with a small part of the total flux at each site. We will finally discuss what is the influence from precipitation and temperature
in the Amazon CH4 emissions.
Palavras-Chave:
methane;
atmospheres;
carbon monoxide;
greenhouse gases;
wetlands
BASSO, LUANA S.; GATTI, LUCIANA V.; MARANI, LUCIANO; CASSOL, HENRIQUE L.G.; TEJADA, GRACIELA; CORREIA, CAIO S.C.; DOMINGUES, LUCAS G.; CRISPIM, STEPHANE P.; NEVES, RAIANE A.L.; ARAI, EGIDIO; MILLER, JOHN B.; GLOOR, MANUEL; ANDERSON, LIANA O.; ARAGAO, LUIZ E.O.C.
A first Amazon CH4 budget and its controls based on atmospheric data from vertical profiles measurements.
Pesquisa Florestal Brasileira - Brazilian Journal of Forestry Research,
v. 39,
p. 528-528,
2019.
Special issue,
DOI:
10.4336/2019.pfb.39e201902043.
Disponível em: http://200.136.52.105/handle/123456789/31669. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
Como referenciar este item
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A elaboração do projeto do RI do IPEN foi iniciado em novembro de 2013, colocado em operação interna em julho de 2014 e disponibilizado na Internet em junho de 2015. Utiliza o software livre Dspace, desenvolvido pelo Massachusetts Institute of Technology (MIT). Para descrição dos metadados adota o padrão Dublin Core. É compatível com o Protocolo de Arquivos Abertos (OAI) permitindo interoperabilidade com repositórios de âmbito nacional e internacional.
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